Tailormade polysaccharides with defined branching patterns
- The heterogeneous nature of non-cellulosic polysaccharides, such as arabinoxylan, makes it difficult to correlate molecular structure with macroscopic properties. To study the impact of specific structural features of the polysaccharides on crystallinity or affinity to other cell wall components, collections of polysaccharides with defined repeating units are required. Herein, a chemoenzymatic approach to artificial arabinoxylan polysaccharides with systematically altered branching patterns is described. The polysaccharides were obtained by glycosynthase-catalyzed polymerization of glycosyl fluorides derived from arabinoxylan oligosaccharides. X-ray diffraction and adsorption experiments on cellulosic surfaces revealed that the physicochemical properties of the synthetic polysaccharides strongly depend on the specific nature of their substitution patterns. The artificial polysaccharides allow structure-property relationship studies that are not accessible by other means.
Author details: | Deborah Senf, Colin RuprechtGND, Saina Kishani, Aleksandar MaticGND, Guillermo Toriz, Paul Gatenholm, Lars Wagberg, Fabian PfrengleORCiD |
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DOI: | https://doi.org/10.1002/anie.201806871 |
ISSN: | 1433-7851 |
ISSN: | 1521-3773 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30044516 |
Title of parent work (English): | Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition |
Subtitle (English): | Enzymatic polymerization of arabinoxylan oligosaccharides |
Publisher: | Wiley-VCH |
Place of publishing: | Weinheim |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/07/25 |
Publication year: | 2018 |
Release date: | 2021/10/04 |
Tag: | carbohydrates; enzymes; glycosynthases; structure elucidation; synthetic methods |
Volume: | 57 |
Issue: | 37 |
Number of pages: | 6 |
First page: | 11987 |
Last Page: | 11992 |
Funding institution: | Max Planck SocietyMax Planck Society; German Research Foundation (DFG, Emmy Noether program)German Research Foundation (DFG) [PF850/1-1, SFB 765]; Knut and Alice Wallenberg FoundationKnut & Alice Wallenberg Foundation |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access / Green Open-Access |