Effects of extracts prepared from modified porous poly(ether imide) microparticulate absorbers on cytotoxicity, macrophage differentiation and proinflammatory behavior of human monocytic (THP-1) cells
- Remaining uremic toxins in the blood of chronic renal failure patients represent one central challenge in hemodialysis therapies. Highly porous poly(ether imide) (PEI) microparticles have been recently introduced as candidate absorber materials, which show a high absorption capacity for uremic toxins and allow hydrophilic surface modification suitable for minimization of serum protein absorption. In this work, the effects of extracts prepared from PEI microparticles modified by nucleophilic reaction with low molecular weight polyethylene imine (Pei) or potassium hydroxide (KOH), on human monocytic (THP-1) cells are studied. The obtained results suggested that the extracts of Pei and KOH modified PEI absorbers have no negative effect on THP-1 cell viability and do not initiate the critical differentiation towards macrophages. The extracts did not enhance transcript or protein levels of investigated proinflammatory markers in THP-1 cells, namely, TNF alpha, MCP1, IL6 and IL8. Based on these findings such modified PEI microparticlesRemaining uremic toxins in the blood of chronic renal failure patients represent one central challenge in hemodialysis therapies. Highly porous poly(ether imide) (PEI) microparticles have been recently introduced as candidate absorber materials, which show a high absorption capacity for uremic toxins and allow hydrophilic surface modification suitable for minimization of serum protein absorption. In this work, the effects of extracts prepared from PEI microparticles modified by nucleophilic reaction with low molecular weight polyethylene imine (Pei) or potassium hydroxide (KOH), on human monocytic (THP-1) cells are studied. The obtained results suggested that the extracts of Pei and KOH modified PEI absorbers have no negative effect on THP-1 cell viability and do not initiate the critical differentiation towards macrophages. The extracts did not enhance transcript or protein levels of investigated proinflammatory markers in THP-1 cells, namely, TNF alpha, MCP1, IL6 and IL8. Based on these findings such modified PEI microparticles should be qualified for further pre-clinical evaluation i.e. in an in vivo animal experiment.…
| Author details: | Reddi K. Kumar, Matthias HeuchelORCiDGND, Karl KratzORCiD, Andreas LendleinORCiDGND, Joachim Jankowski, Sarada D. Tetali |
|---|---|
| DOI: | https://doi.org/10.3233/CH-189112 |
| ISSN: | 1386-0291 |
| ISSN: | 1875-8622 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29630537 |
| Title of parent work (English): | Clinical hemorheology and microcirculation : blood flow and vessels |
| Publisher: | IOS Press |
| Place of publishing: | Amsterdam |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2018/05/04 |
| Publication year: | 2018 |
| Release date: | 2022/03/25 |
| Tag: | Chronic kidney disease; Inflammation; Porous poly(ether imide) microparticulate absorbers; THP-1 cells; Uremic toxins; hemodialysis |
| Volume: | 69 |
| Issue: | 1-2 |
| Number of pages: | 11 |
| First page: | 175 |
| Last Page: | 185 |
| Funding institution: | IGSTC [IGSTC/NPORE/SDT/2012]; Indo-German Science and Technology Centre [IGSTC/NPORE/SDT/2012]; German Federal Ministry for Education and Research (BMBF)Federal Ministry of Education & Research (BMBF) [01DQ13006A, 01DQ13006B, 01DQ13006C]; UGC-SAPUniversity Grants Commission, India; DBT-CREBBDepartment of Biotechnology (DBT) India; DST-FISTDepartment of Science & Technology (India); UPE |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
