Anne Margarete Merks, Marie Swinarski, Alexander Matthias Meyer, Nicola Victoria Müller, Ismail Özcan, Stefan Donat, Alexa Burger, Stephen Gilbert, Christian Mosimann, Salim Abdelilah-Seyfried, Daniela Panakova
- Development of a multiple-chambered heart from the linear heart tube is inherently linked to cardiac looping. Although many molecular factors regulating the process of cardiac chamber ballooning have been identified, the cellular mechanisms underlying the chamber formation remain unclear. Here, we demonstrate that cardiac chambers remodel by cell neighbour exchange of cardiomyocytes guided by the planar cell polarity (PCP) pathway triggered by two non-canonical Wnt ligands, Wnt5b and Wnt11. We find that PCP signalling coordinates the localisation of actomyosin activity, and thus the efficiency of cell neighbour exchange. On a tissue-scale, PCP signalling planar-polarises tissue tension by restricting the actomyosin contractility to the apical membranes of outflow tract cells. The tissue-scale polarisation of actomyosin contractility is required for cardiac looping that occurs concurrently with chamber ballooning. Taken together, our data reveal that instructive PCP signals couple cardiac chamber expansion with cardiac looping throughDevelopment of a multiple-chambered heart from the linear heart tube is inherently linked to cardiac looping. Although many molecular factors regulating the process of cardiac chamber ballooning have been identified, the cellular mechanisms underlying the chamber formation remain unclear. Here, we demonstrate that cardiac chambers remodel by cell neighbour exchange of cardiomyocytes guided by the planar cell polarity (PCP) pathway triggered by two non-canonical Wnt ligands, Wnt5b and Wnt11. We find that PCP signalling coordinates the localisation of actomyosin activity, and thus the efficiency of cell neighbour exchange. On a tissue-scale, PCP signalling planar-polarises tissue tension by restricting the actomyosin contractility to the apical membranes of outflow tract cells. The tissue-scale polarisation of actomyosin contractility is required for cardiac looping that occurs concurrently with chamber ballooning. Taken together, our data reveal that instructive PCP signals couple cardiac chamber expansion with cardiac looping through the organ-scale polarisation of actomyosin-based tissue tension.…
MetadatenAuthor details: | Anne Margarete MerksORCiD, Marie SwinarskiORCiD, Alexander Matthias MeyerORCiD, Nicola Victoria MüllerORCiD, Ismail ÖzcanORCiD, Stefan DonatORCiD, Alexa Burger, Stephen Gilbert, Christian MosimannORCiD, Salim Abdelilah-SeyfriedORCiDGND, Daniela PanakovaORCiD |
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DOI: | https://doi.org/10.1038/s41467-018-04566-1 |
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ISSN: | 2041-1723 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29867082 |
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Title of parent work (English): | Nature Communications |
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Publisher: | Nature Publ. Group |
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Place of publishing: | London |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2018/06/04 |
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Publication year: | 2018 |
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Release date: | 2021/11/22 |
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Volume: | 9 |
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Number of pages: | 15 |
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Funding institution: | Helmholtz Young Investigator Program [VH-NG-736]; Marie Curie Career Integration Grant from the European Commission (MC CIG) [WNT/CALCIUM IN HEART-322189]; excellence cluster REBIRTH [SFB958]; DFGGerman Research Foundation (DFG) [SE2016/10-1, PA2619/1-1]; Canton of Zurich; MC CIG [PCIG14-GA-2013-631984]; Swiss Heart Foundation; Swiss National Science Foundation (SNSF) professorshipSwiss National Science Foundation (SNSF) [PP00P3_139093] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik |
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License (German): | CC-BY - Namensnennung 4.0 International |
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External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 849 |
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