DNA polyplexes with dendritic glycopolymer-entrapped gold nanoparticles

Polyplexes, composed of Salmon DNA and very small gold nanoparticles embedded into a dendritic glycopolymer architecture of sugar-modified poly(ethyleneimine) (PEI-Mal) with a molar mass of about 25,000 g/mol, were characterized by dynamic light scattering (DLS), zeta potential measurements, micro differential scanning calorimetry (mu-DSC) and transmission electron microscopy (TEM). The PEI-Mal-entrapped gold nanoparticles of about 2 nm in diameter influence the polyplex formation of the hyperbranched PEI containing bulky maltose, and in consequence the DNA is more compactized in the inner part of spherical polyplex particles of about 150 nm in diameter. The resulting more compact core shell polyplex particles with embedded gold nanoparticles in the outer polymer shell will be used as components in forthcoming gene delivery experiments. (C) 2017 Elsevier B.V. All rights reserved.

Metadaten
Author details:	Jens Rumschoettel, Sabine Kosmella GND, Claudia Christina Prietzel GND, Dietmar Appelhans, Joachim Koetz ORCiD GND
DOI:	https://doi.org/10.1016/j.colsurfb.2017.03.001
ISSN:	0927-7765
ISSN:	1873-4367
Pubmed ID:	https://pubmed.ncbi.nlm.nih.gov/28319816
Title of parent work (English):	Colloids and surfaces : an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin ; B, Biointerfaces
Publisher:	Elsevier
Place of publishing:	Amsterdam
Publication type:	Article
Language:	English
Year of first publication:	2017
Publication year:	2017
Release date:	2020/04/20
Tag:	DNA polyplexes; Gold nanoparticles; Maltose-modified poly(ethyleneimine); TEM; mu-DSC
Volume:	154
Number of pages:	8
First page:	74
Last Page:	81
Funding institution:	Polymer Science Program; German Research Foundation [INST 336/64-1]
Organizational units:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer review:	Referiert