Low proteasomal activity in fast skeletal muscle fibers is not associated with increased age-related oxidative damage
- The skeletal muscle is a crucial tissue for maintaining whole body homeostasis. Aging seems to have a disruptive effect on skeletal muscle homeostasis including proteostasis. However, how aging specifically impacts slow and fast twitch fiber types remains elusive. Muscle proteostasis is largely maintained by the proteasomal system. Here we characterized the proteasomal system in two different fiber types, using a non-sarcopenic aging model. By analyzing the proteasomal activity and amount, as well as the polyubiquitinated proteins and the level of protein oxidation in Musculus soleus (Sol) and Musculus extensor digitorum longus (EDL), we found that the slow twitch Sol muscle shows an overall higher respiratory and proteasomal activity in young and old animals. However, especially during aging the fast twitch EDL muscle reduces protein oxidation by an increase of antioxidant capacity. Thus, under adaptive non-sarcopenic conditions, the two fibers types seem to have different strategies to avoid age-related changes.
Author details: | Raquel Fernando, Cathleen Drescher, Stefanie Deubel, Tobias JungORCiDGND, Mario Ost, Susanne KlausORCiDGND, Tilman GruneORCiDGND, Jose Pedro CastroORCiD |
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DOI: | https://doi.org/10.1016/j.exger.2018.10.018 |
ISSN: | 0531-5565 |
ISSN: | 1873-6815 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30367978 |
Title of parent work (English): | Experimental gerontology |
Publisher: | Elsevier |
Place of publishing: | Oxford |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/10/24 |
Publication year: | 2018 |
Release date: | 2021/03/31 |
Tag: | Aging; Antioxidants; Fast and slow fibers; Mitochondrial respiration; Oxidized proteins; Polyubiquitination; Proteasomal system; Skeletal muscle |
Volume: | 117 |
Number of pages: | 8 |
First page: | 45 |
Last Page: | 52 |
Funding institution: | German Research Foundation (DFG)German Research Foundation (DFG) [Gr1240/18-1]; Ministerium fur Wissenschaft, Forschung und Kultur (Gesundheitscampus Brandenburg) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Peer review: | Referiert |