Dual-Stimuli Sensitive Hybrid Materials: Ferritin-PDMAEMA by Grafting-From Polymerization
- The combination of stimuli-responsive polymers and proteins that can transport drugs is a promising approach for drug delivery. The formation of ferritin-poly(2-dimethylaminoethyl methacrylate) (PDMAEMA) conjugates by atom-transfer radical polymerization from the protein macroinitiator is described. PDMAEMA is a dual-stimuli-responsive polymer and the thermo- and pH-responsive properties of the resulting conjugates are studied in detail with dynamic light scattering (DLS). Additionally, it is demonstrated that the lower critical solution temperature (LCST) of the protein-polymer conjugates can be further adjusted by the ionic strength of the solution. The conjugates are also characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) mass spectrometry, and NMR spectroscopy. The obtained MALDI-ToF mass spectra are exceptional for protein-polymer conjugates and have not been so often reported.
Author details: | Marli Luiza Tebaldi, Himanshu CharanORCiDGND, Liliia Mavliutova, Alexander BökerORCiDGND, Ulrich GlebeORCiDGND |
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DOI: | https://doi.org/10.1002/macp.201600529 |
ISSN: | 1022-1352 |
ISSN: | 1521-3935 |
Title of parent work (English): | Macromolecular chemistry and physics |
Publisher: | Wiley-VCH |
Place of publishing: | Weinheim |
Publication type: | Article |
Language: | English |
Year of first publication: | 2017 |
Publication year: | 2017 |
Release date: | 2020/04/20 |
Tag: | MALDI-ToF MS; grafting-from; polymerization; proteins; responsivity |
Volume: | 218 |
Number of pages: | 6 |
Funding institution: | CNPq; Bundesministerium fur Bildung und Forschung (BMBF) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |