An ion-controlled four-color fluorescent telomeric switch on DNA origami structures
- The folding of single-stranded telomeric DNA into guanine (G) quadruplexes is a conformational change that plays a major role in sensing and drug targeting. The telomeric DNA can be placed on DNA origami nanostructures to make the folding process extremely selective for K+ ions even in the presence of high Na+ concentrations. Here, we demonstrate that the K+-selective G-quadruplex formation is reversible when using a cryptand to remove K+ from the G-quadruplex. We present a full characterization of the reversible switching between single-stranded telomeric DNA and G-quadruplex structures using Forster resonance energy transfer (FRET) between the dyes fluorescein (FAM) and cyanine3 (Cy3). When attached to the DNA origami platform, the G-quadruplex switch can be incorporated into more complex photonic networks, which is demonstrated for a three-color and a four-color FRET cascade from FAM over Cy3 and Cy5 to IRDye700 with G-quadruplex-Cy3 acting as a switchable transmitter.
Author details: | Lydia OlejkoORCiDGND, P. J. Cywinski, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1039/c6nr00119j |
ISSN: | 2040-3364 |
ISSN: | 2040-3372 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/27138897 |
Title of parent work (English): | Nanoscale |
Publisher: | Royal Society of Chemistry |
Place of publishing: | Cambridge |
Publication type: | Article |
Language: | English |
Year of first publication: | 2016 |
Publication year: | 2016 |
Release date: | 2020/03/22 |
Volume: | 8 |
Number of pages: | 9 |
First page: | 10339 |
Last Page: | 10347 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG); Marie Curie FP7 Integration Grant within the 7th European Union Framework Programme; University of Potsdam; Federal Institute for Materials Research (BAM); DFG project [GSC 1013] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |