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[1-14C]-N-Acetyldopamine (NADA) was oxidized in the presence of methyl [3-3H]-β-alanate with mushroom tyrosinase. The complex mixture of reaction products was partly resolved by chromatographic procedures and analyzed by spectroscopic methods. Methyl-β-alanate is incorporated to only a small extent into oxidation products of NADA which inter alia are presumed to be oligomeric hydroxyquinones. After oxidation of [1-14C, 2-3H]-NADA with preparations from tanning Manduca sexta pupal cuticle, N-acetylnoradrenalin was identified as one of the products. Binding of radioactivity to melanin-like material was also observed. These results suggest that oxidation products different from those formulated usually for the crosslinkages between protein amino groups and N-acetyldopaquinone are deposited in darkly brown coloured insect cuticles during sclerotization.
A package of five FORTRAN programs that provides for fast user-controlled analyses of reading eye fixations is described. The package requires the data to be in a fixation format and to be rescaled to screen dimensions. OLDEYE identifies six types of fixations and calculates descriptive statistics on each of them, on their associated saccades, and on their average pupil diameter. CONVRT represents the text as a string of words that can be coded according to experimentally relevant variables. PLTFIX prints fixation durations by letter position and sequence of occurrence. MODDAT is an interactive program for marking parts of the text in which the data quality is below acceptable standards. It also allows the correction of systematic errors due to calibration or drift. MATCH combines the outputs from OLDEYE, CONVRT, and MODDAT and calculates 11 dependent measures for every word. The output of MATCH is suitable for input to conventional multivariate statistical programs.
The present paper presents FORTRAN programs for reducing eye monitor output to fixations and for mapping these fixations to locations in the stimulus space. Flexible parameters of the fixations program allow for determination of the beginning and end of fixations under different resolution criteria and for indicating loss of accurate measurement. The calibration program is based on a rectangular 9-point fixation grid. Each fixation is rescaled within this grid by solving for a quadratic equation. The rescaled values are output in a flexibly determined rectangular coordinate system that is related to the stimulus space, such as character position on the screen. The programs were developed for the 60-Hz Applied Sciences corneal reflection eye monitor, but they may be used with a number of other systems.
Human placenta is surprisingly rich in post-proline dipeptidyl peptidase activity. Among various cell fractions, microsomes have the highest specific activity. A homogeneous enzyme preparation is obtained in a six-step purification procedure. The final preparation appears homogeneous upon dodecyl sulfate electrophoresis, but analytical isoelectric focussing reveals various active bands with isoelectric points in the range of pH 3 - 4. The enzyme is a glycoprotein containing about 30% carbohydrate. Treatment with neuraminidase lowers the isoelectric points but does not reduce the heterogeneity of the band pattern. The subunit molecular weight is 120000 as estimated by dodecyl sulfate electrophoresis, whereas Mr of the native enzyme is > 200000, as can be concluded from gel filtration experiments. The purified dipeptidyl peptidase cleaves various synthetic and natural peptides, including substance P, kentsin, casomorphin and a synthetic renin inhibitor. In general, the specificity of the placenta peptidase is similar to that of post-proline dipeptidyl peptidase from other sources. Phenylalanylprolyl-P-naphthylamide (Km = 0.02 mM, I/ = 92 Ujmg) is the best substrate among various synthetic peptide derivatives. Only peptides with a free N-terminal amino group and proline, hydroxyproline, or alanine in position 2 of the N-terminal sequence are cieaved. However, X-Pro-Pro- . . . structures, e. g. as in bradykinin, are not attacked. 1 mM bis-(6nitrophenyI)phosphate or 1 mM diisopropylfluorophosphate completely inactivate the peptidase within 30 min at 30°C (pH 8). The peptidase is also completely inhibited by 1 mM Zn²⁺ and by other heavy metals.
Just and Carpenter (1980) presented a theory of reading based on eye fixations wherein their "psycholinguistic" variables accounted for 72% of the variance in word gaze durations. This comment raises some statistical and theoretical problems with their use of simultaneous regression analysis of gaze duration measures and with the resulting theory of reading. A major problem was the confounding of perceptual with psycholinguistic factors. New eye fixation data are presented to support these criticisms. Analysis of fixations within words revealed that most gaze duration variance was contributed by number of fixations rather than by fixation duration.
Sinefungin inhibited the S-adenosylmethionine-dependent farnesoic acid methyltransferase in a cell-free system containing a homogenate of corpora allata from female locusts, Locusta migratoria. The enzyme catalyzed the penultimate step of juvenile hormone biosynthesis in the insects. Culturing corpora allata in the presence of sinefungin greatly suppressed juvenile hormone production. The following in vivo effects were visible after injection of the inhibitor: increase in mortality and reduction of total haemolymph protein liter and ovary fresh weight, as well as length of terminal oocytes. Attempts to reverse these effects by topical application of the juvenile hormone analog ZR-515 (methoprene) were only partly successful. Therefore, the in vivo effects may be due to a general inhibition of methyltransferase enzymes in the insect. Sinefungin appeared to be of potential interest as the first representative of a new class of insect growth regulators.
The haemolymph of the adult Colorado potato beetle, Lepinotarsa decemlineata Say, contains a high molecular weight (MW > 200,000) JH-III specific binding protein. The Kd value of the protein for racemic JH-III is 1.3 ± 0.2 × 10−7 M. It has a lower affinity for racemic JH-I and it does not bind JH-III-diol or JH-III-acid. The binding protein does discriminate between the enantiomers of synthetic, racemic JH-III as was determined by stereochemical anaysis of the bound and the free JH-III. Incubation of racemic JH-III with crude haemolymph results in preferential formation of (10S)-JH-III-acid, the unnatural configuration. The JH-esterase present in L. decemlineata haemolymph is not enantioselective. It is concluded that the most important function of the binding protein is that of a specific carrier, protecting the natural hormone against degradation by esterases. The carrier does not protect JH-I as efficiently as the lower homologue.
Criticisms of the integration of psychotherapy-outcome research performed by Smith, Glass, and Miller (1980) are reviewed and answered. An attempt is made to account for the conflicting points of view in this disagreement in terms of certain issues that have engaged philosophers of science in the 20th century. It is hoped that, in passing, something useful is learned about research of many types on psychotherapy.
Dyslexic and normal readers' eye movements were compared while tracking a moving fixation point and in reading. Contrary to previous reports, the dyslexic and normal readers did not differ in their number of saccades, percentage of regressions, or stability of fixations in the tracking task. Thus, defective oculomotor control was not associated with or a causal factor in dyslexia, and the dyslexics' abnormal eye movements in reading must be related to differences in higher cognitive processes. However, individual differences in oculmotor efficiency, independent of reading ability, were found within both the dyslexic and normal groups, and these differences were correlated in reading and tracking tasks.
Contents: I. Introduction II. Word Coding Processes A. Word Recognition B. Orthographic Coding C. Phonological Coding III. Eye Monitor and Reading Task IV. Group Differences V. Dimensions of Individual Differences A. Regressive Fixation Index and Word Recognition B. Regressive Fixation Index and IQ C. Regressive Fixation Index and Saccade Length D. Regressive Fixation Index and Relative Phonological Skill VI. Multiple Regression Models of Individual Differences A. Disabled Readers in the Aloud Condition B. Disabled Readers in the Silent Condition C. Normal Readers in Silent and Aloud Conditions VII. Conclusions
To investigate the relationship between implicit psychological hypotheses and explicit empirical findings, summaries of twenty published studies on attitude-behaviour consistency were presented to a sample of forty-eight psychology undergraduates. Subjects were asked to estimate the percentage of agreement between attitudes and behaviour obtained by each study. Correlations between subjects' covariation judgements and empirically obtained attitude-behaviour consistencies were minimal and nonsignificant. Results are discussed in the light of more recent research on attitudebehaviour relationship.
The development of phonetic codes in memory of 141 pairs of normal and disabled readers from 7.8 to 16.8 years of age was tested with a task adapted from L. S. Mark, D. Shankweiler, I. Y. Liberman, and C. A. Fowler (Memory & Cognition, 1977, 5, 623–629) that measured false-positive errors in recognition memory for foil words which rhymed with words in the memory list versus foil words that did not rhyme. Our younger subjects replicated Mark et al., showing a larger difference between rhyming and nonrhyming false-positive errors for the normal readers. The older disabled readers' phonetic effect was comparable to that of the younger normal readers, suggesting a developmental lag in their use of phonetic coding in memory. Surprisingly, the normal readers' phonetic effect declined with age in the recognition task, but they maintained a significant advantage across age in the auditory WISC-R digit span recall test, and a test of phonological nonword decoding. The normals' decline with age in rhyming confusion may be due to an increase in the precision of their phonetic codes.
Linguistic and psycholinguistic accounts based on the study of English may prove unreliable as guides to sentence processing in even closely related languages. The present study illustrates this claim in a test of sentence interpretation by German-, Italian-, and English-speaking adults. Subjects were presented with simple transitive sentences in which contrasts of (1) word order, (2) agreement, (3) animacy, and (4) stress were systematically varied. For each sentence, subjects were asked to state which of the two nouns was the actor. The results indicated that Americans relied overwhelming on word order, using a first-noun strategy in NVN and a second-noun strategy in VNN and NNV sentences. Germans relied on both agreement and animacy. Italians showed extreme reliance on agreement cues. In both German and Italian, stress played a role in terms of complex interactions with word order and agreement. The findings were interpreted in terms of the “competition model” of Bates and MacWhinney (in H. Winitz (Ed.), Annals of the New York Academy of Sciences Conference on Native and Foreign Language Acquisition. New York: New York Academy of Sciences, 1982) in which cue validity is considered to be the primary determinant of cue strength. According to this model, cues are said to be high in validity when they are also high in applicability and reliability.
Protanopie, deuteranopic and tritanopic neutral points were computed by determining the wavelength of light that produced the same quantal-catch ratio in the photopigments as that produced by a broad-band light of specified color temperature (range: 2 800—6 600 K). The Vos-Walraven primaries were used as photopigment absorption spectra that were screened by varying densities of ocular (0.5—2.5 at 400 nm) and macular (0.0—1.0 at 460 nm) pigmentation. The computations were carried out in 1 nm steps for the wavelength range of 380 to 720 nm. Most of the empirically determined mean, neutral-point loci in the literature were predicted from these computations to within 1—2nm when average ocular and macular pigment densities were used. The neutral-point range associated with the extreme values of the prereceptoral screening pigments was up to 25 nm for protanopes and deuteranopes and up to 13 nm for tritanopes.
The spectral efficiency of blackness induction was measured in three normal trichromatic observers and in one deuteranomalous observer. The psychophysical task was to adjust the radiance of a monochromatic 60–120′ annulus until a 45′ central broadband field just turned black and its contour became indiscriminable from a dark surrounding gap that separated it from the annulus. The reciprocal of the radiance required to induce blackness with annulus wavelengths between 420 and 680 nm was used to define a spectral-efficiency function for the blackness component of the achromatic process. For each observer, the shape of this blackness-sensitivity function agreed with the spectral-efficiency function based on heterochromatic flicker photometry when measured with the same 60–120′ annulus. Both of these functions matched the Commission Internationale de l'Eclairage Vλ function except at short wavelengths. Ancillary measurements showed that the latter difference in sensitivity can be ascribed to nonuniformities of preretinal absorption, since the annular field excluded the central 60′ of the fovea. Thus our evidence indicates that, at least to a good first approximation, induced blackness is inversely related to the spectral-luminosity function. These findings are consistent with a model that separates the achromatic and the chromatic pathways.
EMAN is an eye-movement analysis program that consists of four modules. The first module rescales eye positions to coordinates of the display. The second and third modules reduce data to a fixation format and identify areas of bad measurement by means of iterative passes over the data. In the fourth module iterative algorithms are employed for the identification of line numbers and for achieving congruence between fixations and display.