- Owing their unique chemical and physical properties core-multishell (CMS) nanocarriers are thought to underlie their exploitable biomedical use for a topical treatment of skin diseases. This highlights the need to consider not only the efficacy of CMS nanocarriers but also the potentially unpredictable and adverse consequences of their exposure thereto. As CMS nanocarriers are able to penetrate into viable layers of normal and stripped human skin ex vivo as well as in in vitro skin disease models the understanding of nanoparticle crosstalk with components of the immune system requires thorough investigation. Our studies highlight the biocompatible properties of CMS nanocarriers on Langerhans cells of the skin as they did neither induce cytotoxicity and genotoxicity nor cause reactive oxygen species (ROS) or an immunological response. Nevertheless, CMS nanocarriers were efficiently taken up by Langerhans cells via divergent endocytic pathways. Bioimaging of CMS nanocarriers by fluorescence lifetime imaging microscopy (FLIM) and flowOwing their unique chemical and physical properties core-multishell (CMS) nanocarriers are thought to underlie their exploitable biomedical use for a topical treatment of skin diseases. This highlights the need to consider not only the efficacy of CMS nanocarriers but also the potentially unpredictable and adverse consequences of their exposure thereto. As CMS nanocarriers are able to penetrate into viable layers of normal and stripped human skin ex vivo as well as in in vitro skin disease models the understanding of nanoparticle crosstalk with components of the immune system requires thorough investigation. Our studies highlight the biocompatible properties of CMS nanocarriers on Langerhans cells of the skin as they did neither induce cytotoxicity and genotoxicity nor cause reactive oxygen species (ROS) or an immunological response. Nevertheless, CMS nanocarriers were efficiently taken up by Langerhans cells via divergent endocytic pathways. Bioimaging of CMS nanocarriers by fluorescence lifetime imaging microscopy (FLIM) and flow cytometry indicated not only a localization within the lysosomes but also an energy-dependent exocytosis of unmodified CMS nanocarriers into the extracellular environment. (C) 2018 Elsevier Ltd. All rights reserved.…
MetadatenAuthor details: | Alexander EdlichGND, Pierre Volz, Robert Brodwolf, Michael Unbehauen, Lars Mundhenk, Achim D. Gruber, Sarah HedtrichORCiD, Rainer Haag, Ulrike Alexiev, Burkhard KleuserORCiDGND |
---|
DOI: | https://doi.org/10.1016/j.biomaterials.2018.01.058 |
---|
ISSN: | 0142-9612 |
---|
ISSN: | 1878-5905 |
---|
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29438881 |
---|
Title of parent work (English): | Biomaterials : biomaterials reviews online |
---|
Publisher: | Elsevier |
---|
Place of publishing: | Oxford |
---|
Publication type: | Article |
---|
Language: | English |
---|
Date of first publication: | 2018/02/03 |
---|
Publication year: | 2018 |
---|
Release date: | 2021/12/14 |
---|
Tag: | Core-multishell nanocarriers; Fluorescence lifetime imaging microscopy; Langerhans cells; Nanoparticle uptake; Nanotoxicology |
---|
Volume: | 162 |
---|
Number of pages: | 11 |
---|
First page: | 60 |
---|
Last Page: | 70 |
---|
Funding institution: | German Research Foundation (DFG) Collaborative Research CenterGerman Research Foundation (DFG) [(SFB) 1112] |
---|
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
---|
DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
---|
Peer review: | Referiert |
---|