Placement of Single Proteins within the SERS Hot Spots of Self-Assembled Silver Nanolenses
- This study demonstrates the bottom-up synthesis of silver nanolenses. A robust coating protocol enabled the functionalization of differently sized silver nanoparticles with DNA single strands of orthogonal sequence. Coated particles 10nm, 20nm, and 60nm in diameter were self-assembled by DNA origami scaffolds to form silver nanolenses. Single molecules of the protein streptavidin were selectively placed in the gap of highest electric field enhancement. Streptavidin labelled with alkyne groups served as model analyte in surface-enhanced Raman scattering (SERS) experiments. By correlated Raman mapping and atomic force microscopy, SERS signals of the alkyne labels of a single streptavidin molecule, from a single silver nanolens, were detected. The discrete, self-similar aggregates of solid silver nanoparticles are promising for plasmonic applications.
Author details: | Christian HeckORCiDGND, Yuya KanehiraGND, Janina KneippORCiD, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1002/anie.201801748 |
ISSN: | 1433-7851 |
ISSN: | 1521-3773 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29575472 |
Title of parent work (English): | Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition |
Publisher: | Wiley-VCH |
Place of publishing: | Weinheim |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/03/25 |
Publication year: | 2018 |
Release date: | 2021/11/19 |
Tag: | DNA origami; SERS; protein analysis; silver nanoparticles; streptavidin |
Volume: | 57 |
Issue: | 25 |
Number of pages: | 4 |
First page: | 7444 |
Last Page: | 7447 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG); European Regional Development Fund (EFRE)European Union (EU); University of Potsdam; Federal Institute of Materials Research (BAM); ERCEuropean Research Council (ERC) [259432]; DFGGerman Research Foundation (DFG) [GSC 1013] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |