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Products of in vitro oxidation of N-acetyldopamine as possible components in the sclerotization of insect cuticle
- [1-14C]-N-Acetyldopamine (NADA) was oxidized in the presence of methyl [3-3H]-β-alanate with mushroom tyrosinase. The complex mixture of reaction products was partly resolved by chromatographic procedures and analyzed by spectroscopic methods. Methyl-β-alanate is incorporated to only a small extent into oxidation products of NADA which inter alia are presumed to be oligomeric hydroxyquinones. After oxidation of [1-14C, 2-3H]-NADA with preparations from tanning Manduca sexta pupal cuticle, N-acetylnoradrenalin was identified as one of the products. Binding of radioactivity to melanin-like material was also observed. These results suggest that oxidation products different from those formulated usually for the crosslinkages between protein amino groups and N-acetyldopaquinone are deposited in darkly brown coloured insect cuticles during sclerotization.
Author details: | Martin G. Peter |
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URN: | urn:nbn:de:kobv:517-opus-16759 |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 041) |
Publication type: | Postprint |
Language: | English |
Publication year: | 1980 |
Publishing institution: | Universität Potsdam |
Release date: | 2008/03/14 |
Tag: | N-acetyldopamine; sclerotization; tanning agents; tyrosinase; o-quinones |
Source: | Insect Biochemistry. - ISSN 0965-1748. - 10 (1980), 2, p. 221 - 227 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
License (German): | Keine öffentliche Lizenz: Unter Urheberrechtsschutz |
External remark: | first published in: Insect Biochemistry - 10 (1980), 2 p. 221 - 227 ISSN: 0965-1748 doi: 10.1016/0020-1790(80)90077-3 |