Ion-Selective formation of a guanine quadruplex on DNA origami structures
- DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na+ and K+) is currently used for the detection of K+ ions, however, with insufficient selectivity towards Na+. By means of FRET between two suitable dyes attached to the 3- and 5-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.
Verfasserangaben: | Lydia OlejkoORCiDGND, Piotr J. Cywinski, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1002/anie.201409278 |
ISSN: | 1433-7851 |
ISSN: | 1521-3773 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/25413669 |
Titel des übergeordneten Werks (Englisch): | Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2015 |
Erscheinungsjahr: | 2015 |
Datum der Freischaltung: | 27.03.2017 |
Freies Schlagwort / Tag: | DNA nanotechnology; FRET; G-quadruplexes; nanostructures; self-assembly |
Band: | 54 |
Ausgabe: | 2 |
Seitenanzahl: | 5 |
Erste Seite: | 673 |
Letzte Seite: | 677 |
Fördernde Institution: | Marie Curie FP7 Integration Grant within the 7th European Union Framework Programme; Deutsche Forschungsgemeinschaft (DFG); University of Potsdam; DFG [GSC 1013] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer Review: | Referiert |