Utility of an appropriate reporter assay: Heliotrine interferes with GAL4/upstream activation sequence-driven reporter gene systems
- Reporter gene assays are widely used for the assessment of transcription factor activation following xenobiotic exposure of cells. A critical issue with such assays is the possibility of interference of test compounds with the test system, for example, by direct inhibition of the reporter enzyme. Here we show that the pyrrolizidine alkaloid heliotrine interferes with reporter signals derived from GAL4-based nuclear receptor transactivation assays by a mechanism independent of luciferase enzyme inhibition. These data highlight the necessity to conduct proper control experiments in order to avoid perturbation of reporter assays by test chemicals. (C) 2015 Elsevier Inc. All rights reserved.
| Verfasserangaben: | Claudia Luckert, Stefanie Hessel, Alfonso Lampen, Albert Braeuning |
|---|---|
| DOI: | https://doi.org/10.1016/j.ab.2015.07.009 |
| ISSN: | 0003-2697 |
| ISSN: | 1096-0309 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26212314 |
| Titel des übergeordneten Werks (Englisch): | Analytical biochemistry : methods in the biological sciences |
| Verlag: | Elsevier |
| Verlagsort: | San Diego |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2015 |
| Erscheinungsjahr: | 2015 |
| Datum der Freischaltung: | 27.03.2017 |
| Freies Schlagwort / Tag: | Firefly luciferase inhibition; Nuclear receptor; Transactivation assay |
| Band: | 487 |
| Seitenanzahl: | 4 |
| Erste Seite: | 45 |
| Letzte Seite: | 48 |
| Fördernde Institution: | Federal Institute for Risk Assessment [51-006, 1322-454, 1322-591] |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
| Peer Review: | Referiert |
