Refine
Year of publication
Document Type
- Article (76)
- Postprint (8)
- Other (6)
- Monograph/Edited Volume (1)
- Conference Proceeding (1)
- Doctoral Thesis (1)
- Preprint (1)
- Review (1)
Is part of the Bibliography
- yes (95)
Keywords
- AC electrokinetics (4)
- RPA (4)
- Dielectrophoresis (3)
- DNA (2)
- DNA sensor (2)
- Escherichia coli (2)
- In vitro translation (2)
- Interdigitated electrodes (2)
- PCR (2)
- Plasmodium falciparum (2)
The progesterone concentration in blood samples can be utilised as a marker for the diagnosis of early pregnancy, endocrinopathy and virilism. Here, we describe a method for progesterone detection and measurement in whole blood samples by a surface sensitive biosensor used in conjunction with an integrated optical grating coupler. This device determines refractive index changes near the biosensor's surface. Hence, biological species bound to a surface layer can be measured in real-time without any label. For the measurements, we have modified the indirect competitive immonoassay principle. The concentration of the progesterone antibody was kept at 1 µg/ml. Progesterone concentration was determined in buffer solution and whole blood in a range between 0.005 and 10 ng/ml. The detection limit was determined to be 3 pM. The relative standard deviation was calculated to be 3.5%.
An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated
Biosensoren
(2003)
Trends in der Bioanalytik
(2002)