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- Alcohol dependence (3)
- LC-MRM-MS (2)
- SDS-PAGE (2)
- mass spectrometry (2)
- peptides markers (2)
- relative quantification (2)
- wheat cultivars (2)
- α-amylase/trypsin inhibitors (2)
- Alcohol expectancy (1)
- Betriebssysteme (1)
The α-amylase/trypsin inhibitors (ATIs) are discussed as being responsible for non-celiac wheat sensitivity (NCWS), besides being known as allergenic components for baker’s asthma. Different approaches for characterization and quantification including proteomics-based methods for wheat ATIs have been documented. In these studies generally the major ATIs have been addressed. The challenge of current study was then to develop a more comprehensive workflow encompassing all reviewed wheat-ATI entries in UniProt database. To substantially test proof of concept, 46 German and Turkish wheat samples were used. Two extractions systems based on chloroform/methanol mixture (CM) and under buffered denaturing conditions were evaluated. Three aspects were optimized, tryptic digestion, chromatographic separation, and targeted tandem mass spectrometric analysis (HPLC-MS/MS). Preliminary characterization with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) documented the purity of the extracted ATIs with CM mixture and the amylase (60–80%)/trypsin (10–20%) inhibition demonstrated the bifunctional activity of ATIs. Thirteen (individual/common) biomarkers were established. Major ATIs (7–34%) were differently represented in samples. Finally, to our knowledge, the proposed HPLC-MS/MS method allowed for the first time so far the analysis of all 14 reviewed wheat ATI entries reported.
The α-amylase/trypsin inhibitors (ATIs) are discussed as being responsible for non-celiac wheat sensitivity (NCWS), besides being known as allergenic components for baker’s asthma. Different approaches for characterization and quantification including proteomics-based methods for wheat ATIs have been documented. In these studies generally the major ATIs have been addressed. The challenge of current study was then to develop a more comprehensive workflow encompassing all reviewed wheat-ATI entries in UniProt database. To substantially test proof of concept, 46 German and Turkish wheat samples were used. Two extractions systems based on chloroform/methanol mixture (CM) and under buffered denaturing conditions were evaluated. Three aspects were optimized, tryptic digestion, chromatographic separation, and targeted tandem mass spectrometric analysis (HPLC-MS/MS). Preliminary characterization with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) documented the purity of the extracted ATIs with CM mixture and the amylase (60–80%)/trypsin (10–20%) inhibition demonstrated the bifunctional activity of ATIs. Thirteen (individual/common) biomarkers were established. Major ATIs (7–34%) were differently represented in samples. Finally, to our knowledge, the proposed HPLC-MS/MS method allowed for the first time so far the analysis of all 14 reviewed wheat ATI entries reported.
Objective: To examine prospectively whether early parental child-rearing behavior is a predictor of cardiometabolic outcome in young adulthood when other potential risk factors are controlled. Metabolic factors associated with increased risk for cardiovascular disease have been found to vary, depending on lifestyle as well as genetic predisposition. Moreover, there is evidence suggesting that environmental conditions, such as stress in pre- and postnatal life, may have a sustained impact on an individual's metabolic risk profile. Methods: Participants were drawn from a prospective, epidemiological, cohort study followed up from birth into young adulthood. Parent interviews and behavioral observations at the age of 3 months were conducted to assess child-rearing practices and mother-infant interaction in the home setting and in the laboratory. In 279 participants, anthropometric characteristics, low-density lipoprotein and high-density lipoprotein cholesterol, apolipoproteins, and triglycerides were recorded at age 19 years. In addition, structured interviews were administered to the young adults to assess indicators of current lifestyle and education. Results: Adverse early-life interaction experiences were significantly associated with lower levels of high- density lipoprotein cholesterol and apolipoprotein A1 in young adulthood. Current lifestyle variables and level of education did not account for this effect, although habitual smoking and alcohol consumption also contributed significantly to cardiometabolic outcomes. Conclusions: These findings suggest that early parental child-rearing behavior may predict health outcome in later life through its impact on metabolic parameters in adulthood.
How to understand species' niches and range dynamics: a demographic research agenda for biogeography
(2012)
Range dynamics causes mismatches between a species geographical distribution and the set of suitable environments in which population growth is positive (the Hutchinsonian niche). This is because sourcesink population dynamics cause species to occupy unsuitable environments, and because environmental change creates non-equilibrium situations in which species may be absent from suitable environments (due to migration limitation) or present in unsuitable environments that were previously suitable (due to time-delayed extinction). Because correlative species distribution models do not account for these processes, they are likely to produce biased niche estimates and biased forecasts of future range dynamics. Recently developed dynamic range models (DRMs) overcome this problem: they statistically estimate both range dynamics and the underlying environmental response of demographic rates from species distribution data. This process-based statistical approach qualitatively advances biogeographical analyses. Yet, the application of DRMs to a broad range of species and study systems requires substantial research efforts in statistical modelling, empirical data collection and ecological theory. Here we review current and potential contributions of these fields to a demographic understanding of niches and range dynamics. Our review serves to formulate a demographic research agenda that entails: (1) advances in incorporating process-based models of demographic responses and range dynamics into a statistical framework, (2) systematic collection of data on temporal changes in distribution and abundance and on the response of demographic rates to environmental variation, and (3) improved theoretical understanding of the scaling of demographic rates and the dynamics of spatially coupled populations. This demographic research agenda is challenging but necessary for improved comprehension and quantification of niches and range dynamics. It also forms the basis for understanding how niches and range dynamics are shaped by evolutionary dynamics and biotic interactions. Ultimately, the demographic research agenda should lead to deeper integration of biogeography with empirical and theoretical ecology.
In today’s life, embedded systems are ubiquitous. But they differ from traditional desktop systems in many aspects – these include predictable timing behavior (real-time), the management of scarce resources (memory, network), reliable communication protocols, energy management, special purpose user-interfaces (headless operation), system configuration, programming languages (to support software/hardware co-design), and modeling techniques. Within this technical report, authors present results from the lecture “Operating Systems for Embedded Computing” that has been offered by the “Operating Systems and Middleware” group at HPI in Winter term 2013/14. Focus of the lecture and accompanying projects was on principles of real-time computing. Students had the chance to gather practical experience with a number of different OSes and applications and present experiences with near-hardware programming. Projects address the entire spectrum, from bare-metal programming to harnessing a real-time OS to exercising the full software/hardware co-design cycle. Three outstanding projects are at the heart of this technical report. Project 1 focuses on the development of a bare-metal operating system for LEGO Mindstorms EV3. While still a toy, it comes with a powerful ARM processor, 64 MB of main memory, standard interfaces, such as Bluetooth and network protocol stacks. EV3 runs a version of 1 1 Introduction Linux. Sources are available from Lego’s web site. However, many devices and their driver software are proprietary and not well documented. Developing a new, bare-metal OS for the EV3 requires an understanding of the EV3 boot process. Since no standard input/output devices are available, initial debugging steps are tedious. After managing these initial steps, the project was able to adapt device drivers for a few Lego devices to an extent that a demonstrator (the Segway application) could be successfully run on the new OS. Project 2 looks at the EV3 from a different angle. The EV3 is running a pretty decent version of Linux- in principle, the RT_PREEMPT patch can turn any Linux system into a real-time OS by modifying the behavior of a number of synchronization constructs at the heart of the OS. Priority inversion is a problem that is solved by protocols such as priority inheritance or priority ceiling. Real-time OSes implement at least one of the protocols. The central idea of the project was the comparison of non-real-time and real-time variants of Linux on the EV3 hardware. A task set that showed effects of priority inversion on standard EV3 Linux would operate flawlessly on the Linux version with the RT_PREEMPT-patch applied. If only patching Lego’s version of Linux was that easy... Project 3 takes the notion of real-time computing more seriously. The application scenario was centered around our Carrera Digital 132 racetrack. Obtaining position information from the track, controlling individual cars, detecting and modifying the Carrera Digital protocol required design and implementation of custom controller hardware. What to implement in hardware, firmware, and what to implement in application software – this was the central question addressed by the project.
Portal Wissen = Exzellenz
(2023)
Was nicht nur gut oder sehr gut ist, nennen wir gern exzellent. Aber was meint das eigentlich? Vom lateinischen „excellere“ kommend, beschreibt es Dinge, Personen oder Handlungen, die „hervor-“ oder „herausragen“ aus der Menge, sich „auszeichnen“ gegenüber anderen. Mehr geht nicht. Exzellenz ist das Mittel der Wahl, wenn es darum geht, der Erste oder Beste zu sein. Und das macht auch vor der Forschung nicht halt. Wer auf die Universität Potsdam schaut, findet zahlreiche ausgezeichnete Forschende, hervorragende Projekte und immer wieder auch aufsehenerregende Erkenntnisse, Veröffentlichungen und Ergebnisse.
Aber ist die UP auch exzellent? Eine Frage, die 2023 ganz sicher andere Wellen schlägt als vielleicht vor 20 Jahren. Denn seit dem Start der Exzellenzinitiative 2005 gelten als – wörtlich – exzellent jene Hochschulen, denen es gelingt, in dem umfangreichsten Förderprogramm für Wissenschaft in Deutschland einen Zuschlag zu erhalten. Egal ob in Form von Graduiertenschulen, Forschungsclustern oder – seit Fortsetzung des Programms ab 2019 unter dem Titel „Exzellenzstrategie“ – ganzen Exzellenzuniversitäten: Wer im Kreis der Forschungsuniversitäten zu den Besten gehören will, braucht das Siegel der Exzellenz. In der gerade eingeläuteten neuen Wettbewerbsrunde der „Exzellenzstrategie des Bundes und der Länder“ bewirbt sich die Universität Potsdam mit drei Clusterskizzen um Förderung.
Ein Antrag kommt aus der Ökologie- und Biodiversitätsforschung. Ziel ist es, ein komplexes Bild ökologischer Prozesse zu zeichnen – und dabei die Rolle von einzelnen Individuen ebenso zu betrachten wie das Zusammenwirken vieler Arten in einem Ökosystem, um die Funktion der Artenvielfalt genauer zu bestimmen. Eine zweite Skizze haben die Kognitionswissenschaften eingereicht. Hier soll das komplexe Nebeneinander von Sprache und Kognition, Entwicklung und Lernen sowie Motivation und Verhalten als dynamisches Miteinander erforscht werden – wobei auch mit den Erziehungswissenschaften kooperiert wird, um verknüpfte Lernund Bildungsprozesse stets mitzudenken. Der dritte Antrag aus den Geo- und Umweltwissenschaften nimmt extreme und besonders folgenschwere Naturgefahren und -prozesse wie Überschwemmungen und Dürren in den Blick. Die Forschenden untersuchen die Extremereignisse mit besonderem Fokus auf deren Wechselwirkung mit der Gesellschaft, um mit ihnen einhergehende Risiken und Schäden besser einschätzen sowie künftig rechtzeitig Maßnahmen einleiten zu können.
„Alle drei Anträge zeichnen ein hervorragendes Bild unserer Leistungsfähigkeit“, betont der Präsident der Universität, Prof. Oliver Günther, Ph.D. „Die Skizzen dokumentieren eindrucksvoll unser Engagement, vorhandene Forschungsexzellenz sowie die Potenziale der Universität Potsdam insgesamt. Allein die Tatsache, dass sich drei schlagkräftige Konsortien in ganz unterschiedlichen Themenbereichen zusammengefunden haben, zeigt, dass wir auf unserem Weg in die Spitzengruppe der deutschen Universitäten einen guten Schritt vorangekommen sind.“
In diesem Heft schauen wir, was sich in und hinter diesen Anträgen verbirgt: Wir haben mit den Wissenschaftlerinnen und Wissenschaftlern gesprochen, die sie geschrieben haben, und sie gefragt, was sie sich vornehmen, sollten sie den Zuschlag erhalten und ein Cluster an die Universität holen. Wir haben aber auch auf die Forschung geschaut, die zu den Anträgen geführt hat und die schon länger das Profil der Universität prägt und ihr national wie international Anerkennung eingebracht hat. Wir stellen eine kleine Auswahl an Projekten, Methoden und Forschenden vor, um zu zeigen, warum in diesen Anträgen tatsächlich exzellente Forschung steckt! Übrigens: Auch „Exzellenz“ ist nicht das Ende der Fahnenstange. Immerhin lässt sich das Adjektiv exzellent sogar steigern. In diesem Sinne wünschen wir exzellentestes Vergnügen beim Lesen!
Portal Wissen = Excellence
(2023)
When something is not just good or very good, we often call it excellent. But what does that really mean? Coming from the Latin word “excellere,” it describes things, persons, or actions that are outstanding or superior and distinguish themselves from others. It cannot get any better. Excellence is the top choice for being the first or the best. Research is no exception.
At the university, you will find numerous exceptional researchers, outstanding projects, and, time and again, sensational findings, publications, and results. But is the University of Potsdam also excellent? A question that will certainly create a different stir in 2023 than it did perhaps 20 years ago. Since the launch of the Excellence Initiative in 2005, universities that succeed in winning the most comprehensive funding program for research in Germany have been considered – literally – excellent. Whether in the form of graduate schools, research clusters, or – since the program was continued in 2019 under the title “Excellence Strategy” – entire universities of excellence: Anyone who wants to be among the best research universities needs the seal of excellence.
The University of Potsdam is applying for funding with three cluster proposals in the recently launched new round of the “Excellence Strategy of the German Federal and State Governments.” One proposal comes from ecology and biodiversity research. The aim is to paint a comprehensive picture of ecological processes by examining the role of single individuals as well as the interactions among many species in an ecosystem to precisely determine the function of biodiversity. A second proposal has been submitted by the cognitive sciences. Here, the complex coexistence of language and cognition, development and learning, as well as motivation and behavior will be researched as a dynamic interrelation. The projects will include cooperation with the educational sciences to constantly consider linked learning and educational processes. The third proposal from the geo and environmental sciences concentrates on extreme and particularly devastating natural hazards and processes such as floods and droughts. The researchers examine these extreme events, focusing on their interaction with society, to be able to better assess the risks and damages they might involve and to initiate timely measures in the future.
“All three proposals highlight the excellence of our performance,” emphasizes University President Prof. Oliver Günther, Ph.D. “The outlines impressively document our commitment, existing research excellence, and the potential of the University of Potsdam as a whole. The fact that three powerful consortia have come together in different subject areas shows that we have taken a good step forward on our way to becoming one of the top German universities.”
In this issue, we are looking at what is in and behind these proposals: We talked to the researchers who wrote them. We asked them about their plans in case their proposals are successful and they bring a cluster of excellence to the university. But we also looked at the research that has led to the proposals, has long shaped the university’s profile, and earned it national and international recognition. We present a small selection of projects, methods, and researchers to illustrate why there really is excellent research in these proposals!
By the way, “excellence” is also not the end of the flagpole. After all, the adjective “excellent” even has a comparative and a superlative. With this in mind, I wish you the most excellent pleasure reading this issue!