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Enzyme-Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One-Pot Cascade Reactions
- Despite the rapid development of Pickering interfacial catalysis (PIC) at liquid-liquid interfaces with chemocatalysts, the use of unstable biocatalysts at emulsion interfaces remains a technical challenge. Herein, we present a Pickering interfacial biocatalysis (PIB) platform based on robust and recyclable enzyme-polymer conjugates that act as both catalytic sites and stabilizers at the interface of Pickering emulsions. The conjugates were prepared by growing poly(N-isopropylacrylamide) on a fragile enzyme, benzaldehyde lyase, under physiological conditions. The mild in situ conjugation process preserved the enzyme structure, and the conjugates were used to emulsify a water-organic two-phase system into a stable Pickering emulsion, leading to a significantly larger interfacial area and a 270-fold improvement in catalytic performance as compared to the unemulsified two-phase system. The PIB system could be reused multiple times. Conjugates of other enzymes were also fabricated and applied for cascade reactions.
Verfasserangaben: | Zhiyong Sun, Ulrich GlebeORCiDGND, Himanshu CharanORCiDGND, Alexander BökerORCiDGND, Changzhu WuORCiD |
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DOI: | https://doi.org/10.1002/anie.201806049 |
ISSN: | 1433-7851 |
ISSN: | 1521-3773 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30141281 |
Titel des übergeordneten Werks (Englisch): | Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 23.08.2018 |
Erscheinungsjahr: | 2018 |
Datum der Freischaltung: | 01.09.2021 |
Freies Schlagwort / Tag: | Pickering interfacial catalysis; biphasic catalysis; cascade reactions; enzyme catalysis; enzyme-polymer conjugates |
Band: | 57 |
Ausgabe: | 42 |
Seitenanzahl: | 5 |
Erste Seite: | 13810 |
Letzte Seite: | 13814 |
Fördernde Institution: | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [WU 814/1-1]; China Scholarship Council (CSC)China Scholarship Council |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer Review: | Referiert |