Sensitizing DNA Towards Low-Energy Electrons with 2-Fluoroadenine
- 2-Fluoroadenine ((2F)A) is a therapeutic agent, which is suggested for application in cancer radiotherapy. The molecular mechanism of DNA radiation damage can be ascribed to a significant extent to the action of low-energy (<20 eV) electrons (LEEs), which damage DNA by dissociative electron attachment. LEE induced reactions in (2F)A are characterized both isolated in the gas phase and in the condensed phase when it is incorporated into DNA. Information about negative ion resonances and anion-mediated fragmentation reactions is combined with an absolute quantification of DNA strand breaks in (2F)A-containing oligonucleotides upon irradiation with LEEs. The incorporation of (2F)A into DNA results in an enhanced strand breakage. The strand-break cross sections are clearly energy dependent, whereas the strand-break enhancements by (2F)A at 5.5, 10, and 15 eV are very similar. Thus, (2F)A can be considered an effective radiosensitizer operative at a wide range of electron energies.
| Author details: | Jenny RackwitzGND, Janina KopyraORCiD, Iwona Dabkowska, Kenny EbelORCiD, MiloS Lj. Rankovic, Aleksandar R. Milosavljevic, Ilko BaldORCiDGND |
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| DOI: | https://doi.org/10.1002/anie.201603464 |
| ISSN: | 1433-7851 |
| ISSN: | 1521-3773 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/27481662 |
| Title of parent work (English): | Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2016 |
| Publication year: | 2016 |
| Release date: | 2020/03/22 |
| Tag: | DNA origami; DNA radiation damage; ab initio calculations; dissociative electron attachment; fludarabine |
| Volume: | 55 |
| Number of pages: | 5 |
| First page: | 10248 |
| Last Page: | 10252 |
| Funding institution: | Federal Institute for Materials Research and Testing (BAM); Polish Ministry of Science and Higher Education; Marie Curie FP7 Integration Grant within the 7th European Union Framework Programme; Deutsche Forschungsgemeinschaft (DFG); University of Potsdam; European Union via the COST Action [MP1002]; Foundation for Polish Science - POIG [POMOST/2012-6/3]; MESTD of the Republic of Serbia [171020] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Peer review: | Referiert |
