Refine
Has Fulltext
- no (2)
Year of publication
- 2015 (2) (remove)
Document Type
- Article (1)
- Conference Proceeding (1)
Language
- English (2)
Is part of the Bibliography
- yes (2)
Keywords
- liposomes (1)
- maleimide (1)
- membranes (1)
- palmitic acid (1)
- palmitoylation (1)
- peptides (1)
Institute
This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.