Two-Photon excitation fluorescence spectroscopy of quantum dots
- The applications of quantum dots (QDs) in two-photon (2P) excitation applications demand reliable data about their 2P absorption (2PA) cross sections (sigma(2PA)). In the present study, sigma(2PA) values have been determined for a series of commercial colloidal CdSe/ZnS QDs and CdSeTe/ZnS QDs in aqueous media. For the first time for these QDs, the sigma(2PA) values have been determined over a wide spectral range, that is, between 720 and 900 nm, and are compared to the extinction coefficient (epsilon) values obtained under one-photon (1P) excitation. Furthermore, we present a QD in combination with an organic dye in a biotin-streptavidin Forster resonance energy transfer bioassay under 1P and 2P excitation. The results for the bioassay under 2P excitation are compared to those obtained under 1P excitation. The results demonstrate that in the case of the 2P excitation, higher sensitivity can be achieved because of an improved signal-to-noise ratio.
Author details: | Till Thomas MeilingORCiD, Piotr J. Cywinski, Hans-Gerd LöhmannsröbenORCiDGND |
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DOI: | https://doi.org/10.1021/acs.jpcc.7b12345 |
ISSN: | 1932-7447 |
ISSN: | 1932-7455 |
Title of parent work (English): | The journal of physical chemistry : C, Nanomaterials and interfaces |
Subtitle (English): | photophysical properties and application in bioassays |
Publisher: | American Chemical Society |
Place of publishing: | Washington |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/05/03 |
Publication year: | 2018 |
Release date: | 2021/12/01 |
Volume: | 122 |
Issue: | 17 |
Number of pages: | 7 |
First page: | 9641 |
Last Page: | 9647 |
Funding institution: | FP7 Collaborative Project NANOGNOSTICS [HEALTH-F5-2009-242264] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |