IR-MALDI ion mobility spectrometry: physical source characterization and application as HPLC detector
- Infrared matrix-assisted laser dispersion and ionization (IR-MALDI) in combination with ion mobility (IM) spectrometry enables the direct analysis of biomolecules in aqueous solution. The release of ions directly from an aqueous solution is based on a phase explosion, induced by the absorption of an IR laser pulse, which disperses the liquid as vapor, nano-and micro-droplets. The ionization process is characterized initially by a broad spatial distribution of the ions, which is a result of complex fluid dynamics and desolvation kinetics. These processes have a profound effect on the shape and width of the peaks in the IM spectra. In this work, the transport of ions by the phase explosion-induced shockwave could be studied independently from the transport by the electric field. The shockwave-induced mean velocities of the ions at different time scales were determined through IM spectrometry and shadowgraphy. The results show a deceleration of the ions from 118 m.s(-1) at a distance of 400 mu m from the liquid surface to 7.1 m.s(-1) atInfrared matrix-assisted laser dispersion and ionization (IR-MALDI) in combination with ion mobility (IM) spectrometry enables the direct analysis of biomolecules in aqueous solution. The release of ions directly from an aqueous solution is based on a phase explosion, induced by the absorption of an IR laser pulse, which disperses the liquid as vapor, nano-and micro-droplets. The ionization process is characterized initially by a broad spatial distribution of the ions, which is a result of complex fluid dynamics and desolvation kinetics. These processes have a profound effect on the shape and width of the peaks in the IM spectra. In this work, the transport of ions by the phase explosion-induced shockwave could be studied independently from the transport by the electric field. The shockwave-induced mean velocities of the ions at different time scales were determined through IM spectrometry and shadowgraphy. The results show a deceleration of the ions from 118 m.s(-1) at a distance of 400 mu m from the liquid surface to 7.1 m.s(-1) at a distance of 10 mm, which is caused by a pile-up effect. Furthermore, the desolvation kinetics were investigated and a first-order desolvation constant of 325 +/- 50 s(-1) was obtained. In the second part, the IR-MALDI-IM spectrometer is used as an HPLC detector for the two-dimensional separation of a pesticide mixture.…
Verfasserangaben: | José Andrés VillatoroORCiDGND, Martin ZühlkeGND, Daniel RiebeORCiDGND, Toralf BeitzORCiD, Marcus Weber, Jens Riedel, Hans-Gerd LöhmannsröbenORCiDGND |
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DOI: | https://doi.org/10.1007/s12127-016-0208-1 |
ISSN: | 1435-6163 |
ISSN: | 1865-4584 |
Titel des übergeordneten Werks (Englisch): | International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry |
Verlag: | Springer |
Verlagsort: | Heidelberg |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
Erscheinungsjahr: | 2016 |
Datum der Freischaltung: | 22.03.2020 |
Freies Schlagwort / Tag: | HPLC; IR-MALDI; Imaging; Ion mobility spectrometry; Laser; Shadowgraphy |
Band: | 19 |
Seitenanzahl: | 11 |
Erste Seite: | 197 |
Letzte Seite: | 207 |
Fördernde Institution: | German Excellence Initiative (DFG - Deutsche Forschungsgemeinschaft); School of Analytical Sciences Adlershof (SALSA); Bundesanstalt fur Materialforschung und-prufung (BAM) |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer Review: | Referiert |