A Highly K+-Selective Two-Photon Fluorescent Probe
- A highly K+-selective two-photon fluorescent probe for the in vitro monitoring of physiological K+ levels in the range of 1-100 mM is reported. The two-photon excited fluorescence (TPEF) probe shows a fluorescence enhancement (FE) by a factor of about three in the presence of 160 mM K+, independently of one-photon (OP, 430 nm) or two-photon (TP, 860 nm) excitation and comparable K+-induced FEs in the presence of competitive Na+ ions. The estimated dissociation constant (K-d) values in Na+-free solutions (K-d(OP)=(28 +/- 5) mM and K-d(TP)=(36 +/- 6) mM) and in combined K+/Na+ solutions (K-d(OP)=(38 +/- 8) mM and K-d(TP)=(46 +/- 25) mM) reflecting the high K+/Na+ selectivity of the fluorescent probe. The TP absorption cross-section (sigma(2PA)) of the TPEF probe+160 mMK(+) is 26 GM at 860 nm. Therefore, the TPEF probe is a suitable tool for the in vitro determination of K+.
Author details: | Thomas SchwarzeORCiD, Janine RiemerORCiDGND, Sascha EidnerORCiD, Hans-Jürgen HoldtORCiD |
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DOI: | https://doi.org/10.1002/chem.201501473 |
ISSN: | 0947-6539 |
ISSN: | 1521-3765 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26178764 |
Title of parent work (English): | Chemistry - a European journal |
Publisher: | Wiley-VCH |
Place of publishing: | Weinheim |
Publication type: | Article |
Language: | English |
Year of first publication: | 2015 |
Publication year: | 2015 |
Release date: | 2017/03/27 |
Tag: | click chemistry; fluorescence; fluorescent probes; potassium; two-photon |
Volume: | 21 |
Issue: | 32 |
Number of pages: | 5 |
First page: | 11306 |
Last Page: | 11310 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |