Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application
- Applications based on Forster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.
| Verfasserangaben: | Niko HildebrandtGND, Daniel Geissler |
|---|---|
| DOI: | https://doi.org/10.1007/978-94-007-2555-3_8 |
| ISBN: | 978-94-007-2554-6 |
| ISBN: | 978-94-007-2555-3 |
| ISSN: | 0065-2598 |
| Titel des übergeordneten Werks (Englisch): | Advances in Experimental Medicine and Biology |
| Verlag: | Springer |
| Verlagsort: | Dordrecht |
| Herausgeber*in(nen): | E Zahavy, A Ordentlich, S Yitzhaki, A Shafferman |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2012 |
| Erscheinungsjahr: | 2012 |
| Datum der Freischaltung: | 26.03.2017 |
| Freies Schlagwort / Tag: | Diagnostics; FRET; Imaging; Quantum dots; Terbium |
| Band: | 733 |
| Seitenanzahl: | 12 |
| Erste Seite: | 75 |
| Letzte Seite: | 86 |
| Fördernde Institution: | European Commission [NANOGNOSTICS-HEALTH-F5-2009-242264] |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Peer Review: | Referiert |
