Olga Koshkina, Dana Westmeier, Thomas Lang, Christoph Bantz, Angelina Hahlbrock, Christian Würth, Ute Resch-Genger, Ulrike Braun, Raphael Thiermann, Christoph Weise, Murat Eravci, Benjamin Mohr, Helmut Schlaad, Roland H. Stauber, Dominic Docter, Annabelle Bertin, Michael Maskos
- Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-likeDue to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.…
MetadatenAuthor details: | Olga Koshkina, Dana Westmeier, Thomas Lang, Christoph Bantz, Angelina Hahlbrock, Christian Würth, Ute Resch-Genger, Ulrike Braun, Raphael Thiermann, Christoph Weise, Murat Eravci, Benjamin Mohr, Helmut SchlaadORCiDGND, Roland H. Stauber, Dominic Docter, Annabelle BertinORCiD, Michael Maskos |
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DOI: | https://doi.org/10.1002/mabi.201600074 |
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ISSN: | 1616-5187 |
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ISSN: | 1616-5195 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/27281039 |
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Title of parent work (English): | Macromolecular bioscience |
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Publisher: | Wiley-VCH |
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Place of publishing: | Weinheim |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2016 |
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Publication year: | 2016 |
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Release date: | 2020/03/22 |
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Tag: | cellular uptake; nanoparticles; poly(2-ethyl-2oxazoline); poly(ethylene glycol); protein adsorption |
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Volume: | 16 |
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Number of pages: | 14 |
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First page: | 1287 |
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Last Page: | 1300 |
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Funding institution: | Deutsche Forschungsgemeinschaft (DFG); DFG [SPP1313]; BiomaTiCS; PTE-foundation; Fonds der Chemischen Industrie |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
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Peer review: | Referiert |
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