Max Rudolph, Christian Schmeer, Madlen Günther, Florus Woitke, Carolin Kathner-Schaffert, Lina Karapetow, Julia Lindner, Thomas Lehmann, Gustav Jirikowski, Otto W. Witte, Christoph Redecker, Silke Keiner
- Following stroke, neuronal death takes place both in the infarct region and in brain areas distal to the lesion site including the hippocampus. The hippocampus is critically involved in learning and memory processes and continuously generates new neurons. Dysregulation of adult neurogenesis may be associated with cognitive decline after a stroke lesion. In particular, proliferation of precursor cells and the formation of new neurons are increased after lesion. Within the first week, many new precursor cells die during development. How dying precursors are removed from the hippocampus and to what extent phagocytosis takes place after stroke is still not clear. Here, we evaluated the effect of a prefrontal stroke lesion on the phagocytic activity of microglia in the dentate gyrus (DG) of the hippocampus. Three-months-old C57BL/6J mice were injected once with the proliferation marker BrdU (250 mg/kg) 6 hr after a middle cerebral artery occlusion or sham surgery. The number of apoptotic cells and the phagocytic capacity of the microgliaFollowing stroke, neuronal death takes place both in the infarct region and in brain areas distal to the lesion site including the hippocampus. The hippocampus is critically involved in learning and memory processes and continuously generates new neurons. Dysregulation of adult neurogenesis may be associated with cognitive decline after a stroke lesion. In particular, proliferation of precursor cells and the formation of new neurons are increased after lesion. Within the first week, many new precursor cells die during development. How dying precursors are removed from the hippocampus and to what extent phagocytosis takes place after stroke is still not clear. Here, we evaluated the effect of a prefrontal stroke lesion on the phagocytic activity of microglia in the dentate gyrus (DG) of the hippocampus. Three-months-old C57BL/6J mice were injected once with the proliferation marker BrdU (250 mg/kg) 6 hr after a middle cerebral artery occlusion or sham surgery. The number of apoptotic cells and the phagocytic capacity of the microglia were evaluated by means of immunohistochemistry, confocal microscopy, and 3D-reconstructions. We found a transient but significant increase in the number of apoptotic cells in the DG early after stroke, associated with impaired removal by microglia. Interestingly, phagocytosis of newly generated precursor cells was not affected. Our study shows that a prefrontal stroke lesion affects phagocytosis of apoptotic cells in the DG, a region distal to the lesion core. Whether disturbed phagocytosis might contribute to inflammatory- and maladaptive processes including cognitive impairment following stroke needs to be further investigated.…
MetadatenAuthor details: | Max RudolphGND, Christian SchmeerORCiDGND, Madlen Günther, Florus Woitke, Carolin Kathner-Schaffert, Lina Karapetow, Julia Lindner, Thomas Lehmann, Gustav JirikowskiGND, Otto W. Witte, Christoph Redecker, Silke KeinerORCiDGND |
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DOI: | https://doi.org/10.1002/glia.24009 |
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ISSN: | 0894-1491 |
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ISSN: | 1098-1136 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/33942391 |
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Title of parent work (English): | Glia |
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Publisher: | Wiley-Blackwell |
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Place of publishing: | Hoboken |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2021/08/05 |
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Publication year: | 2021 |
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Release date: | 2024/02/29 |
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Tag: | MCAO; activated caspase 3; dentate gyrus; neurogenesis; pyknotic cells |
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Volume: | 69 |
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Issue: | 8 |
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Number of pages: | 17 |
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First page: | 2006 |
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Last Page: | 2022 |
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Funding institution: | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [Ke1914/2-1]; Else Kroner-Fresenius-Stiftung |
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Organizational units: | Fakultät für Gesundheitswissenschaften |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 590 Tiere (Zoologie) |
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| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Peer review: | Referiert |
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License (German): | CC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International |
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