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Analytical epigenetics
- The field of epigenetics describes the relationship between genotype and phenotype, by regulating gene expression without changing the canonical base sequence of DNA. It deals with molecular genomic information that is encoded by a rich repertoire of chemical modifications and molecular interactions. This regulation involves DNA, RNA and proteins that are enzymatically tagged with small molecular groups that alter their physical and chemical properties. It is now clear that epigenetic alterations are involved in development and disease, and thus, are the focus of intensive research. The ability to record epigenetic changes and quantify them in rare medical samples is critical for next generation diagnostics. Optical detection offers the ultimate single-molecule sensitivity and the potential for spectral multiplexing. Here we review recent progress in ultrasensitive optical detection of DNA and histone modifications.
Author details: | Christian HeckORCiDGND, Yael Michaeli, Ilko BaldORCiDGND, Yuval EbensteinORCiD |
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DOI: | https://doi.org/10.1016/j.copbio.2018.09.006 |
ISSN: | 0958-1669 |
ISSN: | 1879-0429 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30326408 |
Title of parent work (English): | Current Opinion in Biotechnology |
Subtitle (English): | single-molecule optical detection of DNA and histone modifications |
Publisher: | Elsevier |
Place of publishing: | London |
Publication type: | Review |
Language: | English |
Year of first publication: | 2018 |
Publication year: | 2018 |
Release date: | 2021/04/14 |
Volume: | 55 |
Number of pages: | 8 |
First page: | 151 |
Last Page: | 158 |
Funding institution: | BeyondSey consortium (EC program) [634890]; European Research Council starter grant [337830]; European Research Council Proof of Concept grant by the EC-Horizon2020 program [767931]; NIH-R21 grant [1R21ES028015-01]; ERC Consolidator Grant [772752]; German Research Foundation (DEG)German Research Foundation (DFG) [BA4026/5-2] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access / Green Open-Access |