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Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
SIRT6 is a NAD(+)-dependent deacetylase that modulates chromatin structure and safeguards genomic stability. Until now, SIRT6 has been assigned to the nucleus and only nuclear targets of SIRT6 are known. Here, we demonstrate that in response to stress, C. elegans SIR-2.4 and its mammalian orthologue SIRT6 localize to cytoplasmic stress granules, interact with various stress granule components and induce their assembly. Loss of SIRT6 or inhibition of its catalytic activity in mouse embryonic fibroblasts impairs stress granule formation and delays disassembly during recovery, whereas deficiency of SIR-2.4 diminishes maintenance of P granules and decreases survival of C. elegans under stress conditions. Our findings uncover a novel, evolutionary conserved function of SIRT6 in the maintenance of stress granules in response to stress.
Portal alumni
(2005)
Liebe Leserin, lieber Leser, erforschen, was die Welt im Innersten zusammenhält- das ist für viele Studierende ein Traum. Doch welche Opfer muss man bringen, um ihn zu verwirklichen? Welche Bemfsperspektive hat der Bemf Forscher heute noch? Auch viele Absolventen der Universität Potsdam müssen sich diese Fragen beantworten. Zu welchen Antworten einige dabei gekommen sind und welche Probleme sie zu bewältigen haben, vom Spaß am Forschen und von Zukunftsängsten berichten sie in der Rubrik "Forscherkarrieren". Gelder für die Forschung fließen in Deutschland zu spärlich, verglichen mit anderen führenden Industrienationen. So sind die Bedingungen für Forscher hierzulande nicht die besten. Manchen jungen Wissenschaftler zieht es- mitunter notgedrungen- ins Ausland. Wie Deutschland dadurch seine ZukunftsHihigkeit riskiert, thematisiert der Präsident der Fraunhofer-Gesellschaft, Prof. Dr. Hans-Jörg Bullinger, in der Rubrik "wissenstransfer". Auch die Universität ist kein Garant für eine gesicherte Zukunft in der Forschung. Wer sechs Jahre nach der Promotion den Sprung zur Professur nicht geschafft hat, geht einer ungewissen Zukunft als Privatdozent entgegen. Seit einigen Jahren gibt es neben der Habilitation noch einen zweiten Weg zur Professur- die Juniorprofessur. Auch an der Universität Potsdam gibt es seit 2002 Juniorprofessoren, von denen die ersten jetzt evaluiert wurden. Näheres dazu finden Sie ebenfalls in der Rubrik "wissenstransfer". Wer noch nach einer Finanzierungsmöglichkeit für seine Promotion sucht, findet Tipps in der Rubrik "wegweiser". Die Redaktion wünscht Ihnen viel Vergnügen beim Lesen von Portal alumni und freut sich auf zahlreiche Leserbriefe.
The “HPI Future SOC Lab” is a cooperation of the Hasso Plattner Institute (HPI) and industry partners. Its mission is to enable and promote exchange and interaction between the research community and the industry partners.
The HPI Future SOC Lab provides researchers with free of charge access to a complete infrastructure of state of the art hard and software. This infrastructure includes components, which might be too expensive for an ordinary research environment, such as servers with up to 64 cores and 2 TB main memory. The offerings address researchers particularly from but not limited to the areas of computer science and business information systems. Main areas of research include cloud computing, parallelization, and In-Memory technologies.
This technical report presents results of research projects executed in 2018. Selected projects have presented their results on April 17th and November 14th 2017 at the Future SOC Lab Day events.
Environmental monitoring involves the quantification of microscopic cells and particles such as algae, plant cells, pollen, or fungal spores. Traditional methods using conventional microscopy require expert knowledge, are time-intensive and not well-suited for automated high throughput. Multispectral imaging flow cytometry (MIFC) allows measurement of up to 5000 particles per second from a fluid suspension and can simultaneously capture up to 12 images of every single particle for brightfield and different spectral ranges, with up to 60x magnification. The high throughput of MIFC has high potential for increasing the amount and accuracy of environmental monitoring, such as for plant-pollinator interactions, fossil samples, air, water or food quality that currently rely on manual microscopic methods. Automated recognition of particles and cells is also possible, when MIFC is combined with deep-learning computational techniques. Furthermore, various fluorescence dyes can be used to stain specific parts of the cell to highlight physiological and chemical features including: vitality of pollen or algae, allergen content of individual pollen, surface chemical composition (carbohydrate coating) of cells, DNA- or enzyme-activity staining. Here, we outline the great potential for MIFC in environmental research for a variety of research fields and focal organisms. In addition, we provide best practice recommendations.