Detection of dsDNA with [1,3]Dioxolo[4,5-f]benzodioxol (DBD) Dyes
- DBD fluorescent dyes have proven to be useful in numerous applications. To widen the range of biological applications, we propose three different types of DBD molecules that have been modified in such a way that DNA interaction becomes probable. After the successful synthesis of all three compounds, we tested their fluorescent properties and their DNA binding abilities. Two of the three probes exhibit an interaction with dsDNA with subsequent fluorescence enhancement. The determined binding constants of the two new DNA dyes are comparable to other minorgroove-binding dyes. Their large Stokes shifts and their long fluorescent lifetimes are outstanding features of these dyes.
| Author details: | Dörthe Büchner, Leonard JohnORCiDGND, Monique MertensGND, Pablo WessigORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1002/chem.201804057 |
| ISSN: | 0947-6539 |
| ISSN: | 1521-3765 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30102000 |
| Title of parent work (English): | Chemistry - a European journal |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2018/08/13 |
| Publication year: | 2018 |
| Release date: | 2021/07/26 |
| Tag: | DNA recognition; dyes/pigments; fluorescent probes; heterocycles; scatchard plot |
| Volume: | 24 |
| Issue: | 60 |
| Number of pages: | 8 |
| First page: | 16183 |
| Last Page: | 16190 |
| Funding institution: | Bundesministerium fur Wirtschaft und Energie |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
