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Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
(2012)
Lamins build the nuclear lamina and are required for chromatin organization, gene expression, cell cycle progression, and mechanical stabilization. Despite these universal functions, lamins have so far been found only in metazoans. We have identified protein NE81 in Dictyostelium, which has properties that justify its denomination as a lamin-like protein in a lower eukaryote. This is based on its primary structure, subcellular localization, and regulation during mitosis, and its requirement of the C-terminal CaaX box as a posttranslational processing signal for proper localization. Our knockout and overexpression mutants revealed an important role for NE81 in nuclear integrity, chromatin organization, and mechanical stability of cells. All our results are in agreement with a role for NE81 in formation of a nuclear lamina. This function is corroborated by localization of Dictyostelium NE81 at the nuclear envelope in human cells. The discovery of a lamin-like protein in a unicellular organism is not only intriguing in light of evolution, it may also provide a simple experimental platform for studies of the molecular basis of laminopathies.
Inhalt:
- Kurt-R. Biermann und Ingo Schwarz: Der Aachener Kongreß und das Scheitern der indischen Reisepläne Alexander von Humboldts
- Kurt-R. Biermann und Ingo Schwarz: „Sibirien beginnt in der Hasenheide“ – Alexander von Humboldts Neigung zur Moquerie
- Kurt-R. Biermann und Ingo Schwarz: Rezepte des jungen Alexander von Humboldt von 1789 gegen Mangel an Arbeit und an Subsistenz
- Francisco Díaz Solar y Duanel Díaz Infante: Gastón Baquero invita a leer Cosmos, de Alejandro de Humboldt
- Ottmar Ette: The Scientist as Weltbürger: Alexander von Humboldt and the Beginning of Cosmopolitics
- Margot Faak: Alexander von Humboldt. Reise durch Venezuela
- Heinz Krumpel: Zur Aneignung und Verwandlung der Ideen Humboldts und Krauses in Lateinamerika – Gemeinsamkeiten und Unterschiede
- Oliver Lubrich: En el reino de la ambivalencia. La Cuba de Alejandro de Humboldt
- Ursula Thiemer-Sachse: Welche Kunst es ist, Kunst zu begreifen …
Alexander von Humboldts Sicht auf indianische Kunst
- Engelhard Weigl: Alexander von Humboldt and the Beginning of the Environmental Movement
Inhalt:
- Ottmar Ette: Amerika in Asien. Alexander von Humboldts Asie centrale und die russisch-sibirische Forschungsreise im transarealen Kontext
- Eberhard Knobloch und Herbert Pieper: Die Fußnote ber Geognosia in Humboldts Florae Fribergensis specimen
- Heinz Krumpel: Identität und Differenz - Goethes Faust und Alexander von Humboldt
- Ingo Schwarz: "Humbug und Taktlosigkeit" oder "ein anlockendes Aushängeschild". Alexander von Humboldt als Held einer Novelle - 1858
- Ursula Thiemer-Sachse: La recepción en Europa de la obra de Carlos Nebel "Voyage pittoresque et archéologique dans la parte plus intéressante du Mexique"
- Kurt-R. Biermann und Ingo Schwarz: Indianische Reisebegleiter Alexander von Humboldts in Amerika
- Kurt-R. Biermann y Ingo Schwarz: Los compañeros de viaje indios de Alejandro de Humboldt en América
Aus dem Inhalt:
- 10 Jahre Responsibility to Protect: Ein Sieg für die Menschenrechte? – Eine politik- und rechtswissenschaftliche Analyse
- Neue Regeln zur Abwesenheit des Angeklagten vor dem IStGH:
Menschenrechtliche Anforderungen an In-absentia-Verfahren
- EGMR: S.A.S. ./. Frankreich – Urteilsbesprechung zum Burkaverbot
This study aimed to build on the relationship of well-established self-report and behavioral assessments to the latent constructs positive (PVS) and negative valence systems (NVS), cognitive systems (CS), and social processes (SP) of the Research Domain Criteria (RDoC) framework in a large transnosological population which cuts across DSM/ICD-10 disorder criteria categories. One thousand four hundred and thirty one participants (42.1% suffering from anxiety/fear-related, 18.2% from depressive, 7.9% from schizophrenia spectrum, 7.5% from bipolar, 3.4% from autism spectrum, 2.2% from other disorders, 18.4% healthy controls, and 0.2% with no diagnosis specified) recruited in studies within the German research network for mental disorders for the Phenotypic, Diagnostic and Clinical Domain Assessment Network Germany (PD-CAN) were examined with a Mini-RDoC-Assessment including behavioral and self-report measures. The respective data was analyzed with confirmatory factor analysis (CFA) to delineate the underlying latent RDoC-structure. A revised four-factor model reflecting the core domains positive and negative valence systems as well as cognitive systems and social processes showed a good fit across this sample and showed significantly better fit compared to a one factor solution. The connections between the domains PVS, NVS and SP could be substantiated, indicating a universal latent structure spanning across known nosological entities. This study is the first to give an impression on the latent structure and intercorrelations between four core Research Domain Criteria in a transnosological sample. We emphasize the possibility of using already existing and well validated self-report and behavioral measurements to capture aspects of the latent structure informed by the RDoC matrix.