- search hit 1 of 1
Development, validation and application of an ICP-MS/MS method to quantify minerals and (ultra-)trace elements in human serum
- Multi-element determination in human samples is very challenging. Especially in human intervention studies sample volumes are often limited to a few microliters and due to the high number of samples a high-throughput is indispensable. Here, we present a state-of-the-art ICP-MS/MS-based method for the analysis of essential (trace) elements, namely Mg, Ca, Fe, Cu, Zn, Mo, Se and I, as well as food-relevant toxic elements such as As and Cd. The developed method was validated regarding linearity of the calibration curves, method LODs and LOQs, selectivity and trueness as well as precision. The established reliable method was applied to quantify the element serum concentrations of participants of a human intervention study (LeguAN). The participants received isocaloric diets, either rich in plant protein or in animal protein. While the serum concentrations of Mg and Mo increased in participants receiving the plant protein-based diet (above all legumes), the Se concentration in serum decreased. In contrast, the animal protein-based diet,Multi-element determination in human samples is very challenging. Especially in human intervention studies sample volumes are often limited to a few microliters and due to the high number of samples a high-throughput is indispensable. Here, we present a state-of-the-art ICP-MS/MS-based method for the analysis of essential (trace) elements, namely Mg, Ca, Fe, Cu, Zn, Mo, Se and I, as well as food-relevant toxic elements such as As and Cd. The developed method was validated regarding linearity of the calibration curves, method LODs and LOQs, selectivity and trueness as well as precision. The established reliable method was applied to quantify the element serum concentrations of participants of a human intervention study (LeguAN). The participants received isocaloric diets, either rich in plant protein or in animal protein. While the serum concentrations of Mg and Mo increased in participants receiving the plant protein-based diet (above all legumes), the Se concentration in serum decreased. In contrast, the animal protein-based diet, rich in meat and dairy products, resulted in an increased Se concentration in serum.…
Author details: | Sören MeyerORCiDGND, Mariya MarkovaORCiDGND, Gabriele Pohl, Talke Anu Marschall, Olga PivovarovaORCiD, Andreas F. H. PfeifferORCiDGND, Tanja SchwerdtleORCiDGND |
---|---|
DOI: | https://doi.org/10.1016/j.jtemb.2018.05.012 |
ISSN: | 0946-672X |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29895367 |
Title of parent work (English): | Journal of trace elements in medicine and biology |
Publisher: | Elsevier GMBH |
Place of publishing: | München |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/05/30 |
Publication year: | 2018 |
Release date: | 2022/03/21 |
Tag: | Elemental blood serum concentration; Human nutritional intervention; ICP-MS |
Volume: | 49 |
Number of pages: | 7 |
First page: | 157 |
Last Page: | 163 |
Funding institution: | NutriAct - Competence Cluster Nutrition Research Berlin-Potsdam - Federal Ministry of Education and Research (BMBF) [FKZ: 01EA1408B]; German Research Foundation (DFG)German Research Foundation (DFG) [SCHW903/10-1]; TraceAge - DFG Research Unit on Interactions of essential trace elements in healthy and diseased elderly, Potsdam-Berlin-Jena [FOR 2558/1]; Federal Ministry of Food and Agriculture (BMEL) based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food (BLE) under the innovation support program (BLE) [2815407110]; German Center for Diabetes Research |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit |
Peer review: | Referiert |