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Methodik der funktionellen Genomanalyse : wie mit Mikroarrays die Aktivität vieler Gene erfasst wird
(2002)
We discuss the evolution of white dwarf containing binaries, in particular such systems consisting of a white dwarf and a main sequence star which have the potential to produce a Type Ia supernova. After investigating current problems in connecting observations of supersoft X-ray sources with such systems, we consider two major problems which theoretical models encounter to produce Chandrasekhar-mass white dwarfs: the helium shell burning instability and the white dwarf spin-up. We conclude by suggesting that the formation of Chandrasekhar-mass white dwarfs may be easier when these two problem are considered simultaneously.
Based on NMR spectroscopic information about the allosamidin-hevamine complex, ab initio MO calcns. of the ring current effect of the arom. moieties of Trp255, Tyr183 and Tyr6 of hevamine were carried out to investigate the role of these amino acid residues in binding interactions with allosamidin in soln. In addn., the intermol. steric compression effect on the 13C chem. shifts of the allosamizoline carbon atoms and the hydrogen bonding to Glu127 was identified. It can be inferred that the binding forces are strongest in the allosamizoline moiety of allosamidin.
We present a data set of images of the gravitationally lensed quasar Q2237+0305, that was obtained at the Apache Point Observatory (APO) between June 1995 and January 1998. Although the images were taken under variable, often poor seeing conditions and with coarse pixel sampling, photometry is possible for the two brighter quasar images A and B with the help of exact quasar image positions from HST observations. We obtain a light curve with 73 data points for each of the images A and B. There is evidence for a long (ga 100 day) brightness peak in image A in 1996 with an amplitude of about 0.4 to 0.5 mag (relative to 1995), which indicates that microlensing has been taking place in the lensing galaxy. Image B does not vary much over the course of the observation period. The long, smooth variation of the light curve is similar to the results from the OGLE monitoring of the system (Wozniak et al. cite{Wozniak00}). Based on observations obtained with the Apache Point Observatory 3.5-meter telescope, which is owned and operated by the Astrophysical Research Consortium.
We present the first result of a comprehensive spectroscopic study of quasar host galaxies. On-axis, spatially resolved spectra of low redshift quasars have been obtained with FORS1, mounted on the 8.2 m ESO Very Large Telescope, Antu. The spectra are spatially deconvolved using a spectroscopic version of the ``MCS deconvolution algorithm''. The algorithm decomposes two dimensional spectra into the individual spectra of the central point-like nucleus and of its host galaxy. Applied to HE 1503+0228 at z=0.135 (MB=-23.0), it provides us with the spectrum of the host galaxy between 3600 Å and 8500 Å (rest-frame), at a mean resolving power of 700. The data allow us to measure several of the important Lick indices. The stellar populations and gas ionization state of the host galaxy of HE 1503+0228 are very similar to the ones measured for normal non-AGN galaxies. Dynamical information is also available for the gas and stellar components of the galaxy. Using deconvolution and a deprojection algorithm, velocity curves are derived for emission lines, from the center up to 4arcsec away from the nucleus of the galaxy. Fitting a simple three- components mass model (point mass, spherical halo of dark matter, disk) to the position-velocity diagram, we infer a mass of M(r<1 kpc) = (2.0 +/- 0.3)x 1010 Msun within the central kiloparsec of the galaxy, and a mass integrated over 10 kpc of M(r<10 kpc) = (1.9 +/- 0.3) x 1011 Msun, with an additional 10% error due to the uncertainty on the inclination of the galaxy. This, in combination with the analysis of the stellar populations indicates that the host galaxy of HE 1503+0228, is a normal spiral galaxy. Based on observations made with ANTU/UT1 at ESO-Paranal observatory in Chile (program 65.P-0361(A)), and with the ESO 3.5 m NTT, at La Silla observatory (program 62.P-0643(B)).
Cationic ionenes bearing hydrophobic side chains were synthesized, which behave as micellar polymers of the polysoap type. The hydrophobic chains were either hydrocarbons or fluorocarbons, or a mixture of both, in the form of statistical as well as block copolymers. These amphiphilic polymers were studied and compared with each other and with low molar mass analogous surfactants, especially with respect to their hydrophobic association in aqueous solution. The particular molecular structure of the ionenes synthesized results in polymeric surfactants with high mobility of the fluorocarbon chains. Most noteworthy, the behavior of the hydrocarbon-fluorocarbon block copolymer soaps in aqueous solution indicates microphase separation into hydrocarbon-rich and fluorocarbon-rich hydrophobic domains, thus yielding multicompartment micelles.
Highly relativistic jets are most probably driven by strong magnetic fields and launched from the accretion disk surrounding a central black hole. Applying the jet flow parameters (velocity, density, temperature) calculated from the magnetohydrodynamic (MHD) equations, we derive the thermal X-ray luminosity along the inner jet flow in the energy range 0.2-10.1 keV. Here, we concentrate on the case of Galactic microquasars emitting highly relativistic jets. For a 5 Msun central object and a jet mass flow rate of dot {M}j = 10-8 Msun yr-1 we obtain a jet X-ray luminosity LX ~ 1033 erg s-1. Emission lines of Fe XXV and Fe XXVI are clearly visible. Relativistic effects such as Doppler shift and boosting were considered for different inclinations of the jet axis. Due to the chosen geometry of the MHD jet the inner X-ray emitting part is not yet collimated. Therefore, depending on the viewing angle, the Doppler boosting does not play a major role in the total spectra.
We report on the growth and structure of hybrid clay-based multilayers obtained by electrostatic self-assembly (also known as layer-by-layer assembly) of poly(diallylpyrrolidinium bromide) and a synthetic hectorite (Laponite). By combining ellipsometry, atomic force microscopy, and specular and off-specular grazing angle X-ray scattering measurements, we show that platelets pack in the vertical direction according to a distribution of distances between nearest neighbors of about 3 Å standard deviation. The accumulation of such random fluctuations in the vertical direction results in the loss of layering of the platelets farther than about 75 Å from the substrate. In this respect, most of the film should be considered as a nanocomposite with preferential orientation of the platelets, rather than as a real multilayer. The model is quantitatively supported by simulations of the specular and off-specular scattering of such multilayers.
We present VR observations of QSO 2237+0305 conducted by the Gravitational Lensing International Time Project collaboration from 1999 October 1 to 2000 February 3. The observations were made with the 2.56 m Nordic Optical Telescope at Roque de los Muchachos Observatory, La Palma (Spain). The point-spread function (PSF) fitting method and an adapted version of the ISIS subtraction method have been used to derive the VR light curves of the four components (A-D) of the quasar. The mean errors range in the intervals 0.01-0.04 mag (PSF fitting) and 0.01-0.02 mag (ISIS subtraction), with the faintest component (D) having the largest uncertainties. We address the relatively good agreement between the A and D light curves derived using different filters, photometric techniques, and telescopes. The new VR light curves of component A extend the time coverage of a high-magnification microlensing peak, which was discovered by the OGLE team.
A simple synthetic route to a new poly(diallylammonium) salt functionalized by a styrene group is presented. This reactive polymer was employed for polyelectrolyte multilayer films using electrostatical layer-by-layer self- assembly, together with an inorganic polyanion, namely an exfoliated hectorite clay. To enhance their stability, the final hybrid multilayers were cross-linked by exposure to UV light, leading only to a minor shrinkage. Alternatively, the reactive polycation was cross-linked after each adsorption step. X-ray reflectometry revealed that the two types of films dispose of an internal order with a short length scale, that seems insensitive to the photo-cross-linking. Cross- linking after each adsorption step, however, results in more regular film growth, and reduces the films? roughness and the amount of polyanion deposited. Under these conditions, the films seem to grow by deposition of submonolayers with a combined vertical and lateral expansion, resulting in the self-healing of previously deposited, incomplete layers.
In this article, the synthesis of analogs of N,N',N''-triacetylchitotriose in which the central sugar residue was replaced by a succinic acid is presented. Mol. modeling calcns. revealed that the pseudotrisaccharides exist in low energy extended conformations which show similar space filling as N,N',N''-triacetylchitotriose. Of the N,N',N''-triacetylchitotriose pseudosugar analogs tested as chitinase inhibitors, none showed any appreciable competition (numerical data not presented). The conformational anal. along with further synthetic efforts will hopefully lead to more efficient pseudosaccharides as chitinase inhibitors.
Electrostatically self-assembled (ESA) polyelectrolyte films show in general no internal structure. The use of special polycations, however, namely of lyotropic ionenes, may give rise to highly ordered coatings. In this article, the influence of the charge density of the polyanion, as well as the distribution of the charged groups within this polymer, is examined, using a series of anionic cellulose derivatives. Various techniques were used to study the films? growth and internal structure. Both showed to be affected in particular by the charge density but also by the substitution pattern.
Novel aspects of symbiotic nitrogen fixation uncovered by transcript profiling with cDNA arrays
(2002)
More on nomore
(2002)
A significant seasonal variation in size at settlement has been observed in newly settled larvae of Dreissena polymorpha in Lake Constance. Diet quality, which varies temporally and spatially in freshwater habitats, has been suggested as a significant factor influencing the life history and development of freshwater invertebrates. Accordingly, experiments were conducted with field-collected larvae to test the proposal that diet quality can determine planktonic larval growth rates, size at settlement and subsequent post-metamorphic growth rates. Larvae were fed one of two diets or starved. One diet was composed of cyanobacterial cells, which are de; cient in polyunsaturated fatty acids (PUFAs) and the other was a mixed diet rich in PUFAs. Freshly metamorphosed animals from the starvation treatment had a carbon content per individual 70% lower than that of larvae fed the mixed diet. This apparent exhaustion of larval internal reserves resulted in a 50% reduction of the post-metamorphic growth rates. Growth was also reduced in animals previously fed the cyanobacterial diet. Hence, low food quantity or low food quality during the larval stage of D. polymorpha, lead to irreversible effects for post-metamorphic animals and are related to inferior competitive abilities.
Zusammenfassung Bei der Umsetzung der Wasserrahmenrichtlinie werden es weniger die Bewirtschaftungspläne mit ihren stark aggregierten Zielvorgaben sein die für die Planungspraxis von Bedeutung sind, sondern vielmehr die zu ihrer Realisierung auf lokaler und regionaler Ebene ansetzenden Maßnahmenprogramme. Da es zur Erreichung der von der Wasserrahmenrichtlinie vorgegebenen Qualitätsmerkmale vor allem notwendig sein wird, die diffusen Stoffeinträge in die Gewässer zu reduzieren, werden zahlreiche flächenbezogene Maßnahmen notwendig werden, die an der Landnutzung ansetzen. Um Doppelarbeiten zu vermeiden, bietet es sich an, zu ihrer Ableitung und Begründung, so weit als möglich auf andere raumrelevante Fachplanungen zurückzugreifen. Aufgrund ihres flächendeckenden Ansatzes und zahlreicher Aussagen zu Landschaftsfunktionen und -potenzialen, die auch mit Inhalten der WRRL in Verbindung gebracht werden können, kommen hier vor allem die Möglichkeiten der Landschaftsplanung ins Spiel. Für die Zuweisung räumlicher Vorrang- und Vorbehaltsfunktionen kann auf die Raumordnung und Landesplanung zurückgegriffen werden. Dass die Bewirtschaftungspläne nach der WRRL aufgrund der zwingend umzusetzenden EU-rechtlichen Vorgaben absehbar verbindliche Rahmenbedingungen setzen wird dabei Auswirkungen auf die Struktur der regionalplanerischen Abwägung haben. Gleichermaßen wichtig wie die organisatorischen Aspekte bei der Zusammenführung von Aussagen für verschiedene Teileinzugsgebiete in den Bewirtschaftungsplänen ist es, inhaltlich-methodische Strukturen einer Zusammenarbeit zu entwickeln. Hier treten Fragen auf, wie es möglich sein wird, zwischen den verschiedenen Disziplinen und Fachverwaltungen eine gemeinsame Sprache bzw. geeignete Parameter zu entwickeln, mit denen naturschutzfachliche Entwicklungsziele in eine Form überführt werden können, in der sie von der Wasserwirtschaftsverwaltung verstanden und umgesetzt werden können ("Übersetzungsproblem"). Weiterhin muss nach Möglichkeiten gesucht werden, auf verschiedenen Einzugsgebietsmaßstäben gewonnene Erkenntnisse zueinander bzw. zum Gesamt-Einzugsgebiet in Verbindung zu setzen ("Skalenproblem").
We present the first result of a comprehensive spectroscopic study of quasar host galaxies. On-axis, spatially resolved spectra of low redshift quasars have been obtained with FORS1, mounted on the 8.2 m ESO Very Large Telescope, Antu. The spectra are spatially deconvolved using a spectroscopic version of the ``MCS deconvolution algorithm''. The algorithm decomposes two dimensional spectra into the individual spectra of the central point-like nucleus and of its host galaxy. Applied to HE 1503+0228 at z=0.135 (MB=-23.0), it provides us with the spectrum of the host galaxy between 3600 Å and 8500 Å (rest-frame), at a mean resolving power of 700. The data allow us to measure several of the important Lick indices. The stellar populations and gas ionization state of the host galaxy of HE 1503+0228 are very similar to the ones measured for normal non-AGN galaxies. Dynamical information is also available for the gas and stellar components of the galaxy. Using deconvolution and a deprojection algorithm, velocity curves are derived for emission lines, from the center up to 4arcsec away from the nucleus of the galaxy. Fitting a simple three-components mass model (point mass, spherical halo of dark matter, disk) to the position-velocity diagram, we infer a mass of M(r<1 kpc) = (2.0 +/- 0.3)x 1010 Msun within the central kiloparsec of the galaxy, and a mass integrated over 10 kpc of M(r<10 kpc) = (1.9 +/- 0.3) x 1011 Msun, with an additional 10 % error due to the uncertainty on the inclination of the galaxy. This, in combination with the analysis of the stellar populations indicates that the host galaxy of HE 1503+0228, is a normal spiral galaxy. Based on observations made with ANTU/UT1 at ESO-Paranal observatory in Chile (program 65.P-0361(A)), and with the ESO 3.5 m NTT, at La Silla observatory (program 62.P-0643(B)).
In allen schulischen Bereichen gibt es leistungsschwache Kinder. Benötigt man für den Bereich des Lesen- und Schreibenlernens eine diagnostische Kategorie "Lese-Rechtschreibschwäche? Und ein weiteres Konstrukt "Legasthenie"? Es werden Vorschläge für praxisbezogene diagnostische Kategorien diskutiert.
Für die schulische Arbeit mit lese-rechtschreibschwachen Kindern relevante Themen werden aus der derzeitigen psychologisch-pädagogischer Sicht angesprochen. Dabei geht es um die Darstellung und Diskussion des entwicklungsorientierten Ansatzes, um Prävention, Diagnose und Förderung beim Lesen- und Schreibenlernen.
In dem Band sind Lesarten zu Brigitte Reimanns wichtigsten Buch "Franziska Linkerhand" vereinigt. Es ging um unterschiedliche Ansätze verschiedener Generationen zu diesem Roman. Außerdem enthält der Band einen Beitrag von Regine Kühn über die Versuche, Reimanns Roman bzw. die Tagebücher zu verfilmen.
Negotiating the global and the local : Leslie Marmon Silko's almanac of the dead as 'Glocal Fiction'
(2002)
This essay explores Leslie Marmon Silko's fictional negotiations of conflicting and overlapping discourses from Native American and Euroamerican cultures in the transnational contact spaces of the Americas. The dialogic juxtapositions of a variety of cultural practices, ranging from indigenous prophecies to the writings of Marx, are central aspects of the glocal formations in Silko's Almanac of the Dead. The novel furthermore functions as a cultural representation and projection of globalization-from-below, i.e., a transgressive social movement which counters the effects of neocolonial globalization by a critical inclusion of global and local forms of consciousness and agencies.
Dreierlei will die Autorin mit diesem unkonventionell geschriebenen Buch bei angehenden Pädagogen erreichen: sie verunsichern, ihre Sachkenntnis befördern und sie ermutigen. Im 1. Teil hinterfragt sie einige verbreitete Vorstellungen über den pädagogischen Berufsalltag und beschreibt den Zauber des Berufsanfangs. Teil 2 gibt Handlungsanregungen zu Problemen, die jungen Lehrern zu schaffen machen: knappe Zeit, Wirkung auf Schüler, Methodenmonotonie oder Disziplin der Klasse. Der 3. Teil zeigt u.a., wie sich der Preis für das Durchhalten reduzieren lässt. Damit erhalten Berufsanfänger jene Orientierungshilfe, die ihnen zu mehr Gelassenheit verhilft.
Cafeteria-Systeme
(2002)
Sacrum imperium
(2002)
Oligosaccharides composed of 2-acetamido-2-deoxy-D-glucopyranose (GlcNAc) and/or 2-amino-2-deoxy-D- glucopyranose (GlcN) were prepd. by chem. degrdn. of chitin or chitosan and sepd. by gel permeation chromatog. Oligosaccharides obtained after enzymic hydrolysis of chitosan [FA 0.19] with a fungal chitinase were derivatized by reductive amination with 2-aminoacridone and sequenced by matrix-assisted laser desorption ionization time-of-flight postsource decay (PSD) mass spectrometry (MS). The sequence of a trimer, D1A2, was established as D-A-A. The compn. of a hexamer D3A3 was .apprx.65% D-A-D-D-A-A and 35% D-D-A-D-A-A. The PSD MS of a nonamer D5A4-amac revealed four isobaric species D-X-Y-D-X-Y-D-A-A, where A is GlcNAc, D is GlcN, and X and Y (X ¹ Y) are mutually either D or A. This structure motif was also obsd. in a dodecamer D7A5 which was composed of eight isobaric sequences of the general formula (D-X-Y)3- D-A-A.
Kampf als Daseinsform
(2002)
Das Jahrhundert der Wölfe
(2002)