The Cheb Basin (CZ) is a shallow Neogene intracontinental basin filled with fluvial and lacustrine sediments that is located in the western part of the Eger Rift. The basin is situated in a seismically active area and is characterized by diffuse degassing of mantle-derived CO2 in mofette fields. The Hartousov mofette field shows a daily CO2 flux of 23-97 tons of CO2 released over an area of 0.35 km(2) and a soil gas concentration of up to 100% CO2. The present study aims to explore the geo-bio interactions provoked by the influence of elevated CO2 concentrations on the geochemistry and microbial community of soils and sediments. To sample the strata, two 3-m cores were recovered. One core stems from the center of the degassing structure, whereas the other core was taken 8 m from the ENE and served as an undisturbed reference site. The sites were compared regarding their geochemical features, microbial abundances, and microbial community structures. The mofette site is characterized by a low pH and high TOC/sulfate contents. Striking differences in the microbial community highlight the substantial impact of elevated CO2 concentrations and their associated side effects on microbial processes. The abundance of microbes did not show a typical decrease with depth, indicating that the uprising CO2-rich fluid provides sufficient substrate for chemolithoautotrophic anaerobic microorganisms. Illumine MiSeq sequencing of the 16S rRNA genes and multivariate statistics reveals that the pH strongly influences microbial composition and explains around 38.7% of the variance at the mofette site and 22.4% of the variance between the mofette site and the undisturbed reference site. Accordingly, acidophilic microorganisms (e.g., OTUs assigned to Acidobacteriaceae and Acidithiobacillus) displayed a much higher relative abundance at the mofette site than at the reference site. The microbial community at the mofette site is characterized by a high relative abundance of methanogens and taxa involved in sulfur cycling. The present study provides intriguing insights into microbial life and geo-bio interactions in an active seismic region dominated by emanating mantle-derived CO2-rich fluids, and thereby builds the basis for further studies, e.g., focusing on the functional repertoire of the communities. However, it remains open if the observed patterns can be generalized for different time-points or sites.

PURPOSE. Graves' orbitopathy (GO) is an autoimmune orbital disorder associated with Graves' disease caused by thyrotropin receptor autoantibodies. Orbital fibroblasts (OFs) and CD40 play a key role in disease pathogenesis. The bioactive lipid sphingosine-1-phosphate (S1P) has been implicated in promoting adipogenesis, fibrosis, and inflammation in OFs. We investigated the role of CD40 signaling in inducing S1P activity in orbital inflammation. METHODS. OFs and T cells were derived from GO patients and healthy control (Ctl) persons. S1P abundance in orbital tissues was evaluated by immunofluorescence. OFs were stimulated with CD40 ligand and S1P levels were determined by ELISA. Further, activities of acid sphingomyelinase (ASM), acid ceramidase, and sphingosine kinase were measured by ultraperformance liquid chromatography. Sphingosine and ceramide contents were analyzed by mass spectrometry. Finally, the role for S1P in T-cell attraction was investigated by T-cell migration assays. RESULTS. GO orbital tissue showed elevated amounts of S1P as compared to control samples. Stimulation of CD40 induced S1P expression in GO-derived OFs, while Ctl-OFs remained unaffected. A significant increase of ASM and sphingosine kinase activities, as well as lipid formation, was observed in GO-derived OFs. Migration assay of T cells in the presence of SphK inhibitor revealed that S1P released by GO-OFs attracted T cells for migration. CONCLUSIONS. The results demonstrated that CD40 ligand stimulates GO fibroblast to produce S1P, which is a driving force for T-cell migration. The results support the use of S1P receptor signaling modulators in GO management.

Knowledge of the surface temperature distribution on a comet’s nucleus and its temporal evolution at different timescales is key to constraining its thermophysical properties and understanding the physical processes that take place at and below the surface. Here we report on time-resolved maps of comet 67P/Churyumov–Gerasimenko retrieved on the basis of infrared data acquired by the Visible InfraRed and Thermal Imaging Spectrometer (VIRTIS) onboard the Rosetta orbiter in 2014, over a roughly two-month period in the pre-perihelion phase at heliocentric distances between 3.62 and 3.31 au from the Sun. We find that at a spatial resolution ≤15 m per pixel, the measured temperatures point out the major effect that self-heating, due to the complex shape of the nucleus, has on the diurnal temperature variation. The bilobate nucleus of comet 67P also induces daytime shadowing effects, which result in large thermal gradients. Over longer periods, VIRTIS-derived temperature values reveal seasonal changes driven by decreasing heliocentric distance combined with an increasing abundance of ice within the uppermost centimetre-thick layer, which implies the possibility of having a largely pristine nucleus interior already in the shallow subsurface

The whereabouts of the Balaenoptera borealis holotype, the skeleton of a 1819 stranded specimen, have been unknown since the World War II (WWII). Due to nomenclatural confusion, deficient documentation, and finally WWII bombing, which destroyed predominantly cetacean material in the Museum fib Naturkunde Berlin (MfN), the type skeleton of the sei whale sank into oblivion. Construction activities enabled a recent search and study on the remaining whale material. Here, we provide evidence that the type specimen was not destroyed. On the basis of species-wide and individual characters of the type material such as the shape of cranial elements and the pattern of the maxillary foramina, we show that the skull and mandibles, the vertebral column (except the atlas), and the ribs of the holotype remain intact. Further evidence that these skeletal remains belong to the previously missing holotype is provided by the characteristics of the spine. In addition, we analyzed ancient DNA from bone samples and confirm they are B. borealis, and the occurrence of same mitochondrial haplotypes indicate that the bones belong to the same individual. Additionally, a blue inscription was discovered at the caudal epiphysis of a thoracic vertebra; historical research matched this inscription with the material belonging to the former Anatomical-Zootomical Museum, from which the holotype was once bought.

Two principal groups of processes shape mass fluxes from and into a soil: vertical profile development and lateral soil redistribution. Periods having predominantly progressive soil forming processes (soil profile development) alternate with periods having predominantly regressive processes (erosion). As a result, short‐term soil redistribution – years to decades – can differ substantially from long‐term soil redistribution; i.e. centuries to millennia. However, the quantification of these processes is difficult and consequently their rates are poorly understood. To assess the competing roles of erosion and deposition we determined short‐ and long‐term soil redistribution rates in a formerly glaciated area of the Uckermark, northeast Germany. We compared short‐term erosion or accumulation rates using plutonium‐239 and ‐240 (239+240Pu) and long‐term rates using both in situ and meteoric cosmogenic beryllium‐10 (10Be). Three characteristic process domains have been analysed in detail: a flat landscape position having no erosion/deposition, an erosion‐dominated mid‐slope, and a deposition‐dominated lower‐slope site. We show that the short‐term mass erosion and accumulation rates are about one order of magnitude higher than long‐term redistribution rates. Both, in situ and meteoric 10Be provide comparable results. Depth functions, and therefore not only an average value of the topsoil, give the most meaningful rates. The long‐term soil redistribution rates were in the range of −2.1 t ha‐1 yr‐1 (erosion) and +0.26 t ha‐1 yr‐1 (accumulation) whereas the short‐term erosion rates indicated strong erosion of up to 25 t ha‐1 yr‐1 and accumulation of 7.6 t ha‐1 yr‐1. Our multi‐isotope method identifies periods of erosion and deposition, confirming the ‘time‐split approach’ of distinct different phases (progressive/regressive) in soil evolution. With such an approach, temporally‐changing processes can be disentangled, which allows the identification of both the dimensions of and the increase in soil erosion due to human influence

Sequelae of prematurity triggered by oxidative stress and free radical-mediated tissue damage have coined the term "oxygen radical disease of prematurity". Caffeine, a potent free radical scavenger and adenosine receptor antagonist, reduces rates of brain damage in preterm infants. In the present study, we investigated the effects of caffeine on oxidative stress markers, anti-oxidative response, inflammation, redox-sensitive transcription factors, apoptosis, and extracellular matrix following the induction of hyperoxia in neonatal rats. The brain of a rat pups at postnatal Day 6 (P6) corresponds to that of a human fetal brain at 28-32 weeks gestation and the neonatal rat is an ideal model in which to investigate effects of oxidative stress and neuroprotection of caffeine on the developing brain. Six-day-old Wistar rats were pre-treated with caffeine and exposed to 80% oxygen for 24 and 48 h. Caffeine reduced oxidative stress marker (heme oxygenase-1, lipid peroxidation, hydrogen peroxide, and glutamate-cysteine ligase catalytic subunit (GCLC)), promoted anti-oxidative response (superoxide dismutase, peroxiredoxin 1, and sulfiredoxin 1), down-regulated pro-inflammatory cytokines, modulated redox-sensitive transcription factor expression (Nrf2/Keap1, and NF kappa B), reduced pro-apoptotic effectors (poly (ADP-ribose) polymerase-1 (PARP-1), apoptosis inducing factor (AIF), and caspase-3), and diminished extracellular matrix degeneration (matrix metalloproteinases (MMP) 2, and inhibitor of metalloproteinase (TIMP) 1/2). Our study affirms that caffeine is a pleiotropic neuroprotective drug in the developing brain due to its anti-oxidant, anti-inflammatory, and anti-apoptotic properties.

Ancient evaporite deposits are geological archives of depositional environments characterized by a long‐term negative precipitation balance and bear evidence for global ocean element mass balance calculations. Here, Cretaceous selenite pseudomorphs from western Anatolia (‘Rosetta Marble’) — characterized by their exceptional morphological preservation — and their ‘marine’ geochemical signatures are described and interpreted in a process‐oriented context. These rocks recorded Late Cretaceous high‐pressure/low‐temperature, subduction‐related metamorphism with peak conditions of 1·0 to 1·2 GPa and 300 to 400°C. Metre‐scale, rock‐forming radiating rods, now present as fibrous calcite marble, clearly point to selenitic gypsum as the precursor mineral. Stratigraphic successions are recorded along a reconstructed proximal to distal transect. The cyclical alternation of selenite beds and radiolarian ribbon‐bedded cherts in the distal portions are interpreted as a two type of seawater system. During arid intervals, shallow marine brines cascaded downward into basinal settings and induced precipitation. During more humid times, upwelling‐induced radiolarian blooms caused the deposition of radiolarite facies. Interestingly, there is no comparable depositional setting known from the Cenozoic world. Meta‐selenite geochemical data (δ13C, δ18O and 87Sr/86Sr) plot within the range of reconstructed middle Cretaceous seawater signatures. Possible sources for the 13C‐enriched (mean 2·2‰) values include methanogenesis, gas hydrates and cold seep fluid exhalation. Spatially resolved component‐specific analysis of a rock slab displays isotopic variances between meta‐selenite crystals (mean δ13C 2·2‰) and host matrix (mean δ13C 1·3‰). The Cretaceous evaporite‐pseudomorphs of Anatolia represent a basin wide event coeval with the Aptian evaporites of the Proto‐Atlantic and the pseudomorphs share many attributes, including lateral distribution of 600 km and stratigraphic thickness of 1·5 to 2·0 km, with the evaporites formed during the younger Messinian salinity crisis. The Rosetta Marble of Anatolia may represent the best‐preserved selenite pseudomorphs worldwide and have a clear potential to act as a template for the study of meta‐selenite in deep time.

In Germany, the irrigation sector accounts for only 1% of water use. In recent years, however, this sector has attracted more attention due to the occurrence of severe drought periods. Irrigation scheduling systems could support adaptation strategies but little is known about current providers, performance and users. In this study we aimed to depict the current situation of the existence and functioning of irrigation scheduling systems available in Germany. Six methods were identified and assessed based on direct interviews with end-users and a comparative analysis. The results showed a positive feedback from the users. However, the recommendations were rarely implemented, while only the seasonal irrigation requirement was considered to support actual water abstraction. These results were corroborated by the comparative analysis. Five of the six irrigation scheduling systems estimated the seasonal irrigation amount consistently, while wider differences were found by looking at the irrigation season and at the number of irrigations. Overall, it is found that irrigation support systems are valuable tools for supporting adaptation strategies to fast changes in agro-environmental conditions. However, specific assessments based on real measurements should be considered in order to improve the performance of the systems and provide more consistent support to end-users. (c) 2019 John Wiley & Sons, Ltd.

Natural landscape elements (NLEs) in agricultural landscapes contribute to biodiversity and ecosystem services, but are also regarded as an obstacle for large-scale agricultural production. However, the effects of NLEs on crop yield have rarely been measured. Here, we investigated how different bordering structures, such as agricultural roads, field-to-field borders, forests, hedgerows, and kettle holes, influence agricultural yields. We hypothesized that (a) yield values at field borders differ from mid-field yields and that (b) the extent of this change in yields depends on the bordering structure. We measured winter wheat yields along transects with log-scaled distances from the border into the agricultural field within two intensively managed agricultural landscapes in Germany (2014 near Gottingen, and 2015-2017 in the Uckermark). We observed a yield loss adjacent to every investigated bordering structure of 11%-38% in comparison with mid-field yields. However, depending on the bordering structure, this yield loss disappeared at different distances. While the proximity of kettle holes did not affect yields more than neighboring agricultural fields, woody landscape elements had strong effects on winter wheat yields. Notably, 95% of mid-field yields could already be reached at a distance of 11.3 m from a kettle hole and at a distance of 17.8 m from hedgerows as well as forest borders. Our findings suggest that yield losses are especially relevant directly adjacent to woody landscape elements, but not adjacent to in-field water bodies. This highlights the potential to simultaneously counteract yield losses close to the field border and enhance biodiversity by combining different NLEs in agricultural landscapes such as creating strips of extensive grassland vegetation between woody landscape elements and agricultural fields. In conclusion, our results can be used to quantify ecocompensations to find optimal solutions for the delivery of productive and regulative ecosystem services in heterogeneous agricultural landscapes.

We have developed a three-dimensional (3D) graphene electrode suitable for the immobilization of human sulfite oxidase (hSO), which catalyzes the electrochemical oxidation of sulfite via direct electron transfer (DET). The electrode is fabricated by drop-casting graphene-polyethylenimine (G-P) composites on carbon papers (CPs) precoated with graphene oxide (GO). The negatively charged hSO can be adsorbed electrostatically on the positively charged matrix (G-P) on CP electrodes coated with GO (CPG), with a proper orientation for accelerated DET. Notably, further electrochemical reduction of G-P on CPG electrodes leads to a 9-fold increase of the saturation catalytic current density (j(m)) for sulfite oxidation reaching 24.4 +/- 0.3 mu A to cm(-2), the highest value among reported DET-based hSO bioelectrodes. The increased electron transfer rate plays a dominating role in the enhancement of direct enzymatic current because of the improved electric contact of hSO with the electrode, The optimized hSO bioelectrode shows a significant catalytic rate (k(cat): 25.6 +/- 0.3 s(-1)) and efficiency (k(cat)/K-m: 0.231 +/- 0.003 s(-1) mu M-1) compared to the reported hSO bioelectrodes. The assembly of the hSO bioanode and a commercial platinum biocathode allows the construction of sulfite/O-2 enzymatic biofuel cells (EBFCs) with flowing fuels. The optimized EBFC displays an open-circuit voltage (OCV) of 0.64 +/- 0.01 V and a maximum power density of 61 +/- 6 mu W cm(-2) (122 +/- 12 mW m(-3)) at 30 degrees C, which exceeds the best reported value by more than 6 times.