Using DNA origami nanostructures to determine absolute cross sections for UV photon-induced DNA strand breakage
- We have characterized ultraviolet (UV) photon-induced DNA strand break processes by determination of absolute cross sections for photoabsorption and for sequence-specific DNA single strand breakage induced by photons in an energy range from 6.50 to 8.94 eV. These represent the lowest-energy photons able to induce DNA strand breaks. Oligonudeotide targets are immobilized on a UV transparent substrate in controlled quantities through attachment to DNA origami templates. Photon-induced dissociation of single DNA strands is visualized and quantified using atomic force microscopy. The obtained quantum yields for strand breakage vary between 0.06 and 0.5, indicating highly efficient DNA strand breakage by UV photons, which is clearly dependent on the photon energy. Above the ionization threshold strand breakage becomes clearly the dominant form of DNA radiation damage, which is then also dependent on the nucleotide sequence.
Author details: | Stefanie VogelORCiDGND, Jenny RackwitzGND, Robin Schuerman, Julia PrinzORCiDGND, Aleksandar R. Milosavljevic, Matthieu Refregiers, Alexandre Giuliani, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1021/acs.jpclett.5b02238 |
ISSN: | 1948-7185 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26536162 |
Title of parent work (English): | The journal of physical chemistry letters |
Publisher: | American Chemical Society |
Place of publishing: | Washington |
Publication type: | Article |
Language: | English |
Year of first publication: | 2015 |
Publication year: | 2015 |
Release date: | 2017/03/27 |
Volume: | 6 |
Issue: | 22 |
Number of pages: | 5 |
First page: | 4589 |
Last Page: | 4593 |
Funding institution: | Marie Curie FP7 Integration Grant within the seventh European Union Framework Programme; Deutsche Forschungsgemeinschaft (DFG); synchrotron SOLEIL [20131271]; German Academic Exchange Service (DAAD) - German Federal Ministry of Education and Research [57055766]; MESTD of RS [171020]; COST Action [MP1002] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |