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- Zeitgenossen (8)
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- acceleration (8)
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- algae (8)
- amerikanische Reisetagebücher (8)
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- degradation (8)
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- dissociative electron attachment (8)
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- inference (8)
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- isomerization (8)
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- quasars: general (8)
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- recombination (8)
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- stars: emission-line, Be (8)
- sustainable development (8)
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- tracking (8)
- trends (8)
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- waves (8)
- wh-questions (8)
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- Öffentliche Verwaltung (8)
- Abrüstung (7)
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- Anisotropy (7)
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- Decision-making (7)
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- Disturbance (7)
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- Emotionen (7)
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- Weiterbildung (7)
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- Zinc (7)
- acquisition (7)
- age of acquisition (7)
- aggregation (7)
- algorithms (7)
- alien species (7)
- alignment (7)
- allostatic load (7)
- amygdala (7)
- antibody (7)
- attitude (7)
- augmented reality (7)
- autonomy (7)
- bicameralism (7)
- binaries: eclipsing (7)
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- change (7)
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- colonialism (7)
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- competence (7)
- complexes (7)
- convection (7)
- conversation analysis (7)
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- data (7)
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- defense (7)
- density (7)
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- developmental dyscalculia (7)
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- discrepancy principle (7)
- disturbance (7)
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- ecosystem functioning (7)
- effectiveness (7)
- energy (7)
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- ensemble Kalman filter (7)
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- extinction (7)
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- extreme rainfall (7)
- fiction (7)
- first passage time (7)
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- floodplain (7)
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- food webs (7)
- function (7)
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- galaxies (7)
- galaxies: dwarf (7)
- galaxies: starburst (7)
- gender differences (7)
- geophysics (7)
- global warming (7)
- graphene (7)
- gravity (7)
- heart (7)
- heterogeneous catalysis (7)
- human behaviour (7)
- human impact (7)
- hydrolysis (7)
- ideology (7)
- imaging (7)
- imaging spectroscopy (7)
- infrared: stars (7)
- instability (7)
- insula (7)
- international law (7)
- interoceptive awareness (7)
- jumping (7)
- kardiologische Rehabilitation (7)
- kinematics (7)
- land-use change (7)
- last glacial maximum (7)
- law (7)
- lipid metabolism (7)
- low-energy electrons (7)
- magnetism (7)
- manganese (7)
- manifolds with singularities (7)
- measurement (7)
- memory retrieval (7)
- metabolic networks (7)
- metaphor (7)
- miRNA (7)
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- minerals (7)
- modern Jewish history (7)
- moment tensor (7)
- muscle (7)
- myocardial infarction (7)
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- nonergodicity (7)
- nucleus (7)
- number (7)
- numerical simulations (7)
- openHPI (7)
- organic matter (7)
- orthography (7)
- oscillations (7)
- oxygen (7)
- pH (7)
- pain (7)
- palaeoecology (7)
- paleoecology (7)
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- parameter estimation (7)
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- physics (7)
- planetary nebulae: general (7)
- planets and satellites: rings (7)
- plant diversity (7)
- plasmonics (7)
- plyometric training (7)
- polyelectrolytes (7)
- porous materials (7)
- predator-prey dynamics (7)
- production (7)
- programming (7)
- pronoun resolution (7)
- prosodic boundary cues (7)
- proteasome (7)
- pseudodifferential operators (7)
- public sector (7)
- pulsars: general (7)
- quality assurance (7)
- reduction (7)
- repair (7)
- retinol (7)
- roughness (7)
- screening (7)
- secular trend (7)
- sediment transport (7)
- serotonin (7)
- shape-memory effect (7)
- silicon (7)
- size (7)
- soccer (7)
- social rejection (7)
- social support (7)
- socioeconomic status (7)
- soil erosion (7)
- solar wind (7)
- sorption (7)
- spelling (7)
- stabile Isotope (7)
- stars: late-type (7)
- structure elucidation (7)
- sunspots (7)
- surfactants (7)
- tVNS (7)
- taste (7)
- taxonomy (7)
- teaching quality (7)
- thermokarst (7)
- thermoresponsiv (7)
- thermoresponsive polymers (7)
- time series (7)
- tomato (7)
- tools (7)
- topography (7)
- treeline (7)
- trunk (7)
- ultrasound (7)
- uncertainty analysis (7)
- unemployment (7)
- uplift (7)
- urbanization (7)
- vesicles (7)
- video analysis (7)
- wh-in-situ (7)
- work (7)
- working memory capacity (7)
- world literature (7)
- youth sports (7)
- Ästhetik (7)
- 1800 (6)
- 3D visualization (6)
- AFM (6)
- Absorption (6)
- Acceleration (6)
- Adhäsion (6)
- Ageing (6)
- Agency (6)
- Alaska (6)
- Anisotropy effect (6)
- Antibiotikaresistenz (6)
- Antikörper (6)
- Antwortmengenprogrammierung (6)
- Aristoteles (6)
- Arsenolipids (6)
- Atomwaffen (6)
- Aufmerksamkeit (6)
- Bakterien (6)
- Bayesian data analysis (6)
- Berühren (6)
- Bewegungsökologie (6)
- Bioelectrocatalysis (6)
- Biogenic amine (6)
- Blockcopolymer (6)
- Boden (6)
- Brief (6)
- C-reactive protein (6)
- Cardiovascular diseases (6)
- Caribbean (6)
- Centrosom (6)
- Chemotaxis (6)
- Chimborazo (6)
- Chloroplast (6)
- Chytridiomycota (6)
- Click chemistry (6)
- Climate Change (6)
- Complexity (6)
- Computational modelling (6)
- Computerspiele (6)
- Copper (6)
- Daniel Kehlmann (6)
- Data assimilation (6)
- Deep learning (6)
- Deutschdidaktik (6)
- Deutschunterricht (6)
- Dielektrophorese (6)
- Digitale Plattformen (6)
- Disarmament (6)
- Diskriminierung (6)
- Diskurs (6)
- Diversity (6)
- Dynamic NMR (6)
- Dynamik (6)
- EEG/ERP (6)
- Effizienz (6)
- Egypt (6)
- Ellenberg indicator values (6)
- Eltern (6)
- Emotions (6)
- Energy (6)
- Entzündung (6)
- Ethics (6)
- Europäische Integration (6)
- Fachdidaktik Latein (6)
- Fachwissen (6)
- Flood (6)
- Flood risk (6)
- Fluorescence spectroscopy (6)
- Fokus (6)
- Fremdsprachenunterricht (6)
- Gammastrahlungsastronomie (6)
- Gemeinsame Außen- und Sicherheitspolitik (6)
- Gene-environment interaction (6)
- Genomics (6)
- Georg Foster (6)
- Geowissenschaften (6)
- Geschmack (6)
- Gesundheit (6)
- H II regions (6)
- HIREC (6)
- Hebrew (6)
- Hertzsprung-Russell and C-M diagrams (6)
- Hochschullehre (6)
- Hodge theory (6)
- Humboldt als Vorbild (6)
- Humboldt, Alexander von (6)
- Hypertension (6)
- ISM: kinematics and dynamics (6)
- Immigration (6)
- Immunoassay (6)
- Information (6)
- Instructional quality (6)
- Insulin (6)
- Insurance (6)
- International Relations (6)
- Kenya (6)
- Klassifikation (6)
- Klimaanpassung (6)
- Klimaschutz (6)
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- Kohlendioxid (6)
- Kompetenzen (6)
- Kosmologie (6)
- Kreativität (6)
- Krise (6)
- Lake Van (6)
- Language acquisition (6)
- Lasers (6)
- Latin (6)
- Lehre (6)
- Levy flights (6)
- Lipide (6)
- Local Group (6)
- Longitudinal (6)
- Luftverschmutzung (6)
- Lysiphlebus fabarum (6)
- Magnetohydrodynamik (6)
- Magnetotellurik (6)
- Mandarin Chinese (6)
- Mannheimer Risikokinderstudie (6)
- Markov processes (6)
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- Measurement (6)
- Menderes Massif (6)
- Mental arithmetic (6)
- Mental disorders (6)
- Meta-analysis (6)
- Metapher (6)
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- Microarray (6)
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- Ontologie (6)
- Optimierung (6)
- Optimization (6)
- Oxygen heterocycles (6)
- P300 (6)
- Paleoclimatology (6)
- Paleolimnology (6)
- Palästina (6)
- Paris Agreement (6)
- Peptid (6)
- Perceptual span (6)
- Perturbation (6)
- Perú (6)
- Phenotypic plasticity (6)
- Philologie (6)
- Phonons (6)
- Physikdidaktik (6)
- Poecilia mexicana (6)
- Population genetics (6)
- Potsdamer Lateintag (6)
- Pragmatik (6)
- Predictors (6)
- Prenatal stress (6)
- Professional development (6)
- Psychologie (6)
- Pyrenees (6)
- Python (6)
- Quasare (6)
- RAFT polymerization (6)
- Raman (6)
- Reflexivität (6)
- Reinforcement learning (6)
- Reisetagebücher (6)
- Rezeption (6)
- Rheology (6)
- Ring current effect (6)
- Romania (6)
- Ruthenium (6)
- SDS-PAGE (6)
- SWIM (6)
- Schadensmodellierung (6)
- School (6)
- Seesedimente (6)
- Selenium (6)
- Self-efficacy (6)
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- Sentinel-1 (6)
- Sexual scripts (6)
- Sexual selection (6)
- Sexualität (6)
- Sibirien (6)
- Software (6)
- Soil erosion (6)
- Solanum tuberosum (6)
- Speech perception (6)
- Sphingosine 1-phosphate (6)
- Stable isotopes (6)
- Sternentwicklung (6)
- Storytelling (6)
- Strafrecht (6)
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- Studium (6)
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- Svalbard (6)
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- Tacitus (6)
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- abstract concepts (6)
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- acid ceramidase (6)
- admixture (6)
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- animal movement (6)
- anthropometry (6)
- antioxidants (6)
- architecture (6)
- arctic (6)
- assembly (6)
- behaviour (6)
- bias (6)
- biosensors (6)
- black holes (6)
- blockchain (6)
- body (6)
- capitalism (6)
- carbon sequestration (6)
- catalogs (6)
- classical reception (6)
- cluster analysis (6)
- collective memory (6)
- community effect (6)
- computer games (6)
- constraints (6)
- contrast (6)
- coping (6)
- copolymers (6)
- corpus study (6)
- corruption (6)
- costs (6)
- crown compounds (6)
- crystal-structure (6)
- cultural diversity (6)
- cultural studies (6)
- cyber victimization (6)
- cyber-physical systems (6)
- cytoskeleton (6)
- databases (6)
- design (6)
- development cooperation (6)
- diabetic nephropathy (6)
- dialogue (6)
- diffuse radiation (6)
- digitale Bildung (6)
- digitale Demokratie (6)
- domestication (6)
- dust (6)
- dust, extinction (6)
- early warning (6)
- eclogite (6)
- efficient (6)
- eicosapentaenoic acid (6)
- electrostatic interactions (6)
- elementary particles (6)
- ellipsometry (6)
- employment (6)
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- engagement (6)
- english (6)
- entropy (6)
- environmental change (6)
- enzyme (6)
- eutrophication (6)
- exoplanets (6)
- expertise (6)
- extension (6)
- eye movement (6)
- fNIRS (6)
- fabrication (6)
- facilitation (6)
- federalism (6)
- feminism (6)
- fertilization (6)
- financial markets (6)
- first passage (6)
- flooding (6)
- foreland basin (6)
- formal semantics (6)
- galaxies: abundances (6)
- galaxy evolution (6)
- gamma-ray astronomy (6)
- gamma-rays: general (6)
- gelatin (6)
- gene flow (6)
- general relativity (6)
- genes (6)
- geology (6)
- givenness (6)
- giving-up density (6)
- global (6)
- glutathione peroxidase (6)
- grammar (6)
- habituation (6)
- heart rate variability (6)
- heat (6)
- history and philosophy of astronomy (6)
- holocene (6)
- honey bee (6)
- honeybee (6)
- hybridization capture (6)
- hydrogen (6)
- hydrologische Modellierung (6)
- impacts (6)
- implicit (6)
- income (6)
- indianische Kunst (6)
- infancy (6)
- influenza (6)
- information (6)
- injury (6)
- institutions (6)
- interfaces (6)
- international relations (6)
- internet (6)
- intertextuality (6)
- introgression (6)
- invasive species (6)
- isotopes (6)
- knowledge (6)
- kosmische Strahlung (6)
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- lanthanides (6)
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- light scattering (6)
- linagliptin (6)
- liver (6)
- logic (6)
- macrophytes (6)
- magnetic nanoparticles (6)
- maximal isometric Adaptive Force (6)
- mechanism (6)
- melt inclusions (6)
- mental disorders (6)
- mental simulation (6)
- metaanalysis (6)
- metabolites (6)
- methods: analytical (6)
- micelles (6)
- microarray (6)
- microbial activity (6)
- microbial communities (6)
- mind wandering (6)
- mixed methods (6)
- molecular modeling (6)
- molecular motors (6)
- molecular rods (6)
- multicore architectures (6)
- muscle power (6)
- nanoparticle (6)
- narration (6)
- negation (6)
- network analysis (6)
- neurodegeneration (6)
- neuromuscular control (6)
- new public management (6)
- nucleation (6)
- numerical development (6)
- optimization (6)
- organic photovoltaics (6)
- organic semiconductors (6)
- p53 (6)
- palaeoclimate (6)
- parameters (6)
- pathogens (6)
- pattern formation (6)
- perpetrator (6)
- perturbation (6)
- phase transition (6)
- phase transitions (6)
- phenology (6)
- phosphate (6)
- photoelectron spectroscopy (6)
- picture naming (6)
- piezoelectricity (6)
- planning (6)
- plants (6)
- political equality (6)
- polyphenols (6)
- polystyrene (6)
- polyzwitterions (6)
- population density (6)
- porosity (6)
- postural stability (6)
- pragmatics (6)
- predictability (6)
- preferential flow (6)
- pregnancy (6)
- presidential government (6)
- presupposition (6)
- probiotics (6)
- process mining (6)
- projections (6)
- protein adsorption (6)
- protein-protein interaction (6)
- quality (6)
- quantum chemical calculations (6)
- questionnaire (6)
- radical polymerization (6)
- rainfall (6)
- rampage (6)
- randomized controlled trial (6)
- reciprocal class (6)
- reconstruction (6)
- relative index (6)
- reproduction (6)
- research projects (6)
- residual stress (6)
- respiration (6)
- restoration (6)
- retinol-binding protein 4 (6)
- return to work (6)
- risk assessment (6)
- risk factor (6)
- saccades (6)
- salinity gradient (6)
- scale development (6)
- scattering (6)
- scrambling (6)
- seismic hazard (6)
- self-concept (6)
- self-healing (6)
- sensor (6)
- sensors (6)
- silver nanoparticles (6)
- site effects (6)
- social comparison (6)
- sodium (6)
- software (6)
- software engineering (6)
- soil organic carbon (6)
- solar cells (6)
- speed (6)
- standardization (6)
- star formation (6)
- stars: kinematics and dynamics (6)
- stars: magnetic fields (6)
- stimuli-sensitive polymers (6)
- stochastic differential equations (6)
- stroke (6)
- structural equation modeling (6)
- sulfite oxidase (6)
- support vector machine (6)
- surface relief grating (6)
- surgery (6)
- synchrotron radiation (6)
- systematic literature review (6)
- tailspike protein (6)
- teachers (6)
- techniques: image processing (6)
- technology (6)
- tendinopathy (6)
- thaliana (6)
- therapist competence (6)
- therapy (6)
- thermodynamics (6)
- tomography (6)
- tourism (6)
- trade-offs (6)
- training adaptation (6)
- transcription (6)
- transcriptional memory (6)
- transcriptome (6)
- trust (6)
- typology (6)
- ultrafast dynamics (6)
- user experience (6)
- variation (6)
- ventral striatum (6)
- vesicle (6)
- visions of democracy (6)
- water management (6)
- young adults (6)
- zebrafish (6)
- öffentlicher Sektor (6)
- 19th century (5)
- 3D (5)
- 3D point clouds (5)
- AC electrokinetics (5)
- ALAN (5)
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- Atiyah-Patodi-Singer theory (5)
- Aufsatzsammlung (5)
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- Cue-based retrieval (5)
- Cultural diversity (5)
- Cyanobakterien (5)
- Cystic fibrosis (5)
- DNS (5)
- DRD4 (5)
- Datenassimilation (5)
- Datenqualität (5)
- Decision making (5)
- Decomposition (5)
- Dexamethasone (5)
- Dictyostelium discoideum (5)
- Die Vermessung der Welt (5)
- Digital Humanities (5)
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- Diversität (5)
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- Grassland (5)
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- Grundwasser (5)
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- Heiner Müller (5)
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- Holding isometric muscle action (5)
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- Pseudo-differential operators (5)
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- Public Service Motivation (5)
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- Reiseliteratur (5)
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- Residual stress (5)
- Resonant inelastic X-ray scattering (5)
- Revolution (5)
- Risk assessment (5)
- Risk reduction (5)
- Rohstoffe (5)
- Romantik (5)
- Rumänien (5)
- Russland-Tagebuch (5)
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- SEM (5)
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- STM (5)
- Sachsen (5)
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- Screening (5)
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- absorption (5)
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- anisotropy (5)
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- calculation (5)
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- collapse (5)
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- Levy walk (4)
- Lexical tone (4)
- Limnology (4)
- Linear mixed model (4)
- Lively Kernel (4)
- Livestock (4)
- Lyrik (4)
- L’Histoire des deux Indes (4)
- MATLAB (4)
- MCMC (4)
- MRI (4)
- Magnetic properties (4)
- Magnetism (4)
- Magnetotellurics (4)
- Malnutrition (4)
- Martial arts (4)
- Maschinen- und Anlagenbau (4)
- Mathematical model (4)
- Mathematikdidaktik (4)
- Maule earthquake (4)
- Max Scheler (4)
- Media (4)
- Mediterranean Sea (4)
- Mellin transform (4)
- Membrane (4)
- Metabolic syndrome (4)
- Metabolisches Syndrom (4)
- Metamorphism (4)
- Metaphysik (4)
- Methylmercury (4)
- Microcystis (4)
- Microplastics (4)
- Microsaccades (4)
- Middle childhood (4)
- Middleware (4)
- Mikropolitik (4)
- Mikrotubuli (4)
- Militärgeschichte (4)
- Mind wandering (4)
- Ministerialverwaltung (4)
- Miocene (4)
- Mixed methods (4)
- Modalität (4)
- Model-Driven Engineering (4)
- Modern Jewish history (4)
- Molecular modeling (4)
- Molybdenum (4)
- Monoclonal antibody (4)
- Monte Carlo method (4)
- Monte-Carlo simulations (4)
- Morphological processing (4)
- Motor control (4)
- Multifunctionality (4)
- Museum (4)
- NEXAFS (4)
- NGS (4)
- NZO (4)
- Nachhaltige Entwicklung (4)
- Nahostkonflikt (4)
- Nanoparticle (4)
- Nanostructure (4)
- Nanostructures (4)
- Nationalsozialismus (4)
- Natural language processing (4)
- Naturbegriff (4)
- Neotethys (4)
- Network clustering (4)
- Neue Medien (4)
- Neue Ökonomische Geographie (4)
- Neujahrsempfang (4)
- Neuroscience (4)
- New Zealand (4)
- New-Zealand (4)
- Nicaragua (4)
- Nitrate (4)
- Nonlinear Dynamics (4)
- Nordeste (4)
- Nordkorea (4)
- North Korea (4)
- Northern Asia (4)
- Nrf2 (4)
- Nuklearwaffen (4)
- Number processing (4)
- NutriAct Family Study (4)
- Oberflächengitter (4)
- Older patients (4)
- Oman (4)
- Online-Lernen (4)
- Operational momentum (4)
- Organic matter (4)
- Organisationstheorie (4)
- Orientalismus (4)
- Orinoco (4)
- Ostpolitik (4)
- Othering (4)
- Ottmar Ette (4)
- Output-Analyse (4)
- Overland flow (4)
- Ozon (4)
- PCR (4)
- PISA (4)
- PLFA (4)
- PLS (4)
- Pacific Ocean (4)
- Pacific studies (4)
- Pakistan (4)
- Palaeoclimatology (4)
- Paleogeography (4)
- Palestine (4)
- Paläoökologie (4)
- Parafoveal (4)
- Parafoveal processing (4)
- Paris (4)
- Parsing (4)
- Participation (4)
- Patagonia (4)
- Pavlovian-to-instrumental transfer (4)
- Peptide (4)
- Performanz (4)
- Perovskites (4)
- Perowskit (4)
- Persistence (4)
- Personalization (4)
- Pflanzen (4)
- Phase (4)
- Phasenübergang (4)
- Philosophische Anthropologie (4)
- Philosophy (4)
- Photoelektronenspektroskopie (4)
- Photon Density Wave spectroscopy (4)
- Phylogenetics (4)
- Placenta (4)
- Planetary rings (4)
- Plasmodium falciparum (4)
- Political Science (4)
- Politikwissenschaft (4)
- Politische Bildung (4)
- Polymerisation (4)
- Populismus (4)
- Postcolonial (4)
- Postkolonial (4)
- Postmoderne (4)
- Presse (4)
- Probabilistic Cellular Automata (4)
- Probabilistic forecasting (4)
- Process Mining (4)
- Process modeling (4)
- Production (4)
- Prognosis (4)
- Proteins (4)
- Proteom (4)
- Prototyping (4)
- Psychometric properties (4)
- Psychotherapy research (4)
- Quality management (4)
- Quantenpunkte (4)
- Quantitative Trait Locus (4)
- Quantitative Trait Locus analysis (4)
- RAFT-Polymerisation (4)
- Radiation belts (4)
- Random Forests (4)
- Rassendiskriminierung (4)
- Rauschen (4)
- Reachability (4)
- Reading development (4)
- Reading strategies (4)
- Rechtsgeschichte (4)
- Rechtspopulismus (4)
- Recovery (4)
- Recurrence plot (4)
- Reflexionskompetenz (4)
- Reformbereitschaft (4)
- Refugees (4)
- Regeneration (4)
- Regionalentwicklung (4)
- Regularisierung (4)
- Relativsätze (4)
- Religionswissenschaft (4)
- Rentenversicherung (4)
- Research School (4)
- Resistance (4)
- Resonanzenergietransfer (4)
- Respiration (4)
- Retrieval (4)
- Rezeptor (4)
- Rhizosphere (4)
- Risikofaktoren (4)
- River (4)
- Rodents (4)
- Romanische Sprachen (4)
- Röntgenspektroskopie (4)
- Rückenschmerzen (4)
- SDS PAGE (4)
- SME (4)
- SWAT (4)
- Saccharomyces cerevisiae (4)
- Salivary gland (4)
- Salts (4)
- Scale development (4)
- Schwefel (4)
- Scrum (4)
- Second World War (4)
- Secondary Education (4)
- Sediment connectivity (4)
- Sedimentologie (4)
- Sedimentology (4)
- Seismic attenuation (4)
- Selbsthilfe (4)
- Self-control (4)
- Self-employment (4)
- Self-esteem (4)
- Self-paced reading (4)
- Self-regulated learning (4)
- Self-regulation (4)
- Senescence (4)
- Sexual victimization (4)
- Shrub encroachment (4)
- Sick leave (4)
- Simulated patients (4)
- Simulationen (4)
- Sklavenhandel (4)
- Slumming (4)
- Slumtourismus (4)
- Software Engineering (4)
- Softwareentwicklung (4)
- Soil hydrology (4)
- Sol-Gel (4)
- Sorben (4)
- Sorbian identity (4)
- Sound (4)
- South Korea (4)
- Sowjetunion (4)
- Soziologie (4)
- Spanische Kolonien in Südamerika (4)
- Species distribution modelling (4)
- Speichel (4)
- Sprachverständnis (4)
- St. Petersburg (4)
- Staat (4)
- Staatsbibliothek zu Berlin (4)
- Stabilität (4)
- Stadtentwicklung (4)
- Standardized patients (4)
- Staphylococcus aureus (4)
- Start-Up Subsidies (4)
- Start-up subsidies (4)
- Statistical methods (4)
- Statistik öffentlicher Unternehmen (4)
- Sterne (4)
- Sternentstehung (4)
- Strategic foresight (4)
- Structural geology (4)
- Structure elucidation (4)
- Studie (4)
- Studienabbruch (4)
- Städte (4)
- Subduktionszone (4)
- Subjektorientierung (4)
- Sun (4)
- Surface roughness (4)
- Survival (4)
- Swedish (4)
- Synchrotronstrahlung (4)
- System (4)
- Systematische Musikpädagogik (4)
- Systems biology (4)
- Süd- und Mittelamerika (4)
- TPACK (4)
- TSNMRS (4)
- Tailspike (4)
- Teachers (4)
- Team sports (4)
- Teamwork (4)
- Technik (4)
- Telemedicine (4)
- Tenside (4)
- Therapie (4)
- Thermal effects (4)
- Thermochronology (4)
- Thermoresponsive (4)
- Ti-6Al-4V (4)
- ToF-SIMS (4)
- Trace elements (4)
- Transdisziplinarität (4)
- Transfer function (4)
- Transkriptom (4)
- Transzendenz (4)
- Treibhausgase (4)
- Triassic (4)
- Tropical forest (4)
- Tso Moriri Lake (4)
- Turkish-German bilingualism (4)
- Typ-2-Diabetes (4)
- Typologie (4)
- Tyramin (4)
- UAV (4)
- UV (4)
- Umweltpolitik (4)
- Uncertainties (4)
- Underspecification (4)
- Understanding (4)
- Unternehmen (4)
- V-ATPase (4)
- VIIRS DNB (4)
- Van Allen Probes (4)
- Verfassungsrecht (4)
- Verhandlungen (4)
- Verification (4)
- Vernetzung (4)
- Verteidigungspolitik (4)
- Verwaltungswissenschaft (4)
- Virtualisierung (4)
- Virtuelle Maschinen (4)
- Vulkan (4)
- WA-PLS (4)
- Wasserqualität (4)
- Weathering (4)
- Weight (4)
- Weight bias internalization (4)
- Wellenleiter (4)
- West Antarctica (4)
- Wetter (4)
- Widerstand (4)
- Wirtschaftspolitik (4)
- Wissen (4)
- Wohlbefinden (4)
- Work ability (4)
- X-ray absorption (4)
- X-ray emission spectroscopy (4)
- X-ray photoelectron spectroscopy (4)
- X-ray scattering (4)
- Xanthomonas (4)
- Zaremba problem (4)
- academic self-concept (4)
- accretion, accretion disks (4)
- achilles tendinopathy (4)
- actin (4)
- action language (4)
- action processing (4)
- action words (4)
- actuation (4)
- adaptive force (4)
- additive particles (4)
- adherence (4)
- adipogenesis (4)
- adjectives (4)
- advanced dynamic flow (4)
- aerosol (4)
- aerosol size distribution (4)
- affective (4)
- agency (4)
- agent-based model (4)
- agility (4)
- aldehyde oxidase (4)
- allelopathy (4)
- alternatives (4)
- anaphors (4)
- anorexia nervosa (4)
- antifouling (4)
- antilocality (4)
- aphids (4)
- aquatic ecosystems (4)
- aqueous-solution (4)
- arabidopsis (4)
- arabidopsis-thaliana (4)
- argumentation (4)
- arsenolipids present (4)
- association (4)
- associations (4)
- astronomy (4)
- attachment (4)
- attenuation tomography (4)
- authentication (4)
- authenticity (4)
- authority (4)
- automatic evaluation (4)
- automatic evaluations (4)
- autophagy (4)
- balance training (4)
- bibliometrics (4)
- bildende Kunst (4)
- biodiversity exploratories (4)
- bioelectrocatalysis (4)
- biogenic amine (4)
- biogeochemistry (4)
- biological age (4)
- biological invasions (4)
- biomarkers (4)
- bis-MGD (4)
- black hole physics (4)
- body waves (4)
- brushes (4)
- brushite (4)
- buildings (4)
- bulimia nervosa (4)
- bulk heterojunction (4)
- business (4)
- calcium carbonate (4)
- capacity building (4)
- capital market (4)
- carbon isotopes (4)
- carbon nitrides (4)
- catchments (4)
- categories (4)
- cell cycle (4)
- cell migration (4)
- cell wall (4)
- cellular signalling (4)
- change management (4)
- characteristics (4)
- chitosan (4)
- chronic pain (4)
- circular dichroism (4)
- civil society (4)
- civil war (4)
- cleft (4)
- climate adaptation (4)
- climate change impacts (4)
- climate extremes (4)
- clinical sample (4)
- clone (4)
- co-occurrence analysis (4)
- coarticulation (4)
- coating (4)
- cod-liver (4)
- coefficient (4)
- coercion (4)
- coevolution (4)
- cognitive enhancement (4)
- cognitive modeling (4)
- collaborative consumption (4)
- collagen (4)
- colonization (4)
- combat sports (4)
- common vole (4)
- community structure (4)
- competencies (4)
- compliance (4)
- computer science (4)
- computer science education (4)
- computer-based training (4)
- conceptualization (4)
- concrete (4)
- conduct problems (4)
- conductivity (4)
- confocal microscopy (4)
- consequences (4)
- constructivism (4)
- content areas (4)
- content knowledge (4)
- content-addressable memory (4)
- conversion (4)
- copper(II) (4)
- core (4)
- coreference (4)
- corpus linguistics (4)
- cosmology (4)
- coupling (4)
- covariance (4)
- critical theory (4)
- crop (4)
- crop production (4)
- cross-cultural comparison (4)
- cross-curriculum (4)
- crosslinking (4)
- crust (4)
- cultural identity (4)
- cyberbullying victimization (4)
- cylindrospermopsin (4)
- cystic fibrosis (4)
- cytochrome c (4)
- data mining (4)
- data preparation (4)
- data quality (4)
- decarbonization (4)
- deformation (4)
- degenerative disc disease (4)
- democratic theory (4)
- demography (4)
- deutsch-jüdische Geschichte (4)
- developing countries (4)
- diachrony (4)
- diagnostic competence (4)
- diagnostics (4)
- dictyostelium (4)
- differential operators (4)
- digitization (4)
- disability (4)
- discourse production (4)
- distributed systems (4)
- diversification (4)
- dog (4)
- dropout (4)
- drought stress (4)
- droughts (4)
- drylands (4)
- dual-process (4)
- dyadic coping (4)
- dye (4)
- dye removal (4)
- dynamic capabilities (4)
- dynamical models (4)
- dynamical systems (4)
- e-government (4)
- eating disorders (4)
- eco-evolutionary experience (4)
- economics (4)
- edge effect (4)
- electron microscopy (4)
- electron paramagnetic resonance (4)
- electron-transfer (4)
- electrostatics (4)
- elliptic operator (4)
- emissions (4)
- emotional expression (4)
- emotional intelligence (4)
- employment policy (4)
- emulsion polymerization (4)
- endurance exercise (4)
- enhancement (4)
- entanglement (4)
- environmental economics (4)
- environmental filtering (4)
- environmental policy (4)
- equality (4)
- errata, addenda (4)
- ethnic differences (4)
- ethnic identity (4)
- evaluative study (4)
- evaporites (4)
- event-related brain potentials (4)
- everyday life (4)
- evolutionary history (4)
- exchange (4)
- exercise intensity (4)
- expansion microscopy (4)
- exploratory-behavior (4)
- extracellular matrix (4)
- eye-movement monitoring (4)
- face perception (4)
- fatty acid metabolism (4)
- fatty-acids (4)
- fault (4)
- faults (4)
- feature selection (4)
- fence ecology (4)
- ferroelectrets (4)
- ferroelectric polymers (4)
- ferroelectricity (4)
- financial crisis (4)
- financial institutions (4)
- finger counting (4)
- fire (4)
- firm performance (4)
- first language acquisition (4)
- first-passage (4)
- fiscal federalism (4)
- fixation durations (4)
- flavonoid (4)
- floral scent (4)
- flower development (4)
- fluctuating light (4)
- fluid inclusions (4)
- focal adhesion (4)
- food choice (4)
- food neophilia (4)
- formal verification (4)
- forschendes Lernen (4)
- fractional dynamics (4)
- frequency analysis (4)
- fruit (4)
- frustration (4)
- functional response (4)
- fungi (4)
- galaxies: star formation (4)
- garden-path effect (4)
- gaze-contingent displays (4)
- gene regulatory networks (4)
- genealogy (4)
- genetic diversity (4)
- genome-wide association (4)
- geomorphometry (4)
- geothermal (4)
- geothermal energy (4)
- global jets (4)
- global positioning system (4)
- global sensitivity analysis (4)
- glomerular filtration rate (4)
- glucosinolates (4)
- glutathione (4)
- glycopolymers (4)
- good governance (4)
- government (4)
- graph theory (4)
- graph transformation (4)
- graph transformation systems (4)
- graphs (4)
- ground reaction force (4)
- groundwater recharge (4)
- group field theory (4)
- habitat (4)
- handgrip strength (4)
- handwriting (4)
- hazard (4)
- health care (4)
- healthcare (4)
- heat stress (4)
- helical magnetic fields (4)
- hemocompatibility (4)
- heritage language (4)
- high-intensity interval training (4)
- holding capacity (4)
- holding isometric muscle action (HIMA) (4)
- homeostasis (4)
- hormones (4)
- hybrid (4)
- hybrid perovskites (4)
- hydrological modeling (4)
- hydrological models (4)
- hypoxia (4)
- ill-posed problem (4)
- imitation (4)
- immune system (4)
- immunology (4)
- implementation (4)
- implicit attitude (4)
- in vitro (4)
- in-service teacher training (4)
- industry 4.0 (4)
- inertial measurement unit (4)
- inflammatory bowel disease (4)
- informatics (4)
- infrastructure (4)
- inorganic perovskites (4)
- institutional reform (4)
- insulin signaling (4)
- interactional linguistics (4)
- interception (4)
- intergovernmental relations (4)
- international bureaucracies (4)
- international trade (4)
- internationale Beziehungen (4)
- internationaler Handel (4)
- intimate partner violence (4)
- intrusion detection (4)
- ionic conductivity (4)
- ionic strength (4)
- joint Simon effect (4)
- joint action (4)
- justice (4)
- karst (4)
- kidney (4)
- kidney transplantation (4)
- knickpoint (4)
- knowledge transfer (4)
- komplexe Systeme (4)
- körperliche Aktivität (4)
- künstliche Intelligenz (4)
- land-use intensity (4)
- laser pulses (4)
- learning environment (4)
- left periphery (4)
- lexical access (4)
- lexicon (4)
- lichens (4)
- life satisfaction (4)
- lifestyle (4)
- lifetime income (4)
- light variability (4)
- line: identification (4)
- linear hypersubstitution (4)
- linear mixed models (4)
- lipidomics (4)
- literacy acquisition (4)
- loading rate (4)
- locomotion (4)
- locus coeruleus (4)
- long-distance dispersal (4)
- low-back-pain (4)
- lower critical solution temperature (4)
- lutein (4)
- lutherische Theologie (4)
- machine (4)
- macrocycles (4)
- magnetostriction (4)
- magnetotellurics (4)
- mammals (4)
- manifold with singularities (4)
- masculinity (4)
- mathematics education (4)
- mathematics instruction (4)
- meadow (4)
- measurement invariance (4)
- mechanical tendinous oscillations (4)
- media use (4)
- memory effects (4)
- mesenchymal stem cells (4)
- mesoporous materials (4)
- metabolic engineering (4)
- metabolic regulation (4)
- metabolite (4)
- metacognition (4)
- metadata (4)
- meteorological drought (4)
- methanogenic archaea (4)
- methyl orange (4)
- methylene blue (4)
- methylmercury (4)
- miRNAs (4)
- micro-CT (4)
- microRNA (4)
- microlensing (4)
- micronutrients (4)
- mid-temperature transition (4)
- mild cognitive impairment (4)
- mitochondrial DNA (4)
- mitosis (4)
- mixtures (4)
- mobile links (4)
- model-driven engineering (4)
- modernization (4)
- molecular evolution (4)
- molecules (4)
- monoclonal antibodies (4)
- mood (4)
- morphogenesis (4)
- motivational interviewing (4)
- mouse (4)
- mowing (4)
- multilevel analysis (4)
- muscle oxygen saturation (4)
- museum specimens (4)
- music information retrieval (4)
- musikpädagogische Forschung (4)
- n-alkanes (4)
- nachhaltige Chemie (4)
- nanolenses (4)
- narrative (4)
- natural hazard (4)
- naturalism (4)
- nature conservation (4)
- neoliberalism (4)
- nested graph conditions (4)
- network reconstruction (4)
- neurodegenerative diseases (4)
- neuroenhancement (4)
- neuronal networks (4)
- neurotoxicity (4)
- neutrinos (4)
- new species (4)
- next generation sequencing (NGS) (4)
- nominalization (4)
- non-alcoholic fatty liver disease (4)
- non-linear dynamics (4)
- non-native speakers (4)
- non-photorealistic rendering (4)
- nonlinear operator (4)
- noun (4)
- numerical (4)
- numerical model (4)
- ocean (4)
- ocean acidification (4)
- ocean color remote sensing (4)
- ontogeny (4)
- operating systems (4)
- optimal foraging (4)
- optimal transport (4)
- organ size (4)
- organic carbon (4)
- organische Chemie (4)
- organische Halbleiter (4)
- osmotic-pressure (4)
- osteoblast (4)
- other-race effect (4)
- paleoaltimetry (4)
- paleoclimatology (4)
- paleogenomics (4)
- paleolimnology (4)
- parafoveal-on-foveal effect (4)
- part 1 (4)
- partial clone (4)
- partial least squares (4)
- particle acceleration (4)
- parties (4)
- pathway (4)
- patient education (4)
- people (4)
- performance information (4)
- performance management (4)
- performance measurement (4)
- permafrost thaw (4)
- persulfide (4)
- phase (4)
- phase dynamics (4)
- phase oscillators (4)
- phenols (4)
- phenotyping (4)
- phonology (4)
- phosphatidylserine (4)
- photofragmentation (4)
- photogrammetry (4)
- photoisomerization (4)
- photon density wave spectroscopy (4)
- photooxygenation (4)
- photophysics (4)
- phototaxis (4)
- photovoltaic devices (4)
- physiological consequences (4)
- picture-word interference (4)
- planetary rings (4)
- planets and satellites: atmospheres (4)
- plant community (4)
- plant development (4)
- plant growth (4)
- plant population and community dynamics (4)
- plasma membrane (4)
- plasma-membrane (4)
- platelets (4)
- playa (4)
- plume (4)
- pneumonia (4)
- point process (4)
- point-of-care (4)
- policy advice (4)
- policy analysis (4)
- political elites (4)
- politische Eliten (4)
- poly(ethylene glycol) (4)
- polyadenylation (4)
- polyelectrolyte adsorption (4)
- polysemy (4)
- polysulfobetaine (4)
- population structure (4)
- porous carbon (4)
- poröse Materialien (4)
- potato (4)
- pragmaticalisation (4)
- pre-school (4)
- preactivation (4)
- precuneus (4)
- precursor (4)
- preference assessment (4)
- primary care (4)
- principal component analysis (4)
- privatization (4)
- proactive aggression (4)
- proactivity (4)
- probabilistic timed systems (4)
- problem-solving (4)
- process analytical technology (4)
- prosocial behavior (4)
- protein engineering (4)
- proteinuria (4)
- proteome (4)
- psychosocial moderators (4)
- psychotherapy process (4)
- pubertal timing (4)
- public policy (4)
- qualitative Analyse (4)
- qualitative content analysis (4)
- qualitative research (4)
- quantum gravity (4)
- quorum sensing (4)
- racism (4)
- radicals (4)
- random walk (4)
- random walks (4)
- ranking (4)
- rape myth acceptance (4)
- rat (4)
- rate of perceived exertion (4)
- reactive aggression (4)
- reading motivation (4)
- receptor (4)
- recollection (4)
- recombinant inbred line (4)
- recurrence (4)
- recurrence plot (4)
- redox (4)
- redox state (4)
- regime shift (4)
- regression (4)
- regularity (4)
- relatedness (4)
- religiosity (4)
- remagnetization (4)
- reproductive strategies (4)
- requirements (4)
- research (4)
- research data management (4)
- research design (4)
- research school (4)
- resistin (4)
- resocialization (4)
- revolution (4)
- rifting (4)
- risk analysis (4)
- risk perceptions (4)
- risk reduction (4)
- risk-factors (4)
- river incision (4)
- rolling averages (4)
- root traits (4)
- rotifer (4)
- run time analysis (4)
- runners (4)
- saliva (4)
- salivary alpha-amylase (4)
- salt pan (4)
- savanna (4)
- scaffolding (4)
- scenario (4)
- scenario analysis (4)
- school adjustment (4)
- school children (4)
- school mathematics (4)
- sea level (4)
- secondary school (4)
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- coherency (3)
- cohomology (3)
- coiled coil (3)
- cold (3)
- collective dynamics (3)
- collective guilt (3)
- collective team identification (3)
- collusion (3)
- colon cancer (3)
- color (3)
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- combat sport (3)
- comets: individual: 67P/Churyumov-Gerasimenko (3)
- community assembly (3)
- comorbidities (3)
- companies (3)
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- compensatory dynamics (3)
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- complementarity (3)
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- conformation (3)
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- constraint (3)
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- consumption (3)
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- contact (3)
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- contestation (3)
- continental lithosphere (3)
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- contrastive focus (3)
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- convolutional neural network (3)
- coping styles (3)
- copolymerization (3)
- coronal mass ejections (CMEs) (3)
- coronary bypass grafting (3)
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- corpus analysis (3)
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- cost-benefit analysis (3)
- counting process (3)
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- creep (3)
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- decision tree (3)
- decompositon (3)
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- deep convection (3)
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- deep-sea (3)
- defense against predation (3)
- definition (3)
- deforestation (3)
- degraded DNA (3)
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- democratisation (3)
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- denitrification (3)
- density functional theory (3)
- denudation (3)
- deposition (3)
- depressive disorder (3)
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- diagnosis (3)
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- dielectric relaxation spectroscopy (3)
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- differential gene expression (3)
- diffraction (3)
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- digital elevation model (3)
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- establishment (3)
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- faking (3)
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- framework (3)
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- fricative (3)
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- functional (3)
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- galaxies: fundamental parameters (3)
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- gels (3)
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- gene duplication (3)
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- general (3)
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- kana (3)
- kanji (3)
- keratinocytes (3)
- kernel density estimation (3)
- kernel methods (3)
- kettle hole (3)
- kinetic instabilities (3)
- knowledge sharing (3)
- kognitive Modellierung (3)
- kollektives Gedächtnis (3)
- komplexes Netzwerk (3)
- körperliche Fitness (3)
- l-cysteine desulfurase (3)
- labor supply (3)
- lactate (3)
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- langevin equation (3)
- language attitudes (3)
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- large-scale (3)
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- last glacial period (3)
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- latitude (3)
- latitudinal gradient (3)
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- leaf (3)
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- logarithmic source condition (3)
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- modularity (3)
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- natural habitats (3)
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- need satisfaction (3)
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- neural (3)
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- protonation (3)
- prototype-willingness-model (3)
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- pseudo-differential boundary value problems (3)
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- rationalism (3)
- reactance (3)
- reaction mechanisms (3)
- reading development (3)
- reading fluency (3)
- real-time (3)
- rearrangement (3)
- reception (3)
- reciprocal processes (3)
- recommendations (3)
- record (3)
- record linkage (3)
- recurrence quantification analysis (3)
- red meat (3)
- redox regulation (3)
- reductase (3)
- reference material (3)
- referential choice (3)
- refinement (3)
- reforms (3)
- refugee (3)
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- regime shifts (3)
- regionalisation (3)
- regioselectivity (3)
- regression tree (3)
- reionization (3)
- relationship constellations (3)
- relationships (3)
- relative clause (3)
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- relevance (3)
- remission (3)
- remote sensing data (3)
- remote teaching (3)
- replacement (3)
- replication (3)
- representation learning (3)
- reproducing kernel Hilbert space (3)
- residue (3)
- resocialisation (3)
- resolution (3)
- resonance energy-transfer (3)
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- resurrection plants (3)
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- reward (3)
- rhetoric (3)
- rhizosphere (3)
- right-wing populism (3)
- ring current electrons (3)
- risk attitudes (3)
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- river (3)
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- robustness (3)
- rodent (3)
- role-play (3)
- rule of law (3)
- rules (3)
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- rural areas (3)
- sACC (3)
- salinity (3)
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- salt (3)
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- sample preparation (3)
- satellite data (3)
- scalability (3)
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- school development (3)
- schwarze Löcher (3)
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- secondary metabolites (3)
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- semantic (3)
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- separation (3)
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- shape-memory polymer (3)
- short-read mapping (3)
- sibilant (3)
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- signalling (3)
- similarity (3)
- simulation model (3)
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- starch synthase (3)
- stars: (3)
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- study (3)
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- syllables (3)
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- task (3)
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- test (3)
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- theorem (3)
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- (Anti)aromaticity (2)
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- ) (2)
- 1 (2)
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- 100. Geburtstag (2)
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- 1D (2)
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- 2-Azaspiro[4.5]deca-1-ones (2)
- 2-deoxy-D-ribose-5-phoshphate aldolase (2)
- 2008 Wenchuan earthquake (2)
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- 47A52 (2)
- 5-day waves (2)
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- 65R20 (2)
- 65R32 (2)
- 78A46 (2)
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Due to the high concentration of people and infrastructures in European cities, the possible impacts of climate change are particularly high (cities' social, economic and technical vulnerabilities). Adaptation measures to reduce the sensitivity of a city to climate risks are therefore of particular importance. Nevertheless, it is also common to develop compact and dense urban areas to reduce urban sprawl. Urban infill development and sustainable spatial climate policies are thus in apparent conflict with each other. This article examines how German cities deal with the tensions between these two policy fields. Using six case studies, a new heuristic analysis method is applied. This study identifies three key governance aspects that are essential for promoting the joint implementation: instruments, organisation and interaction. Based on our case studies, we conclude that successful implementation can only be achieved through integrative governance including all three domains.
Land degradation and water availability in semi-arid regions are interdependent challenges for management that are influenced by climatic and anthropogenic changes. Erosion and high sediment loads in rivers cause reservoir siltation and decrease storage capacity, which pose risk on water security for citizens, agriculture, and industry. In regions where resources for management are limited, identifying spatial-temporal variability of sediment sources is crucial to decrease siltation. Despite widespread availability of rigorous methods, approaches simplifying spatial and temporal variability of erosion are often inappropriately applied to very data sparse semi-arid regions. In this work, we review existing approaches for mapping erosional hotspots, and provide an example of spatial-temporal mapping approach in two case study regions. The barriers limiting data availability and their effects on erosion mapping methods, their validation, and resulting prioritization of leverage management areas are discussed.
Land degradation and water availability in semi-arid regions are interdependent challenges for management that are influenced by climatic and anthropogenic changes. Erosion and high sediment loads in rivers cause reservoir siltation and decrease storage capacity, which pose risk on water security for citizens, agriculture, and industry. In regions where resources for management are limited, identifying spatial-temporal variability of sediment sources is crucial to decrease siltation. Despite widespread availability of rigorous methods, approaches simplifying spatial and temporal variability of erosion are often inappropriately applied to very data sparse semi-arid regions. In this work, we review existing approaches for mapping erosional hotspots, and provide an example of spatial-temporal mapping approach in two case study regions. The barriers limiting data availability and their effects on erosion mapping methods, their validation, and resulting prioritization of leverage management areas are discussed.
Actin is one of the most highly conserved proteins in eukaryotes and distinct actin-related proteins with filament-forming properties are even found in prokaryotes. Due to these commonalities, actin-modulating proteins of many species share similar structural properties and proposed functions. The polymerization and depolymerization of actin are critical processes for a cell as they can contribute to shape changes to adapt to its environment and to move and distribute nutrients and cellular components within the cell. However, to what extent functions of actin-binding proteins are conserved between distantly related species, has only been addressed in a few cases. In this work, functions of Coronin-A (CorA) and Actin-interacting protein 1 (Aip1), two proteins involved in actin dynamics, were characterized. In addition, the interchangeability and function of Aip1 were investigated in two phylogenetically distant model organisms. The flowering plant Arabidopsis thaliana (encoding two homologs, AIP1-1 and AIP1-2) and in the amoeba Dictyostelium discoideum (encoding one homolog, DdAip1) were chosen because the functions of their actin cytoskeletons may differ in many aspects. Functional analyses between species were conducted for AIP1 homologs as flowering plants do not harbor a CorA gene.
In the first part of the study, the effect of four different mutation methods on the function of Coronin-A protein and the resulting phenotype in D. discoideum was revealed in two genetic knockouts, one RNAi knockdown and a sudden loss-of-function mutant created by chemical-induced dislocation (CID). The advantages and disadvantages of the different mutation methods on the motility, appearance and development of the amoebae were investigated, and the results showed that not all observed properties were affected with the same intensity. Remarkably, a new combination of Selection-Linked Integration and CID could be established.
In the second and third parts of the thesis, the exchange of Aip1 between plant and amoeba was carried out. For A. thaliana, the two homologs (AIP1-1 and AIP1-2) were analyzed for functionality as well as in D. discoideum. In the Aip1-deficient amoeba, rescue with AIP1-1 was more effective than with AIP1-2. The main results in the plant showed that in the aip1-2 mutant background, reintroduced AIP1-2 displayed the most efficient rescue and A. thaliana AIP1-1 rescued better than DdAip1. The choice of the tagging site was important for the function of Aip1 as steric hindrance is a problem. The DdAip1 was less effective when tagged at the C-terminus, while the plant AIP1s showed mixed results depending on the tag position. In conclusion, the foreign proteins partially rescued phenotypes of mutant plants and mutant amoebae, despite the organisms only being very distantly related in evolutionary terms.
Ammonium salts are a reservoir of nitrogen on a cometary nucleus and possibly on some asteroids
(2020)
The measured nitrogen-to-carbon ratio in comets is lower than for the Sun, a discrepancy which could be alleviated if there is an unknown reservoir of nitrogen in comets. The nucleus of comet 67P/Churyumov-Gerasimenko exhibits an unidentified broad spectral reflectance feature around 3.2 micrometers, which is ubiquitous across its surface. On the basis of laboratory experiments, we attribute this absorption band to ammonium salts mixed with dust on the surface. The depth of the band indicates that semivolatile ammonium salts are a substantial reservoir of nitrogen in the comet, potentially dominating over refractory organic matter and more volatile species. Similar absorption features appear in the spectra of some asteroids, implying a compositional link between asteroids, comets, and the parent interstellar cloud.
Ammonium salts are a reservoir of nitrogen on a cometary nucleus and possibly on some asteroids
(2020)
The measured nitrogen-to-carbon ratio in comets is lower than for the Sun, a discrepancy which could be alleviated if there is an unknown reservoir of nitrogen in comets. The nucleus of comet 67P/Churyumov-Gerasimenko exhibits an unidentified broad spectral reflectance feature around 3.2 micrometers, which is ubiquitous across its surface. On the basis of laboratory experiments, we attribute this absorption band to ammonium salts mixed with dust on the surface. The depth of the band indicates that semivolatile ammonium salts are a substantial reservoir of nitrogen in the comet, potentially dominating over refractory organic matter and more volatile species. Similar absorption features appear in the spectra of some asteroids, implying a compositional link between asteroids, comets, and the parent interstellar cloud.
The shape of a defense-growth trade-off governs seasonal trait dynamics in natural phytoplankton
(2020)
Theory predicts that trade-offs, quantifying costs of functional trait adjustments, crucially affect community trait adaptation to altered environmental conditions, but empirical verification is scarce. We evaluated trait dynamics (antipredator defense, maximum growth rate, and phosphate affinity) of a lake phytoplankton community in a seasonally changing environment, using literature trait data and 21 years of species-resolved high-frequency biomass measurements. The trait data indicated a concave defense-growth trade-off, promoting fast-growing species with intermediate defense. With seasonally increasing grazing pressure, the community shifted toward higher defense levels at the cost of lower growth rates along the trade-off curve, while phosphate affinity explained some deviations from it. We discuss how low fitness differences of species, inferred from model simulations, in concert with stabilizing mechanisms, e.g., arising from further trait dimensions, may lead to the observed phytoplankton diversity. In conclusion, quantifying trade-offs is key for predictions of community trait adaptation and biodiversity under environmental change.
A method for the fabrication of well-defined metallic nanostructures is presented here in a simple and straightforward fashion. As an alternative to lithographic techniques, this routine employs microcontact printing utilizing wrinkled stamps, which are prepared from polydimethylsiloxane (PDMS), and includes the formation of hydrophobic stripe patterns on a substrate via the transfer of oligomeric PDMS. Subsequent backfilling of the interspaces between these stripes with a hydroxyl-functional poly(2-vinyl pyridine) then provides the basic pattern for the deposition of citrate-stabilized gold nanoparticles promoted by electrostatic interaction. The resulting metallic nanostripes can be further customized by peeling off particles in a second microcontact printing step, which employs poly(ethylene imine) surface-decorated wrinkled stamps, to form nanolattices. Due to the independent adjustability of the period dimensions of the wrinkled stamps and stamp orientation with respect to the substrate, particle arrays on the (sub)micro-scale with various kinds of geometries are accessible in a straightforward fashion. This work provides an alternative, cost-effective, and scalable surface-patterning technique to fabricate nanolattice structures applicable to multiple types of functional nanoparticles. Being a top-down method, this process could be readily implemented into, e.g., the fabrication of optical and sensing devices on a large scale.
Understanding the hydrologic connectivity between kettle holes and shallow groundwater, particularly in reaction to the highly variable local meteorological conditions, is of paramount importance for tracing water in a hydro(geo)logically complex landscape and thus for integrated water resource management. This article is aimed at identifying the dominant hydrological processes affecting the kettle holes' water balance and their interactions with the shallow groundwater domain in the Uckermark region, located in the north-east of Germany. For this reason, based on the stable isotopes of oxygen (delta O-18) and hydrogen (delta H-2), an isotopic mass balance model was employed to compute the evaporative loss of water from the kettle holes from February to August 2017. Results demonstrated that shallow groundwater inflow may play the pivotal role in the processes taking part in the hydrology of the kettle holes in the Uckermark region. Based on the calculated evaporation/inflow (E/I) ratios, most of the kettle holes (86.7%) were ascertained to have a partially open, flow-through-dominated system. Moreover, we identified an inverse correlation between E/I ratios and the altitudes of the kettle holes. The same holds for electrical conductivity (EC) and the altitudes of the kettle holes. In accordance with the findings obtained from this study, a conceptual model explaining the interaction between the shallow groundwater and the kettle holes of Uckermark was developed. The model exhibited that across the highest altitudes, the recharge kettle holes are dominant, where a lower ratio of E/I and a lower EC was detected. By contrast, the lowest topographical depressions represent the discharge kettle holes, where a higher ratio of E/I and EC could be identified. The kettle holes existing in between were categorized as flow-through kettle holes through which the recharge takes place from one side and discharge from the other side.
Understanding the hydrologic connectivity between kettle holes and shallow groundwater, particularly in reaction to the highly variable local meteorological conditions, is of paramount importance for tracing water in a hydro(geo)logically complex landscape and thus for integrated water resource management. This article is aimed at identifying the dominant hydrological processes affecting the kettle holes' water balance and their interactions with the shallow groundwater domain in the Uckermark region, located in the north-east of Germany. For this reason, based on the stable isotopes of oxygen (delta O-18) and hydrogen (delta H-2), an isotopic mass balance model was employed to compute the evaporative loss of water from the kettle holes from February to August 2017. Results demonstrated that shallow groundwater inflow may play the pivotal role in the processes taking part in the hydrology of the kettle holes in the Uckermark region. Based on the calculated evaporation/inflow (E/I) ratios, most of the kettle holes (86.7%) were ascertained to have a partially open, flow-through-dominated system. Moreover, we identified an inverse correlation between E/I ratios and the altitudes of the kettle holes. The same holds for electrical conductivity (EC) and the altitudes of the kettle holes. In accordance with the findings obtained from this study, a conceptual model explaining the interaction between the shallow groundwater and the kettle holes of Uckermark was developed. The model exhibited that across the highest altitudes, the recharge kettle holes are dominant, where a lower ratio of E/I and a lower EC was detected. By contrast, the lowest topographical depressions represent the discharge kettle holes, where a higher ratio of E/I and EC could be identified. The kettle holes existing in between were categorized as flow-through kettle holes through which the recharge takes place from one side and discharge from the other side.
In this article, we propose an all-in-one statement which includes existence, uniqueness, regularity, and numerical approximations of mild solutions for a class of stochastic partial differential equations (SPDEs) with non-globally monotone nonlinearities. The proof of this result exploits the properties of an existing fully explicit space-time discrete approximation scheme, in particular the fact that it satisfies suitable a priori estimates. We also obtain almost sure and strong convergence of the approximation scheme to the mild solutions of the considered SPDEs. We conclude by applying the main result of the article to the stochastic Burgers equations with additive space-time white noise.
An exceptionally strong stationary planetary wave with Zonal Wavenumber 1 led to a sudden stratospheric warming (SSW) in the Southern Hemisphere in September 2019. Ionospheric data from European Space Agency's Swarm satellite constellation mission show prominent 6-day variations in the dayside low-latitude region at this time, which can be attributed to forcing from the middle atmosphere by the Rossby normal mode "quasi-6-day wave" (Q6DW). Geopotential height measurements by the Microwave Limb Sounder aboard National Aeronautics and Space Administration's Aura satellite reveal a burst of global Q6DW activity in the mesosphere and lower thermosphere during the SSW, which is one of the strongest in the record. The Q6DW is apparently generated in the polar stratosphere at 30-40 km, where the atmosphere is unstable due to strong vertical wind shear connected with planetary wave breaking. These results suggest that an Antarctic SSW can lead to ionospheric variability through wave forcing from the middle atmosphere.
Plain Language Summary: A sudden stratospheric warming (SSW) is an extreme wintertime polar meteorological phenomenon occurring mostly over the Arctic region. Studies have shown that Arctic SSW can influence the entire atmosphere. In September 2019, a rare SSW event occurred in the Antarctic region, providing an opportunity to investigate its broader impact on the whole atmosphere. We present observations from the middle atmosphere and ionosphere during this event, noting unusually strong wave activity throughout this region. Our results suggest that an Antarctic SSW can have a significant impact on the whole atmosphere system similar to those due to Arctic events.
An exceptionally strong stationary planetary wave with Zonal Wavenumber 1 led to a sudden stratospheric warming (SSW) in the Southern Hemisphere in September 2019. Ionospheric data from European Space Agency's Swarm satellite constellation mission show prominent 6-day variations in the dayside low-latitude region at this time, which can be attributed to forcing from the middle atmosphere by the Rossby normal mode "quasi-6-day wave" (Q6DW). Geopotential height measurements by the Microwave Limb Sounder aboard National Aeronautics and Space Administration's Aura satellite reveal a burst of global Q6DW activity in the mesosphere and lower thermosphere during the SSW, which is one of the strongest in the record. The Q6DW is apparently generated in the polar stratosphere at 30-40 km, where the atmosphere is unstable due to strong vertical wind shear connected with planetary wave breaking. These results suggest that an Antarctic SSW can lead to ionospheric variability through wave forcing from the middle atmosphere.
Plain Language Summary: A sudden stratospheric warming (SSW) is an extreme wintertime polar meteorological phenomenon occurring mostly over the Arctic region. Studies have shown that Arctic SSW can influence the entire atmosphere. In September 2019, a rare SSW event occurred in the Antarctic region, providing an opportunity to investigate its broader impact on the whole atmosphere. We present observations from the middle atmosphere and ionosphere during this event, noting unusually strong wave activity throughout this region. Our results suggest that an Antarctic SSW can have a significant impact on the whole atmosphere system similar to those due to Arctic events.
Studies on the diversity, distribution and ecological role of Saprolegniales (Oomycota) in freshwater ecosystems are currently receiving attention due to a greater understanding of their role in carbon cycling in various aquatic ecosystems. In this study, we characterized several Saprolegniales species isolated from Anzali lagoon, Gilan province, Iran, using morphological and molecular methods. Four species of Saprolegnia were identified, including S. anisospora and S. diclina as first reports for Iran, as well as Achlya strains, which were closely related to A. bisexualis, A. debaryana and A. intricata. Evaluation of the ligno-, cellulo- and chitinolytic activities was performed using plate assay methods. Most of the Saprolegniales isolates were obtained in autumn, and nearly 50% of the strains showed chitinolytic and cellulolytic activities. However, only a few Saprolegniales strains showed lignolytic activities. This study has important implications for better understanding the ecological niche of oomycetes, and to differentiate them from morphologically similar, but functionally different aquatic fungi in freshwater ecosystems.
Studies on the diversity, distribution and ecological role of Saprolegniales (Oomycota) in freshwater ecosystems are currently receiving attention due to a greater understanding of their role in carbon cycling in various aquatic ecosystems. In this study, we characterized several Saprolegniales species isolated from Anzali lagoon, Gilan province, Iran, using morphological and molecular methods. Four species of Saprolegnia were identified, including S. anisospora and S. diclina as first reports for Iran, as well as Achlya strains, which were closely related to A. bisexualis, A. debaryana and A. intricata. Evaluation of the ligno-, cellulo- and chitinolytic activities was performed using plate assay methods. Most of the Saprolegniales isolates were obtained in autumn, and nearly 50% of the strains showed chitinolytic and cellulolytic activities. However, only a few Saprolegniales strains showed lignolytic activities. This study has important implications for better understanding the ecological niche of oomycetes, and to differentiate them from morphologically similar, but functionally different aquatic fungi in freshwater ecosystems.
The Central Andean region is characterized by diverse climate zones with sharp transitions between them. In this work, the area of interest is the South-Central Andes in northwestern Argentina that borders with Bolivia and Chile. The focus is the observation of soil moisture and water vapour with Global Navigation Satellite System (GNSS) remote-sensing methodologies. Because of the rapid temporal and spatial variations of water vapour and moisture circulations, monitoring this part of the hydrological cycle is crucial for understanding the mechanisms that control the local climate. Moreover, GNSS-based techniques have previously shown high potential and are appropriate for further investigation. This study includes both logistic-organization effort and data analysis. As for the prior, three GNSS ground stations were installed in remote locations in northwestern Argentina to acquire observations, where there was no availability of third-party data.
The methodological development for the observation of the climate variables of soil moisture and water vapour is independent and relies on different approaches. The soil-moisture estimation with GNSS reflectometry is an approximation that has demonstrated promising results, but it has yet to be operationally employed. Thus, a more advanced algorithm that exploits more observations from multiple satellite constellations was developed using data from two pilot stations in Germany. Additionally, this algorithm was slightly modified and used in a sea-level measurement campaign. Although the objective of this application is not related to monitoring hydrological parameters, its methodology is based on the same principles and helps to evaluate the core algorithm. On the other hand, water-vapour monitoring with GNSS observations is a well-established technique that is utilized operationally. Hence, the scope of this study is conducting a meteorological analysis by examining the along-the-zenith air-moisture levels and introducing indices related to the azimuthal gradient.
The results of the experiments indicate higher-quality soil moisture observations with the new algorithm. Furthermore, the analysis using the stations in northwestern Argentina illustrates the limits of this technology because of varying soil conditions and shows future research directions. The water-vapour analysis points out the strong influence of the topography on atmospheric moisture circulation and rainfall generation. Moreover, the GNSS time series allows for the identification of seasonal signatures, and the azimuthal-gradient indices permit the detection of main circulation pathways.
The use of alternating current (AC) electrokinetic forces, like dielectrophoresis and AC electroosmosis, as a simple and fast method to immobilize sub-micrometer objects onto nanoelectrode arrays is presented. Due to its medical relevance, the influenza virus is chosen as a model organism. One of the outstanding features is that the immobilization of viral material to the electrodes can be achieved permanently, allowing subsequent handling independently from the electrical setup. Thus, by using merely electric fields, we demonstrate that the need of prior chemical surface modification could become obsolete. The accumulation of viral material over time is observed by fluorescence microscopy. The influences of side effects like electrothermal fluid flow, causing a fluid motion above the electrodes and causing an intensity gradient within the electrode array, are discussed. Due to the improved resolution by combining fluorescence microscopy with deconvolution, it is shown that the viral material is mainly drawn to the electrode edge and to a lesser extent to the electrode surface. Finally, areas of application for this functionalization technique are presented.
Lithium-ion batteries have revolutionized battery technology. However, the scarcity of lithium in nature is driving the search for alternatives. For that reason, sodium-ion batteries have attracted increasing attention in recent years. The main obstacle to their development is the anode as, unlike for lithium-ion batteries, graphite cannot be used due to the inability to form stoichiometrically useful intercalation compounds with sodium. A promising candidate for sodium storage is hard carbon a form of nongraphitisable carbon, that can be synthesized from various precursor materials. Processing of hard carbons is often done by using mechanochemical treatments. Although it is generally accepted and often observed that they can influence the porosity of hard carbons, their effect on battery performance not well understood. Here, the changes in porosity occurring during ball milling are elucidated and related to the properties of hard carbons in sodium storage. Analysis by combined gas physisorption and small angle X-ray scattering shows that porosity changes during ball milling with a significant increase of the open porosity, unsuitable for reversible sodium storage, and decrease of the closed porosity, suitable for reversible sodium storage. While pristine hard carbon can store 58.5 mAh g(-1) in the closed pores, upon 5 h of mechanical treatment in a ball mill it can only store 35.5 mAh g(-1). The obtained results are furthermore pointing towards the disputed "intercalation-adsorption" mechanism.
Graphs play an important role in many areas of Computer Science. In particular, our work is motivated by model-driven software development and by graph databases. For this reason, it is very important to have the means to express and to reason about the properties that a given graph may satisfy. With this aim, in this paper we present a visual logic that allows us to describe graph properties, including navigational properties, i.e., properties about the paths in a graph. The logic is equipped with a deductive tableau method that we have proved to be sound and complete.
Point-of-care and in-vivo bio-diagnostic tools are the current need for the present critical scenarios in the healthcare industry. The past few decades have seen a surge in research activities related to solving the challenges associated with precise on-site bio-sensing. Cutting-edge fiber optic technology enables the interaction of light with functionalized fiber surfaces at remote locations to develop a novel, miniaturized and cost-effective lab on fiber technology for bio-sensing applications. The recent remarkable developments in the field of nanotechnology provide innumerable functionalization methodologies to develop selective bio-recognition elements for label free biosensors. These exceptional methods may be easily integrated with fiber surfaces to provide highly selective light-matter interaction depending on various transduction mechanisms. In the present review, an overview of optical fiber-based biosensors has been provided with focus on physical principles used, along with the functionalization protocols for the detection of various biological analytes to diagnose the disease. The design and performance of these biosensors in terms of operating range, selectivity, response time and limit of detection have been discussed. In the concluding remarks, the challenges associated with these biosensors and the improvement required to develop handheld devices to enable direct target detection have been highlighted.
Die Arbeit „Die Bekämpfung transnationaler Kriminalität im Kontext fragiler Staatlichkeit“ widmet sich dem Phänomen grenzüberschreitend tätiger Akteure der organisierten Kriminalität die den Umstand ausnutzen, dass einige international anerkannte Regierungen nur eine unzureichende Kontrolle über Teile ihres Staatsgebietes ausüben. Es wird untersucht, weshalb der durch die internationale Staatengemeinschaft geschaffene Rechtsrahmen, zur Bekämpfung transnationaler Kriminalitätsphänomene im Kontext dieser fragilen Staaten, nicht oder nur defizitär dazu beiträgt die Kriminalitätsphänomene zu bekämpfen.
Nachdem zunächst erörtert wird, was im Rahmen der Untersuchung unter dem Begriff der transnationalen Kriminalität verstanden wird, wird der internationale Rechtsrahmen zur Bekämpfung anhand von fünf beispielhaft ausgewählten transnationalen Kriminalitätsphänomenen beschrieben. Im darauffolgenden Hauptteil der Untersuchung wird der Frage nachgegangen, weshalb dieser durch die internationale Staatengemeinschaft geschaffene Rechtsrahmen, gerade in fragilen Staaten, kaum einen Beitrag dazu leistet solchen Kriminalitätsphänomenen effektiv zu begegnen. Dabei wird festgestellt, dass die Genese des internationalen Rechtsrahmens zu einem Legitimitätsdefizit desselbigen führt. Auch die mangelhafte Berücksichtigung der speziellen Lebensrealitäten, die in vielen fragilen Staaten vorzufinden sind, wirkt sich negativ auf die Durchsetzbarkeit des internationalen Rechtsrahmens aus. Es wird dargelegt, dass unterschiedlich hohe menschenrechtliche Schutzstandards zu Normenkollisionen bei der internationalen Zusammenarbeit der Staaten führen, insbesondere im Rahmen der internationalen Rechtshilfe. Da gerade fragile Staaten häufig durch eine defizitäre menschenrechtliche Situation gekennzeichnet sind, stellt dies konsolidierte Staaten im Rahmen der Zusammenarbeit mit fragilen Staaten öfters vor Herausforderungen. Schließlich wird aufgezeigt, dass auch die extraterritoriale Jurisdiktion und somit die strafrechtliche Verfolgung transnationaler Straftaten durch Drittstaaten mit rechtlichen und praktischen Problemen einhergeht.
In einem letzten Kapitel der Arbeit wird der Frage nachgegangen, ob nicht ein alternativer Strafverfolgungsmechanismus geschaffen werden sollte, um transnationale Kriminalitätsphänomene zu verfolgen, die aus fragilen Staaten heraus begangen werden und wie ein solch alternativer Strafverfolgungsmechanismus konkret ausgestaltet sein sollte.
Macro-economic assessments of climate impacts lack an analysis of the distribution of daily rainfall, which can resolve both complex societal impact channels and anthropogenically forced changes(1-6). Here, using a global panel of subnational economic output for 1,554 regions worldwide over the past 40 years, we show that economic growth rates are reduced by increases in the number of wet days and in extreme daily rainfall, in addition to responding nonlinearly to the total annual and to the standardized monthly deviations of rainfall. Furthermore, high-income nations and the services and manufacturing sectors are most strongly hindered by both measures of daily rainfall, complementing previous work that emphasized the beneficial effects of additional total annual rainfall in low-income, agriculturally dependent economies(4,7). By assessing the distribution of rainfall at multiple timescales and the effects on different sectors, we uncover channels through which climatic conditions can affect the economy. These results suggest that anthropogenic intensification of daily rainfall extremes(8-10) will have negative global economic consequences that require further assessment by those who wish to evaluate the costs of anthropogenic climate change.
Selection of high-performance lines with respect to traits of interest is a key step in plant breeding. Genomic prediction allows to determine the genomic estimated breeding values of unseen lines for trait of interest using genetic markers, e.g. single-nucleotide polymorphisms (SNPs), and machine learning approaches, which can therefore shorten breeding cycles, referring to genomic selection (GS). Here, we applied GS approaches in two populations of Solanaceous crops, i.e. tomato and pepper, to predict morphometric and colorimetric traits. The traits were measured by using scoring-based conventional descriptors (CDs) as well as by Tomato Analyzer (TA) tool using the longitudinally and latitudinally cut fruit images. The GS performance was assessed in cross-validations of classification-based and regression-based machine learning models for CD and TA traits, respectively. The results showed the usage of TA traits and tag SNPs provide a powerful combination to predict morphology and color-related traits of Solanaceous fruits. The highest predictability of 0.89 was achieved for fruit width in pepper, with an average predictability of 0.69 over all traits. The multi-trait GS models are of slightly better predictability than single-trait models for some colorimetric traits in pepper. While model validation performs poorly on wild tomato accessions, the usage as many as one accession per wild species in the training set can increase the transferability of models to unseen populations for some traits (e.g. fruit shape for which predictability in unseen scenario increased from zero to 0.6). Overall, GS approaches can assist the selection of high-performance Solanaceous fruits in crop breeding.
Modern data analysis tasks often involve control flow statements, such as the iterations in PageRank and K-means. To achieve scalability, developers usually implement these tasks in distributed dataflow systems, such as Spark and Flink. Designers of such systems have to choose between providing imperative or functional control flow constructs to users. Imperative constructs are easier to use, but functional constructs are easier to compile to an efficient dataflow job. We propose Mitos, a system where control flow is both easy to use and efficient. Mitos relies on an intermediate representation based on the static single assignment form. This allows us to abstract away from specific control flow constructs and treat any imperative control flow uniformly both when building the dataflow job and when coordinating the distributed execution.
This study focuses on William Faulkner, whose works explore the demise of the slavery-based Old South during the Civil War in a highly experimental narrative style. Central to this investigation is the analysis of the temporal dimensions of both individual and collective guilt, thus offering a new approach to the often-discussed problem of Faulkner’s portrayal of social decay. The thesis examines how Faulkner re-narrates the legacy of the Old South as a guilt narrative and argues that Faulkner uses guilt in order to corroborate his concept of time and the idea of the continuity of the past. The focus of the analysis is on three of Faulkner’s arguably most important novels: The Sound and the Fury, Absalom, Absalom!, and Go Down, Moses. Each of these novels features a main character deeply overwhelmed by the crimes of the past, whether private, familial, or societal. As a result, guilt is explored both from a domestic as well as a social perspective. In order to show how Faulkner blends past and present by means of guilt, this work examines several methods and motifs borrowed from different fields and genres with which Faulkner narratively negotiates guilt. These include religious notions of original sin, the motif of the ancestral curse prevalent in the Southern Gothic genre, and the psychological concept of trauma. Each of these motifs emphasizes the temporal dimensions of guilt, which are the core of this study, and makes clear that guilt in Faulkner’s work is primarily to be understood as a temporal rather than a moral problem.
A comprehensive theory of child language acquisition requires an evidential base that is representative of the typological diversity present in the world's 7000 or so languages. However, languages are dying at an alarming rate, and the next 50 years represents the last chance we have to document acquisition in many of them. Here, we take stock of the last 45 years of research published in the four main child language acquisition journals: Journal of Child Language, First Language, Language Acquisition and Language Learning and Development. We coded each article for several variables, including (1) participant group (mono vs multilingual), (2) language(s), (3) topic(s) and (4) country of author affiliation, from each journal's inception until the end of 2020. We found that we have at least one article published on around 103 languages, representing approximately 1.5% of the world's languages. The distribution of articles was highly skewed towards English and other well-studied Indo-European languages, with the majority of non-Indo-European languages having just one paper. A majority of the papers focused on studies of monolingual children, although papers did not always explicitly report participant group status. The distribution of topics across language categories was more even. The number of articles published on non-Indo-European languages from countries outside of North America and Europe is increasing; however, this increase is driven by research conducted in relatively wealthy countries. Overall, the vast majority of the research was produced in the Global North. We conclude that, despite a proud history of crosslinguistic research, the goals of the discipline need to be recalibrated before we can lay claim to truly a representative account of child language acquisition.
Assessing the impact of global change on hydrological systems is one of the greatest hydrological challenges of our time. Changes in land cover, land use, and climate have an impact on water quantity, quality, and temporal availability. There is a widespread consensus that, given the far-reaching effects of global change, hydrological systems can no longer be viewed as static in their structure; instead, they must be regarded as entire ecosystems, wherein hydrological processes interact and coevolve with biological, geomorphological, and pedological processes. To accurately predict the hydrological response under the impact of global change, it is essential to understand this complex coevolution. The knowledge of how hydrological processes, in particular the formation of subsurface (preferential) flow paths, evolve within this coevolution and how they feed back to the other processes is still very limited due to a lack of observational data.
At the hillslope scale, this intertwined system of interactions is known as the hillslope feedback cycle. This thesis aims to enhance our understanding of the hillslope feedback cycle by studying the coevolution of hillslope structure and hillslope hydrological response. Using chronosequences of moraines in two glacial forefields developed from siliceous and calcareous glacial till, the four studies shed light on the complex coevolution of hydrological, biological, and structural hillslope properties, as well as subsurface hydrological flow paths over an evolutionary period of 10 millennia in these two contrasting geologies. The findings indicate that the contrasting properties of siliceous and calcareous parent materials lead
to variations in soil structure, permeability, and water storage. As a result, different plant species and vegetation types are favored on siliceous versus calcareous parent material, leading to diverse ecosystems with distinct hydrological dynamics. The siliceous parent material was found to show a higher activity level in driving the coevolution. The soil pH resulting from parent material weathering emerges as a crucial factor, influencing vegetation development, soil formation, and consequently, hydrology. The acidic weathering of the siliceous parent material favored the accumulation of organic matter, increasing the soils’ water storage capacity and attracting acid-loving shrubs, which further promoted organic matter accumulation and ultimately led to podsolization after 10 000 years. Tracer experiments revealed that the subsurface flow path evolution was influenced by soil and vegetation development, and vice versa. Subsurface flow paths changed from vertical, heterogeneous matrix flow to finger-like flow paths over a few hundred years, evolving into macropore flow, water storage, and lateral subsurface flow after several thousand years. The changes in flow paths among younger age classes were driven by weathering processes altering soil structure, as well as by vegetation development and root activity. In the older age
class, the transition to more water storage and lateral flow was attributed to substantial organic matter accumulation and ongoing podsolization. The rapid vertical water transport in the finger-like flow paths, along with the conductive sandy material, contributed to podsolization and thus to the shift in the hillslope hydrological response.
In contrast, the calcareous site possesses a high pH buffering capacity, creating a neutral to basic environment with relatively low accumulation of dead organic matter, resulting in a lower water storage capacity and the establishment of predominantly grass vegetation. The coevolution was found to be less dynamic over the millennia. Similar to the siliceous site, significant changes in subsurface flow paths occurred between the young age classes. However, unlike the siliceous site, the subsurface flow paths at the calcareous site only altered in shape and not in direction. Tracer experiments showed that flow paths changed from vertical, heterogeneous matrix flow to vertical, finger-like flow paths after a few hundred to thousands of years, which was driven by root activities and weathering processes. Despite having a finer soil texture, water storage at the calcareous site was significantly lower than at the siliceous site, and water transport remained primarily rapid and vertical, contributing to the flourishing of grass vegetation.
The studies elucidated that changes in flow paths are predominantly shaped by the characteristics of the parent material and its weathering products, along with their complex interactions with initial water flow paths and vegetation development. Time, on the other hand, was not found to be a primary factor in describing the evolution of the hydrological response. This thesis makes a valuable contribution to closing the gap in the observations of the coevolution of hydrological processes within the hillslope feedback cycle, which is important to improve predictions of hydrological processes in changing landscapes. Furthermore, it emphasizes the importance of interdisciplinary studies in addressing the hydrological challenges arising from global change.
Background:
From birth to young adulthood, health and development of young people are strongly linked to their living situation, including their family's socioeconomic position (SEP) and living environment. The impact of regional characteristics on development in early childhood beyond family SEP has been rarely investigated. This study aimed to identify regional predictors of global developmental delay at school entry taking family SEP into consideration.
Method:
We used representative, population-based data from mandatory school entry examinations of the German federal state of Brandenburg in 2018/2019 with n=22,801 preschool children. By applying binary multilevel models, we hierarchically analyzed the effect of regional deprivation defined by the German Index of Socioeconomic Deprivation (GISD) and rurality operationalized as inverted population density of the children's school district on global developmental delay (GDD) while adjusting for family SEP (low, medium and high)
Results:
Family SEP was significantly and strongly linked to GDD. Children with the highest family SEP showed a lower odds for GDD compared to a medium SEP (female: OR=4.26, male: OR=3.46) and low SEP (female: OR=16.58, male: OR=12.79). Furthermore, we discovered a smaller, but additional and independent effect of regional socioeconomic deprivation on GDD, with a higher odds for children from a more deprived school district (female: OR=1.35, male: OR=1.20). However, rurality did not show a significant link to GDD in preschool children beyond family SEP and regional deprivation.
Conclusion:
Family SEP and regional deprivation are risk factors for child development and of particular interest to promote health of children in early childhood and over the life course.
Boreal forests of Siberia play a relevant role in the global carbon cycle. However, global warming threatens the existence of summergreen larch-dominated ecosystems, likely enabling a transition to evergreen tree taxa with deeper active layers. Complex permafrost-vegetation interactions make it uncertain whether these ecosystems could develop into a carbon source rather than continuing atmospheric carbon sequestration under global warming. Consequently, shedding light on the role of current and future active layer dynamics and the feedbacks with the apparent tree species is crucial to predict boreal forest transition dynamics and thus for aboveground forest biomass and carbon stock developments. Hence, we established a coupled model version amalgamating a one-dimensional permafrost multilayer forest land-surface model (CryoGrid) with LAVESI, an individual-based and spatially explicit forest model for larch species (Larix Mill.), extended for this study by including other relevant Siberian forest species and explicit terrain. <br /> Following parameterization, we ran simulations with the coupled version to the near future to 2030 with a mild climate-warming scenario. We focus on three regions covering a gradient of summergreen forests in the east at Spasskaya Pad, mixed summergreen-evergreen forests close to Nyurba, and the warmest area at Lake Khamra in the southeast of Yakutia, Russia. Coupled simulations were run with the newly implemented boreal forest species and compared to runs allowing only one species at a time, as well as to simulations using just LAVESI. Results reveal that the coupled version corrects for overestimation of active layer thickness (ALT) and soil moisture, and large differences in established forests are simulated. We conclude that the coupled version can simulate the complex environment of eastern Siberia by reproducing vegetation patterns, making it an excellent tool to disentangle processes driving boreal forest dynamics.
The CH2Cl2/MeOH (1:1) extract of Zanthoxylum holstzianum stem bark showed good antiplasmodial activity (IC50 2.5 +/- 0.3 and 2.6 +/- 0.3 mu g/mL against the W2 and D6 strains of Plasmodium falciparum, respectively). From the extract five benzophenanthridine alkaloids [8-acetonyldihydrochelerythrine (1), nitidine (2), dihydrochelerythine (3), norchelerythrine (5), arnottianamide (8)]; a 2-quinolone alkaloid [N-methylflindersine (4)]; a lignan [4,4 '-dihydroxy-3,3 '-dimethoxylignan-9,9 '-diyl diacetate (7)] and a dimer of a benzophenanthridine and 2-quinoline [holstzianoquinoline (6)] were isolated. The CH2Cl2/MeOH (1:1) extract of the root bark afforded 1, 3-6, 8, chelerythridimerine (9) and 9-demethyloxychelerythrine (10). Holstzianoquinoline (6) is new, and is the second dimer linked by a C-C bond of a benzophenanthridine and a 2-quinoline reported thus far. The compounds were identified based on spectroscopic evidence. Amongst five compounds (1-5) tested against two strains of P. falciparum, nitidine (IC50 0.11 +/- 0.01 mu g/mL against W2 and D6 strains) and norchelerythrine (IC50 value of 0.15 +/- 0.01 mu g/mL against D6 strain) were the most active.
Flexural strike-slip basins
(2021)
Strike-slip faults are classically associated with pull-apart basins where continental crust is thinned between two laterally offset fault segments. We propose a subsidence mechanism to explain the formation of a new type of basin where no substantial segment offset or synstrike-slip thinning is observed. Such "flexural strike-slip basins" form due to a sediment load creating accommodation space by bending the lithosphere. We use a two-way coupling between the geodynamic code ASPECT and surface-processes code FastScape to show that flexural strike-slip basins emerge if sediment is deposited on thin lithosphere close to a strike slip fault. These conditions were met at the Andaman Basin Central fault (Andaman Sea, Indian Ocean), where seismic reflection data provide evidence of a laterally extensive flexural basin with a depocenter located parallel to the strike-slip fault trace.
Pre-service physics teachers often do not recognise the relevance for their future career in their university content knowledge courses. A lower perceived relevance can, however, have a negative effect on their motivation and on their academic success. Several intervention studies have been undertaken with the goal to increase this perceived relevance. A previous study shows that conceptual physics problems used in university physics courses are perceived by pre-service physics teachers as more relevant for their future career than regular, quantitative problems. It is however not clear, what the students' meaning of the construct 'relevance' is: what makes a problem more relevant to them than another problem? To answer this question, N = 7 pre-service teachers were interviewed using the repertory grid technique, based on the personal construct theory. Nine physics problems were discussed with regards to their perceived relevance and with regards to problem properties that distinguish these problems from each other. We are able to identify six problem properties that have a positive influence on the perceived relevance. Physics problems that are based on these properties should therefore potentially have a higher perceived relevance, which can have a positive effect on the motivation of the pre-service teachers who solve these problems.
A synthesis route to controlled and dynamic single polymer chain folding is reported. Sequence-controlled macromolecules containing precisely located selenol moieties within a polymer chain are synthesized. Oxidation of selenol functionalities lead to diselenide bridges and induces controlled intramolecular crosslinking to generate single chain collapse. The cyclization process is successfully characterized by SEC as well as by H-1 NMR and 2D HSQC NMR spectroscopies. In order to gain insight on the molecular level to reveal the degree of structural control, the folded polymers are transformed into folded molecular brushes that are known to be visualizable as single molecule structures by AFM. The "grafting onto" approach is performed by using triazolinedione-diene reaction to graft the side chain polymers. A series of folded molecular brushes as well as the corresponding linear controls are synthesized. AFM visualization is proving the cyclization of the folded backbone by showing globular objects, where non-folded brushes show typical worm-like structures. (C) 2019 The Authors. Journal of Polymer Science published by Wiley Periodicals, Inc.
A synthesis route to controlled and dynamic single polymer chain folding is reported. Sequence-controlled macromolecules containing precisely located selenol moieties within a polymer chain are synthesized. Oxidation of selenol functionalities lead to diselenide bridges and induces controlled intramolecular crosslinking to generate single chain collapse. The cyclization process is successfully characterized by SEC as well as by H-1 NMR and 2D HSQC NMR spectroscopies. In order to gain insight on the molecular level to reveal the degree of structural control, the folded polymers are transformed into folded molecular brushes that are known to be visualizable as single molecule structures by AFM. The "grafting onto" approach is performed by using triazolinedione-diene reaction to graft the side chain polymers. A series of folded molecular brushes as well as the corresponding linear controls are synthesized. AFM visualization is proving the cyclization of the folded backbone by showing globular objects, where non-folded brushes show typical worm-like structures. (C) 2019 The Authors. Journal of Polymer Science published by Wiley Periodicals, Inc.
Previous studies have explored the consequences of flood events for exposed households and companies by focusing on single flood events. Less is known about the consequences of experiencing repeated flood events for the resilience of households and companies. In this paper, we therefore explore how multiple floods experience affects the resilience of exposed households and companies. Resilience was made operational through individual appraisals of households and companies' ability to withstand and recover from material as well as health and psychological impacts of the 2013 flood in Germany. The paper is based on three different datasets including more than 2000 households and 300 companies that were affected by the 2013 flood. The surveys revealed that the resilience of households seems to increase, but only with regard to their subjectively appraised ability to withstand impacts on mobile goods and equipment (e.g., cars, TV, and radios). In regard to the ability of households to withstand overall financial consequences of repetitive floods, evidence for nonlinear (quadratic) trends can be found. With regard to psychological and health-related consequences, the findings are mixed but provide tentative evidence for eroding resilience among households. Companies' resilience increased with respect to material assets but appears to decrease with respect to ability to recover. We conclude by arguing that clear and operational definitions of resilience are required so that evidence-based resilience baselines can be established to assess whether resilience is eroding or improving over time.
Previous studies have explored the consequences of flood events for exposed households and companies by focusing on single flood events. Less is known about the consequences of experiencing repeated flood events for the resilience of households and companies. In this paper, we therefore explore how multiple floods experience affects the resilience of exposed households and companies. Resilience was made operational through individual appraisals of households and companies' ability to withstand and recover from material as well as health and psychological impacts of the 2013 flood in Germany. The paper is based on three different datasets including more than 2000 households and 300 companies that were affected by the 2013 flood. The surveys revealed that the resilience of households seems to increase, but only with regard to their subjectively appraised ability to withstand impacts on mobile goods and equipment (e.g., cars, TV, and radios). In regard to the ability of households to withstand overall financial consequences of repetitive floods, evidence for nonlinear (quadratic) trends can be found. With regard to psychological and health-related consequences, the findings are mixed but provide tentative evidence for eroding resilience among households. Companies' resilience increased with respect to material assets but appears to decrease with respect to ability to recover. We conclude by arguing that clear and operational definitions of resilience are required so that evidence-based resilience baselines can be established to assess whether resilience is eroding or improving over time.
Adverse environmental conditions are detrimental to plant growth and development. Acclimation to abiotic stress conditions involves activation of signaling pathways which often results in changes in gene expression via networks of transcription factors (TFs). Mediator is a highly conserved co-regulator complex and an essential component of the transcriptional machinery in eukaryotes. Some Mediator subunits have been implicated in stress-responsive signaling pathways; however, much remains unknown regarding the role of plant Mediator in abiotic stress responses. Here, we use RNA-seq to analyze the transcriptional response of Arabidopsis thaliana to heat, cold and salt stress conditions. We identify a set of common abiotic stress regulons and describe the sequential and combinatorial nature of TFs involved in their transcriptional regulation. Furthermore, we identify stress-specific roles for the Mediator subunits MED9, MED16, MED18 and CDK8, and putative TFs connecting them to different stress signaling pathways. Our data also indicate different modes of action for subunits or modules of Mediator at the same gene loci, including a co-repressor function for MED16 prior to stress. These results illuminate a poorly understood but important player in the transcriptional response of plants to abiotic stress and identify target genes and mechanisms as a prelude to further biochemical characterization.
Being born large for gestational age is associated with increased global placental DNA methylation
(2020)
Being born small (SGA) or large for gestational age (LGA) is associated with adverse birth outcomes and metabolic diseases in later life of the offspring. It is known that aberrations in growth during gestation are related to altered placental function. Placental function is regulated by epigenetic mechanisms such as DNA methylation. Several studies in recent years have demonstrated associations between altered patterns of DNA methylation and adverse birth outcomes. However, larger studies that reliably investigated global DNA methylation are lacking. The aim of this study was to characterize global placental DNA methylation in relationship to size for gestational age. Global DNA methylation was assessed in 1023 placental samples by LC-MS/MS. LGA offspring displayed significantly higher global placental DNA methylation compared to appropriate for gestational age (AGA; p<0.001). ANCOVA analyses adjusted for known factors impacting on DNA methylation demonstrated an independent association between placental global DNA methylation and LGA births (p<0.001). Tertile stratification according to global placental DNA methylation levels revealed a significantly higher frequency of LGA births in the third tertile. Furthermore, a multiple logistic regression analysis corrected for known factors influencing birth weight highlighted an independent positive association between global placental DNA methylation and the frequency of LGA births (p=0.001).
Being born large for gestational age is associated with increased global placental DNA methylation
(2020)
Being born small (SGA) or large for gestational age (LGA) is associated with adverse birth outcomes and metabolic diseases in later life of the offspring. It is known that aberrations in growth during gestation are related to altered placental function. Placental function is regulated by epigenetic mechanisms such as DNA methylation. Several studies in recent years have demonstrated associations between altered patterns of DNA methylation and adverse birth outcomes. However, larger studies that reliably investigated global DNA methylation are lacking. The aim of this study was to characterize global placental DNA methylation in relationship to size for gestational age. Global DNA methylation was assessed in 1023 placental samples by LC-MS/MS. LGA offspring displayed significantly higher global placental DNA methylation compared to appropriate for gestational age (AGA; p<0.001). ANCOVA analyses adjusted for known factors impacting on DNA methylation demonstrated an independent association between placental global DNA methylation and LGA births (p<0.001). Tertile stratification according to global placental DNA methylation levels revealed a significantly higher frequency of LGA births in the third tertile. Furthermore, a multiple logistic regression analysis corrected for known factors influencing birth weight highlighted an independent positive association between global placental DNA methylation and the frequency of LGA births (p=0.001).
The hyperthermal events of the Cenozoic, including the Paleocene-Eocene Thermal Maximum, provide an opportunity to investigate the potential effects of climate warming on marine ecosystems. Here, we examine the shallow benthic marine communities preserved in the late Cretaceous to Eocene strata on the Gulf Coastal Plain (United States). In stark contrast to the ecological shifts following the end-Cretaceous mass extinction, our data show that the early Cenozoic hyperthermals did not have a long-term impact on the generic diversity nor composition of the Gulf Coastal Plain molluscan communities. We propose that these communities were resilient to climate change because molluscs are better adapted to high temperatures than other taxa, as demonstrated by their physiology and evolutionary history. In terms of resilience, these communities differ from other shallow-water carbonate ecosystems, such as reef communities, which record significant changes during the early Cenozoic hyperthermals. These data highlight the strikingly different responses of community types, i.e., the almost imperceptible response of molluscs versus the marked turnover of foraminifera and reef faunas. The impact on molluscan communities may have been low because detrimental conditions did not devastate the entire Gulf Coastal Plain, allowing molluscs to rapidly recolonise vacated areas once harsh environmental conditions ameliorated.
The hyperthermal events of the Cenozoic, including the Paleocene-Eocene Thermal Maximum, provide an opportunity to investigate the potential effects of climate warming on marine ecosystems. Here, we examine the shallow benthic marine communities preserved in the late Cretaceous to Eocene strata on the Gulf Coastal Plain (United States). In stark contrast to the ecological shifts following the end-Cretaceous mass extinction, our data show that the early Cenozoic hyperthermals did not have a long-term impact on the generic diversity nor composition of the Gulf Coastal Plain molluscan communities. We propose that these communities were resilient to climate change because molluscs are better adapted to high temperatures than other taxa, as demonstrated by their physiology and evolutionary history. In terms of resilience, these communities differ from other shallow-water carbonate ecosystems, such as reef communities, which record significant changes during the early Cenozoic hyperthermals. These data highlight the strikingly different responses of community types, i.e., the almost imperceptible response of molluscs versus the marked turnover of foraminifera and reef faunas. The impact on molluscan communities may have been low because detrimental conditions did not devastate the entire Gulf Coastal Plain, allowing molluscs to rapidly recolonise vacated areas once harsh environmental conditions ameliorated.
Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1(-/-) mice results in replication of HSV-1 and Asah1(-/-) mice die soon after systemic or intravaginal inoculation. The treatment of macrophages with sphingosine enhancing compounds blocks HSV-1 propagation, suggesting a therapeutic potential of this pathway. In conclusion, aCDase loads ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol.
Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1(-/-) mice results in replication of HSV-1 and Asah1(-/-) mice die soon after systemic or intravaginal inoculation. The treatment of macrophages with sphingosine enhancing compounds blocks HSV-1 propagation, suggesting a therapeutic potential of this pathway. In conclusion, aCDase loads ILVs with sphingosine, which prevents HSV-1 capsids from penetrating into the cytosol.
Groundwater is the biggest single source of high-quality freshwater worldwide, which is also continuously threatened by the changing climate. In this paper, we investigate the response of the regional groundwater system to climate change under three global warming levels (1.5, 2, and 3 ∘C) in a central German basin (Nägelstedt). This investigation is conducted by deploying an integrated modeling workflow that consists of a mesoscale hydrologic model (mHM) and a fully distributed groundwater model, OpenGeoSys (OGS). mHM is forced with climate simulations of five general circulation models under three representative concentration pathways. The diffuse recharges estimated by mHM are used as boundary forcings to the OGS groundwater model to compute changes in groundwater levels and travel time distributions. Simulation results indicate that groundwater recharges and levels are expected to increase slightly under future climate scenarios. Meanwhile, the mean travel time is expected to decrease compared to the historical average. However, the ensemble simulations do not all agree on the sign of relative change. Changes in mean travel time exhibit a larger variability than those in groundwater levels. The ensemble simulations do not show a systematic relationship between the projected change (in both groundwater levels and travel times) and the warming level, but they indicate an increased variability in projected changes with adjusting the enhanced warming level from 1.5 to 3 ∘C. Correspondingly, it is highly recommended to restrain the trend of global warming.
Groundwater is the biggest single source of high-quality freshwater worldwide, which is also continuously threatened by the changing climate. In this paper, we investigate the response of the regional groundwater system to climate change under three global warming levels (1.5, 2, and 3 ∘C) in a central German basin (Nägelstedt). This investigation is conducted by deploying an integrated modeling workflow that consists of a mesoscale hydrologic model (mHM) and a fully distributed groundwater model, OpenGeoSys (OGS). mHM is forced with climate simulations of five general circulation models under three representative concentration pathways. The diffuse recharges estimated by mHM are used as boundary forcings to the OGS groundwater model to compute changes in groundwater levels and travel time distributions. Simulation results indicate that groundwater recharges and levels are expected to increase slightly under future climate scenarios. Meanwhile, the mean travel time is expected to decrease compared to the historical average. However, the ensemble simulations do not all agree on the sign of relative change. Changes in mean travel time exhibit a larger variability than those in groundwater levels. The ensemble simulations do not show a systematic relationship between the projected change (in both groundwater levels and travel times) and the warming level, but they indicate an increased variability in projected changes with adjusting the enhanced warming level from 1.5 to 3 ∘C. Correspondingly, it is highly recommended to restrain the trend of global warming.
Monocytes and macrophages are key players in maintaining immune homeostasis. Identifying strategies to manipulate their functions via gene delivery is thus of great interest for immunological research and biomedical applications. We set out to establish conditions for mRNA transfection in hard-to-transfect primary human monocytes and monocyte-derived macrophages due to the great potential of gene expression from in vitro transcribed mRNA for modulating cell phenotypes. mRNA doses, nucleotide modifications, and different carriers were systematically explored in order to optimize high mRNA transfer rates while minimizing cell stress and immune activation. We selected three commercially available mRNA transfection reagents including liposome and polymer-based formulations, covering different application spectra. Our results demonstrate that liposomal reagents can particularly combine high gene transfer rates with only moderate immune cell activation. For the latter, use of specific nucleotide modifications proved essential. In addition to improving efficacy of gene transfer, our findings address discrete aspects of innate immune activation using cytokine and surface marker expression, as well as cell viability as key readouts to judge overall transfection efficiency. The impact of this study goes beyond optimizing transfection conditions for immune cells, by providing a framework for assessing new gene carrier systems for monocyte and macrophage, tailored to specific applications.
Monocytes and macrophages are key players in maintaining immune homeostasis. Identifying strategies to manipulate their functions via gene delivery is thus of great interest for immunological research and biomedical applications. We set out to establish conditions for mRNA transfection in hard-to-transfect primary human monocytes and monocyte-derived macrophages due to the great potential of gene expression from in vitro transcribed mRNA for modulating cell phenotypes. mRNA doses, nucleotide modifications, and different carriers were systematically explored in order to optimize high mRNA transfer rates while minimizing cell stress and immune activation. We selected three commercially available mRNA transfection reagents including liposome and polymer-based formulations, covering different application spectra. Our results demonstrate that liposomal reagents can particularly combine high gene transfer rates with only moderate immune cell activation. For the latter, use of specific nucleotide modifications proved essential. In addition to improving efficacy of gene transfer, our findings address discrete aspects of innate immune activation using cytokine and surface marker expression, as well as cell viability as key readouts to judge overall transfection efficiency. The impact of this study goes beyond optimizing transfection conditions for immune cells, by providing a framework for assessing new gene carrier systems for monocyte and macrophage, tailored to specific applications.
Experimental studies have reported on the anti-inflammatory properties of polyphenols. However, results from epidemiological investigations have been inconsistent and especially studies using biomarkers for assessment of polyphenol intake have been scant. We aimed to characterise the association between plasma concentrations of thirty-five polyphenol compounds and low-grade systemic inflammation state as measured by high-sensitivity C-reactive protein (hsCRP). A cross-sectional data analysis was performed based on 315 participants in the European Prospective Investigation into Cancer and Nutrition cohort with available measurements of plasma polyphenols and hsCRP. In logistic regression analysis, the OR and 95 % CI of elevated serum hsCRP (>3 mg/l) were calculated within quartiles and per standard deviation higher level of plasma polyphenol concentrations. In a multivariable-adjusted model, the sum of plasma concentrations of all polyphenols measured (per standard deviation) was associated with 29 (95 % CI 50, 1) % lower odds of elevated hsCRP. In the class of flavonoids, daidzein was inversely associated with elevated hsCRP (OR 0 center dot 66, 95 % CI 0 center dot 46, 0 center dot 96). Among phenolic acids, statistically significant associations were observed for 3,5-dihydroxyphenylpropionic acid (OR 0 center dot 58, 95 % CI 0 center dot 39, 0 center dot 86), 3,4-dihydroxyphenylpropionic acid (OR 0 center dot 63, 95 % CI 0 center dot 46, 0 center dot 87), ferulic acid (OR 0 center dot 65, 95 % CI 0 center dot 44, 0 center dot 96) and caffeic acid (OR 0 center dot 69, 95 % CI 0 center dot 51, 0 center dot 93). The odds of elevated hsCRP were significantly reduced for hydroxytyrosol (OR 0 center dot 67, 95 % CI 0 center dot 48, 0 center dot 93). The present study showed that polyphenol biomarkers are associated with lower odds of elevated hsCRP. Whether diet rich in bioactive polyphenol compounds could be an effective strategy to prevent or modulate deleterious health effects of inflammation should be addressed by further well-powered longitudinal studies.
Experimental studies have reported on the anti-inflammatory properties of polyphenols. However, results from epidemiological investigations have been inconsistent and especially studies using biomarkers for assessment of polyphenol intake have been scant. We aimed to characterise the association between plasma concentrations of thirty-five polyphenol compounds and low-grade systemic inflammation state as measured by high-sensitivity C-reactive protein (hsCRP). A cross-sectional data analysis was performed based on 315 participants in the European Prospective Investigation into Cancer and Nutrition cohort with available measurements of plasma polyphenols and hsCRP. In logistic regression analysis, the OR and 95 % CI of elevated serum hsCRP (>3 mg/l) were calculated within quartiles and per standard deviation higher level of plasma polyphenol concentrations. In a multivariable-adjusted model, the sum of plasma concentrations of all polyphenols measured (per standard deviation) was associated with 29 (95 % CI 50, 1) % lower odds of elevated hsCRP. In the class of flavonoids, daidzein was inversely associated with elevated hsCRP (OR 0 center dot 66, 95 % CI 0 center dot 46, 0 center dot 96). Among phenolic acids, statistically significant associations were observed for 3,5-dihydroxyphenylpropionic acid (OR 0 center dot 58, 95 % CI 0 center dot 39, 0 center dot 86), 3,4-dihydroxyphenylpropionic acid (OR 0 center dot 63, 95 % CI 0 center dot 46, 0 center dot 87), ferulic acid (OR 0 center dot 65, 95 % CI 0 center dot 44, 0 center dot 96) and caffeic acid (OR 0 center dot 69, 95 % CI 0 center dot 51, 0 center dot 93). The odds of elevated hsCRP were significantly reduced for hydroxytyrosol (OR 0 center dot 67, 95 % CI 0 center dot 48, 0 center dot 93). The present study showed that polyphenol biomarkers are associated with lower odds of elevated hsCRP. Whether diet rich in bioactive polyphenol compounds could be an effective strategy to prevent or modulate deleterious health effects of inflammation should be addressed by further well-powered longitudinal studies.
Background: Dietary protein restriction is emerging as an alternative approach to treat obesity and glucose intolerance because it markedly increases plasma fibroblast growth factor 21 (FGF21) concentrations. Similarly, dietary restriction of methionine is known to mimic metabolic effects of energy and protein restriction with FGF21 as a required mechanism. However, dietary protein has been shown to be required for normal bone growth, though there is conflicting evidence as to the influence of dietary protein restriction on bone remodeling. The purpose of the current study was to evaluate the effect of dietary protein and methionine restriction on bone in lean and obese mice, and clarify whether FGF21 and general control nonderepressible 2 (GCN2) kinase, that are part of a novel endocrine pathway implicated in the detection of protein restriction, influence the effect of dietary protein restriction on bone.
Methods: Adult wild-type (WT) or Fgf21 KO mice were fed a normal protein (18 kcal%; CON) or low protein (4 kcal%; LP) diet for 2 or 27 weeks. In addition, adult WT or Gcn2 KO mice were fed a CON or LP diet for 27 weeks. Young New Zealand obese (NZO) mice were placed on high-fat diets that provided protein at control (16 kcal%; CON), low levels (4 kcal%) in a high-carbohydrate (LP/HC) or high-fat (LP/HF) regimen, or on high-fat diets (protein, 16 kcal%) that provided methionine at control (0.86%; CON-MR) or low levels (0.17%; MR) for up to 9 weeks. Long bones from the hind limbs of these mice were collected and evaluated with micro-computed tomography (mu CT) for changes in trabecular and cortical architecture and mass.
Results: In WT mice the 27-week LP diet significantly reduced cortical bone, and this effect was enhanced by deletion of Fgf21 but not Gcn2. This decrease in bone did not appear after 2 weeks on the LP diet. In addition, Fgf21 KO mice had significantly less bone than their WT counterparts. In obese NZO mice dietary protein and methionine restriction altered bone architecture. The changes were mediated by FGF21 due to methionine restriction in the presence of cystine, which did not increase plasma FGF21 levels and did not affect bone architecture.
Conclusions: This study provides direct evidence of a reduction in bone following long-term dietary protein restriction in a mouse model, effects that appear to be mediated by FGF21.
Background: Dietary protein restriction is emerging as an alternative approach to treat obesity and glucose intolerance because it markedly increases plasma fibroblast growth factor 21 (FGF21) concentrations. Similarly, dietary restriction of methionine is known to mimic metabolic effects of energy and protein restriction with FGF21 as a required mechanism. However, dietary protein has been shown to be required for normal bone growth, though there is conflicting evidence as to the influence of dietary protein restriction on bone remodeling. The purpose of the current study was to evaluate the effect of dietary protein and methionine restriction on bone in lean and obese mice, and clarify whether FGF21 and general control nonderepressible 2 (GCN2) kinase, that are part of a novel endocrine pathway implicated in the detection of protein restriction, influence the effect of dietary protein restriction on bone.
Methods: Adult wild-type (WT) or Fgf21 KO mice were fed a normal protein (18 kcal%; CON) or low protein (4 kcal%; LP) diet for 2 or 27 weeks. In addition, adult WT or Gcn2 KO mice were fed a CON or LP diet for 27 weeks. Young New Zealand obese (NZO) mice were placed on high-fat diets that provided protein at control (16 kcal%; CON), low levels (4 kcal%) in a high-carbohydrate (LP/HC) or high-fat (LP/HF) regimen, or on high-fat diets (protein, 16 kcal%) that provided methionine at control (0.86%; CON-MR) or low levels (0.17%; MR) for up to 9 weeks. Long bones from the hind limbs of these mice were collected and evaluated with micro-computed tomography (mu CT) for changes in trabecular and cortical architecture and mass.
Results: In WT mice the 27-week LP diet significantly reduced cortical bone, and this effect was enhanced by deletion of Fgf21 but not Gcn2. This decrease in bone did not appear after 2 weeks on the LP diet. In addition, Fgf21 KO mice had significantly less bone than their WT counterparts. In obese NZO mice dietary protein and methionine restriction altered bone architecture. The changes were mediated by FGF21 due to methionine restriction in the presence of cystine, which did not increase plasma FGF21 levels and did not affect bone architecture.
Conclusions: This study provides direct evidence of a reduction in bone following long-term dietary protein restriction in a mouse model, effects that appear to be mediated by FGF21.
Inhibition of acid sphingomyelinase (ASM), a lysosomal enzyme that catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine, may serve as an investigational tool or a therapeutic intervention to control many diseases. Specific ASM inhibitors are currently not sufficiently characterized. Here, we found that 1-aminodecylidene bis-phosphonic acid (ARC39) specifically and efficiently (>90%) inhibits both lysosomal and secretory ASM in vitro. Results from investigating sphingomyelin phosphodiesterase 1 (SMPD1/Smpd1) mRNA and ASM protein levels suggested that ARC39 directly inhibits ASM's catalytic activity in cultured cells, a mechanism that differs from that of functional inhibitors of ASM. We further provide evidence that ARC39 dose- and time-dependently inhibits lysosomal ASM in intact cells, and we show that ARC39 also reduces platelet- and ASM-promoted adhesion of tumor cells. The observed toxicity of ARC39 is low at concentrations relevant for ASM inhibition in vitro, and it does not strongly alter the lysosomal compartment or induce phospholipidosis in vitro. When applied intraperitoneally in vivo, even subtoxic high doses administered short-term induced sphingomyelin accumulation only locally in the peritoneal lavage without significant accumulation in plasma, liver, spleen, or brain. These findings require further investigation with other possible chemical modifications. In conclusion, our results indicate that ARC39 potently and selectively inhibits ASM in vitro and highlight the need for developing compounds that can reach tissue concentrations sufficient for ASM inhibition in vivo.
Inhibition of acid sphingomyelinase (ASM), a lysosomal enzyme that catalyzes the hydrolysis of sphingomyelin into ceramide and phosphorylcholine, may serve as an investigational tool or a therapeutic intervention to control many diseases. Specific ASM inhibitors are currently not sufficiently characterized. Here, we found that 1-aminodecylidene bis-phosphonic acid (ARC39) specifically and efficiently (>90%) inhibits both lysosomal and secretory ASM in vitro. Results from investigating sphingomyelin phosphodiesterase 1 (SMPD1/Smpd1) mRNA and ASM protein levels suggested that ARC39 directly inhibits ASM's catalytic activity in cultured cells, a mechanism that differs from that of functional inhibitors of ASM. We further provide evidence that ARC39 dose- and time-dependently inhibits lysosomal ASM in intact cells, and we show that ARC39 also reduces platelet- and ASM-promoted adhesion of tumor cells. The observed toxicity of ARC39 is low at concentrations relevant for ASM inhibition in vitro, and it does not strongly alter the lysosomal compartment or induce phospholipidosis in vitro. When applied intraperitoneally in vivo, even subtoxic high doses administered short-term induced sphingomyelin accumulation only locally in the peritoneal lavage without significant accumulation in plasma, liver, spleen, or brain. These findings require further investigation with other possible chemical modifications. In conclusion, our results indicate that ARC39 potently and selectively inhibits ASM in vitro and highlight the need for developing compounds that can reach tissue concentrations sufficient for ASM inhibition in vivo.
In nature, plants are often subjected to periods of recurrent environmental stress that can strongly affect their development and productivity. To cope with these conditions, plants can remember a previous stress, which allows them to respond more efficiently to a subsequent stress, a phenomenon known as priming. This ability can be maintained at the somatic level for a few days or weeks after the stress is perceived, suggesting that plants can store information of a past stress during this recovery phase. While the immediate responses to a single stress event have been extensively studied, knowledge on priming effects and how stress memory is stored is still scarce. At the molecular level, memory of a past condition often involves changes in chromatin structure and organization, which may be maintained independently from transcription. In this review, we will summarize the most recent developments in the field and discuss how different levels of chromatin regulation contribute to priming and plant abiotic stress memory.
Regular consumption of fruits and vegetables, which is related to high plasma levels of lipid-soluble micro-nutrients such as carotenoids and tocopherols, is linked to lower incidences of various age-related diseases. Differences in lipid-soluble micronutrient blood concentrations seem to be associated with age. Our retrospective analysis included men and women aged 22-37 and 60-85 years from the Berlin Aging Study II. Participants with simultaneously available plasma samples and dietary data were included (n = 1973). Differences between young and old groups were found for plasma lycopene, alpha-carotene, alpha-tocopherol, beta-cryptoxanthin (only in women), and gamma-tocopherol (only in men). beta-Carotene, retinol and lutein/zeaxanthin did not differ between young and old participants regardless of the sex. We found significant associations for lycopene, alpha-carotene (both inverse), alpha-tocopherol, gamma-tocopherol, and beta-carotene (all positive) with age. Adjusting for BMI, smoking status, season, cholesterol and dietary intake confirmed these associations, except for beta-carotene. These micronutrients are important antioxidants and associated with lower incidence of age-related diseases, therefore it is important to understand the underlying mechanisms in order to implement dietary strategies for the prevention of age-related diseases. To explain the lower lycopene and alpha-carotene concentration in older subjects, bioavailability studies in older participants are necessary.
Regular consumption of fruits and vegetables, which is related to high plasma levels of lipid-soluble micro-nutrients such as carotenoids and tocopherols, is linked to lower incidences of various age-related diseases. Differences in lipid-soluble micronutrient blood concentrations seem to be associated with age. Our retrospective analysis included men and women aged 22-37 and 60-85 years from the Berlin Aging Study II. Participants with simultaneously available plasma samples and dietary data were included (n = 1973). Differences between young and old groups were found for plasma lycopene, alpha-carotene, alpha-tocopherol, beta-cryptoxanthin (only in women), and gamma-tocopherol (only in men). beta-Carotene, retinol and lutein/zeaxanthin did not differ between young and old participants regardless of the sex. We found significant associations for lycopene, alpha-carotene (both inverse), alpha-tocopherol, gamma-tocopherol, and beta-carotene (all positive) with age. Adjusting for BMI, smoking status, season, cholesterol and dietary intake confirmed these associations, except for beta-carotene. These micronutrients are important antioxidants and associated with lower incidence of age-related diseases, therefore it is important to understand the underlying mechanisms in order to implement dietary strategies for the prevention of age-related diseases. To explain the lower lycopene and alpha-carotene concentration in older subjects, bioavailability studies in older participants are necessary.
Bacterial chemotaxis-a fundamental example of directional navigation in the living world-is key to many biological processes, including the spreading of bacterial infections. Many bacterial species were recently reported to exhibit several distinct swimming modes-the flagella may, for example, push the cell body or wrap around it. How do the different run modes shape the chemotaxis strategy of a multimode swimmer? Here, we investigate chemotactic motion of the soil bacterium Pseudomonas putida as a model organism. By simultaneously tracking the position of the cell body and the configuration of its flagella, we demonstrate that individual run modes show different chemotactic responses in nutrition gradients and, thus, constitute distinct behavioral states. On the basis of an active particle model, we demonstrate that switching between multiple run states that differ in their speed and responsiveness provides the basis for robust and efficient chemotaxis in complex natural habitats.
Metagenomic sequencing has revolutionised our knowledge of virus diversity, with new virus sequences being reported faster than ever before. However, virus discovery from metagenomic sequencing usually depends on detectable homology: without a sufficiently close relative, so-called ‘dark’ virus sequences remain unrecognisable. An alternative approach is to use virus-identification methods that do not depend on detecting homology, such as virus recognition by host antiviral immunity. For example, virus-derived small RNAs have previously been used to propose ‘dark’ virus sequences associated with the Drosophilidae (Diptera). Here, we combine published Drosophila data with a comprehensive search of transcriptomic sequences and selected meta-transcriptomic datasets to identify a completely new lineage of segmented positive-sense single-stranded RNA viruses that we provisionally refer to as the Quenyaviruses. Each of the five segments contains a single open reading frame, with most encoding proteins showing no detectable similarity to characterised viruses, and one sharing a small number of residues with the RNA-dependent RNA polymerases of single- and double-stranded RNA viruses. Using these sequences, we identify close relatives in approximately 20 arthropods, including insects, crustaceans, spiders, and a myriapod. Using a more conserved sequence from the putative polymerase, we further identify relatives in meta-transcriptomic datasets from gut, gill, and lung tissues of vertebrates, reflecting infections of vertebrates or of their associated parasites. Our data illustrate the utility of small RNAs to detect viruses with limited sequence conservation, and provide robust evidence for a new deeply divergent and phylogenetically distinct RNA virus lineage.
Metagenomic sequencing has revolutionised our knowledge of virus diversity, with new virus sequences being reported faster than ever before. However, virus discovery from metagenomic sequencing usually depends on detectable homology: without a sufficiently close relative, so-called ‘dark’ virus sequences remain unrecognisable. An alternative approach is to use virus-identification methods that do not depend on detecting homology, such as virus recognition by host antiviral immunity. For example, virus-derived small RNAs have previously been used to propose ‘dark’ virus sequences associated with the Drosophilidae (Diptera). Here, we combine published Drosophila data with a comprehensive search of transcriptomic sequences and selected meta-transcriptomic datasets to identify a completely new lineage of segmented positive-sense single-stranded RNA viruses that we provisionally refer to as the Quenyaviruses. Each of the five segments contains a single open reading frame, with most encoding proteins showing no detectable similarity to characterised viruses, and one sharing a small number of residues with the RNA-dependent RNA polymerases of single- and double-stranded RNA viruses. Using these sequences, we identify close relatives in approximately 20 arthropods, including insects, crustaceans, spiders, and a myriapod. Using a more conserved sequence from the putative polymerase, we further identify relatives in meta-transcriptomic datasets from gut, gill, and lung tissues of vertebrates, reflecting infections of vertebrates or of their associated parasites. Our data illustrate the utility of small RNAs to detect viruses with limited sequence conservation, and provide robust evidence for a new deeply divergent and phylogenetically distinct RNA virus lineage.
Recent climatic changes have the potential to severely alter river runoff, particularly in snow-dominated river basins. Effects of changing snow covers superimpose with changes in precipitation and anthropogenic modifications of the watershed and river network. In the attempt to identify and disentangle long-term effects of different mechanisms, we employ a set of analytical tools to extract long-term changes in river runoff at high resolution. We combine quantile sampling with moving average trend statistics and empirical mode decomposition and apply these tools to discharge data recorded along rivers with nival, pluvial and mixed flow regimes as well as temperature and precipitation data covering the time frame 1869-2016. With a focus on central Europe, we analyse the long-term impact of snow cover and precipitation changes along with their interaction with reservoir constructions.
Our results show that runoff seasonality of snow-dominated rivers decreases. Runoff increases in winter and spring, while discharge decreases in summer and at the beginning of autumn. We attribute this redistribution of annual flow mainly to reservoir constructions in the Alpine ridge. During the course of the last century, large fractions of the Alpine rivers were dammed to produce hydropower. In recent decades, runoff changes induced by reservoir constructions seem to overlap with changes in snow cover. We suggest that Alpine signals propagate downstream and affect runoff far outside the Alpine area in river segments with mixed flow regimes. Furthermore, our results hint at more (intense) rain-fall in recent decades. Detected increases in high discharge can be traced back to corresponding changes in precipitation.
We recently demonstrated that the sympathetic nervous system can be voluntarily activated following a training program consisting of cold exposure, breathing exercises, and meditation. This resulted in profound attenuation of the systemic inflammatory response elicited by lipopolysaccharide (LPS) administration. Herein, we assessed whether this training program affects the plasma metabolome and if these changes are linked to the immunomodulatory effects observed. A total of 224 metabolites were identified in plasma obtained from 24 healthy male volunteers at six timepoints, of which 98 were significantly altered following LPS administration. Effects of the training program were most prominent shortly after initiation of the acquired breathing exercises but prior to LPS administration, and point towards increased activation of the Cori cycle. Elevated concentrations of lactate and pyruvate in trained individuals correlated with enhanced levels of anti-inflammatory interleukin (IL)-10. In vitro validation experiments revealed that co-incubation with lactate and pyruvate enhances IL-10 production and attenuates the release of pro-inflammatory IL-1 beta and IL-6 by LPS-stimulated leukocytes. Our results demonstrate that practicing the breathing exercises acquired during the training program results in increased activity of the Cori cycle. Furthermore, this work uncovers an important role of lactate and pyruvate in the anti-inflammatory phenotype observed in trained subjects.
We recently demonstrated that the sympathetic nervous system can be voluntarily activated following a training program consisting of cold exposure, breathing exercises, and meditation. This resulted in profound attenuation of the systemic inflammatory response elicited by lipopolysaccharide (LPS) administration. Herein, we assessed whether this training program affects the plasma metabolome and if these changes are linked to the immunomodulatory effects observed. A total of 224 metabolites were identified in plasma obtained from 24 healthy male volunteers at six timepoints, of which 98 were significantly altered following LPS administration. Effects of the training program were most prominent shortly after initiation of the acquired breathing exercises but prior to LPS administration, and point towards increased activation of the Cori cycle. Elevated concentrations of lactate and pyruvate in trained individuals correlated with enhanced levels of anti-inflammatory interleukin (IL)-10. In vitro validation experiments revealed that co-incubation with lactate and pyruvate enhances IL-10 production and attenuates the release of pro-inflammatory IL-1 beta and IL-6 by LPS-stimulated leukocytes. Our results demonstrate that practicing the breathing exercises acquired during the training program results in increased activity of the Cori cycle. Furthermore, this work uncovers an important role of lactate and pyruvate in the anti-inflammatory phenotype observed in trained subjects.
Submerged sequences of marine terraces potentially provide crucial information of past sea-level positions. However, the distribution and characteristics of drowned marine terrace sequences are poorly known at a global scale. Using bathymetric data and novel mapping and modeling techniques, we studied a submerged sequence of marine terraces in the Bay of Biscay with the objective to identify the distribution and morphologies of submerged marine terraces and the timing and conditions that allowed their formation and preservation. To accomplish the objectives a high-resolution bathymetry (5 m) was analyzed using Geographic Information Systems and TerraceM(R). The successive submerged terraces were identified using a Surface Classification Model, which linearly combines the slope and the roughness of the surface to extract fossil sea-cliffs and fossil rocky shore platforms. For that purpose, contour and hillshaded maps were also analyzed. Then, shoreline angles, a geomorphic marker located at the intersection between the fossil sea-cliff and platform, were mapped analyzing swath profiles perpendicular to the isobaths. Most of the submerged strandlines are irregularly preserved throughout the continental shelf. In summary, 12 submerged terraces with their shoreline angles between approximately: -13 m (T1), -30 and -32 m (T2), -34 and 41 m (T3), -44 and -47 m (T4), -49 and 53 m (T5), -55 and 58 m (T6), -59 and 62 m (T7), -65 and 67 m (T8), -68 and 70 m (T9), -74 and -77 m (T10), -83 and -86 m (T11) and -89 and 92 m (T12). Nevertheless, the ones showing the best lateral continuity and preservation in the central part of the shelf are T3, T4, T5, T7, T8, and T10. The age of the terraces has been estimated using a landscape evolution model. To simulate the formation and preservation of submerged terraces three different scenarios: (i) 20-0 ka; (ii) 128-0 ka; and (iii) 128-20 ka, were compared. The best scenario for terrace generation was between 128 and 20 Ka, where T3, T5, and T7 could have been formed.
Submerged sequences of marine terraces potentially provide crucial information of past sea-level positions. However, the distribution and characteristics of drowned marine terrace sequences are poorly known at a global scale. Using bathymetric data and novel mapping and modeling techniques, we studied a submerged sequence of marine terraces in the Bay of Biscay with the objective to identify the distribution and morphologies of submerged marine terraces and the timing and conditions that allowed their formation and preservation. To accomplish the objectives a high-resolution bathymetry (5 m) was analyzed using Geographic Information Systems and TerraceM(R). The successive submerged terraces were identified using a Surface Classification Model, which linearly combines the slope and the roughness of the surface to extract fossil sea-cliffs and fossil rocky shore platforms. For that purpose, contour and hillshaded maps were also analyzed. Then, shoreline angles, a geomorphic marker located at the intersection between the fossil sea-cliff and platform, were mapped analyzing swath profiles perpendicular to the isobaths. Most of the submerged strandlines are irregularly preserved throughout the continental shelf. In summary, 12 submerged terraces with their shoreline angles between approximately: -13 m (T1), -30 and -32 m (T2), -34 and 41 m (T3), -44 and -47 m (T4), -49 and 53 m (T5), -55 and 58 m (T6), -59 and 62 m (T7), -65 and 67 m (T8), -68 and 70 m (T9), -74 and -77 m (T10), -83 and -86 m (T11) and -89 and 92 m (T12). Nevertheless, the ones showing the best lateral continuity and preservation in the central part of the shelf are T3, T4, T5, T7, T8, and T10. The age of the terraces has been estimated using a landscape evolution model. To simulate the formation and preservation of submerged terraces three different scenarios: (i) 20-0 ka; (ii) 128-0 ka; and (iii) 128-20 ka, were compared. The best scenario for terrace generation was between 128 and 20 Ka, where T3, T5, and T7 could have been formed.
The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between beta-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase beta-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and beta-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including beta-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-epsilon-cyclase (LCY epsilon), and beta-carotenoid hydroxylase (CHY beta) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and beta-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between beta-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase beta-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and beta-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including beta-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-epsilon-cyclase (LCY epsilon), and beta-carotenoid hydroxylase (CHY beta) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and beta-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
Under an ecological speciation scenario, the radiation of African weakly electric fish (genus Campylomormyrus) is caused by an adaptation to different food sources, associated with diversification of the electric organ discharge (EOD). This study experimentally investigates a phenotype-environment correlation to further support this scenario. Our behavioural experiments showed that three sympatric Campylomormyrus species with significantly divergent snout morphology differentially react to variation in substrate structure. While the short snout species (C. tamandua) exhibits preference to sandy substrate, the long snout species (C. rhynchophorus) significantly prefers a stone substrate for feeding. A third species with intermediate snout size (C. compressirostris) does not exhibit any substrate preference. This preference is matched with the observation that long-snouted specimens probe deeper into the stone substrate, presumably enabling them to reach prey more distant to the substrate surface. These findings suggest that the diverse feeding apparatus in the genus Campylomormyrus may have evolved in adaptation to specific microhabitats, i.e., substrate structures where these fish forage. Whether the parallel divergence in EOD is functionally related to this adaptation or solely serves as a prezygotic isolation mechanism remains to be elucidated.
Under an ecological speciation scenario, the radiation of African weakly electric fish (genus Campylomormyrus) is caused by an adaptation to different food sources, associated with diversification of the electric organ discharge (EOD). This study experimentally investigates a phenotype-environment correlation to further support this scenario. Our behavioural experiments showed that three sympatric Campylomormyrus species with significantly divergent snout morphology differentially react to variation in substrate structure. While the short snout species (C. tamandua) exhibits preference to sandy substrate, the long snout species (C. rhynchophorus) significantly prefers a stone substrate for feeding. A third species with intermediate snout size (C. compressirostris) does not exhibit any substrate preference. This preference is matched with the observation that long-snouted specimens probe deeper into the stone substrate, presumably enabling them to reach prey more distant to the substrate surface. These findings suggest that the diverse feeding apparatus in the genus Campylomormyrus may have evolved in adaptation to specific microhabitats, i.e., substrate structures where these fish forage. Whether the parallel divergence in EOD is functionally related to this adaptation or solely serves as a prezygotic isolation mechanism remains to be elucidated.
Photoluminescence spectroscopy is a widely applied characterization technique for semiconductor materials in general and halide perovskite solar cell materials in particular. It can give direct information on the recombination kinetics and processes as well as the internal electrochemical potential of free charge carriers in single semiconductor layers, layer stacks with transport layers, and complete solar cells. The correct evaluation and interpretation of photoluminescence requires the consideration of proper excitation conditions, calibration and application of the appropriate approximations to the rather complex theory, which includes radiative recombination, non-radiative recombination, interface recombination, charge transfer, and photon recycling. In this article, an overview is given of the theory and application to specific halide perovskite compositions, illustrating the variables that should be considered when applying photoluminescence analysis in these materials.
Photoluminescence spectroscopy is a widely applied characterization technique for semiconductor materials in general and halide perovskite solar cell materials in particular. It can give direct information on the recombination kinetics and processes as well as the internal electrochemical potential of free charge carriers in single semiconductor layers, layer stacks with transport layers, and complete solar cells. The correct evaluation and interpretation of photoluminescence requires the consideration of proper excitation conditions, calibration and application of the appropriate approximations to the rather complex theory, which includes radiative recombination, non-radiative recombination, interface recombination, charge transfer, and photon recycling. In this article, an overview is given of the theory and application to specific halide perovskite compositions, illustrating the variables that should be considered when applying photoluminescence analysis in these materials.
Transparent conductive materials based on indium oxide remain yet irreplaceable in various optoelectronic applications. Amorphous oxides appear especially attractive for technology as they are isotropic, demonstrate relatively high electron mobility and can be processed at low temperatures. Among them is indium zinc oxide (IZO) with a large zinc content that is crucial for keeping the amorphous state but redundant for the doping. In this work we investigated water-free and water containing IZO films obtained by radio frequency sputtering. The correlation between temperature driven changes of the chemical state, the optical and electrical properties as well as the progression of crystallization was in focus. Such characterization methods as: scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy, temperature dependent Hall-effect measurements and others were applied. Temperature dependent electrical properties of amorphous IZO and IZO:H2O films were found to evolve similarly. Based on our experience in In2O3:H2O (In2O3:H or IOH) we proposed an explanation for the changes observed. Water admixture was found to decrease crystallization temperature of IZO significantly from similar to 550 degrees C to similar to 280 degrees C. Herewith, the presence and concentration of water and/or hydroxyls was found to determine Zn distribution in the film. In particular, Zn enrichment was detected at the film's surface respective to the high water and/or hydroxyl amount. Raman spectra revealed a two-dimensional crystallization of w-ZnO which precedes regardless water presence an extensive In2O3 crystallization. An abrupt loss of electron mobility as a result of crystallization was attributed to the formation of ZnO interlayer on grain boundaries.
The origin of Asian monsoons
(2020)
The Cenozoic inception and development of the Asian monsoons remain unclear and have generated much debate, as several hypotheses regarding circulation patterns at work in Asia during the Eocene have been proposed in the few last decades. These include (a) the existence of modern-like monsoons since the early Eocene; (b) that of a weak South Asian monsoon (SAM) and little to no East Asian monsoon (EAM); or (c) a prevalence of the Intertropical Convergence Zone (ITCZ) migrations, also referred to as Indonesian-Australian monsoon (I-AM). As SAM and EAM are supposed to have been triggered or enhanced primarily by Asian palaeogeographic changes, their possible inception in the very dynamic Eocene palaeogeographic context remains an open question, both in the modelling and field-based communities. We investigate here Eocene Asian climate conditions using the IPSL-CM5A2 (Sepulchre et al., 2019) earth system model and revised palaeogeographies. Our Eocene climate simulation yields atmospheric circulation patterns in Asia substantially different from modern conditions. A large high-pressure area is simulated over the Tethys ocean, which generates intense low tropospheric winds blowing southward along the western flank of the proto-Himalayan-Tibetan plateau (HTP) system. This low-level wind system blocks, to latitudes lower than 10 degrees N, the migration of humid and warm air masses coming from the Indian Ocean. This strongly contrasts with the modern SAM, during which equatorial air masses reach a latitude of 20-25 degrees N over India and southeastern China. Another specific feature of our Eocene simulation is the widespread subsidence taking place over northern India in the midtroposphere (around 5000 m), preventing deep convective updraught that would transport water vapour up to the condensation level. Both processes lead to the onset of a broad arid region located over northern India and over the HTP. More humid regions of high seasonality in precipitation encircle this arid area, due to the prevalence of the Intertropical Convergence Zone (ITCZ) migrations (or Indonesian-Australian monsoon, I-AM) rather than monsoons. Although the existence of this central arid region may partly result from the specifics of our simulation (model dependence and palaeogeographic uncertainties) and has yet to be confirmed by proxy records, most of the observational evidence for Eocene monsoons are located in the highly seasonal transition zone between the arid area and the more humid surroundings. We thus suggest that a zonal arid climate prevailed over Asia before the initiation of monsoons that most likely occurred following Eocene palaeogeographic changes. Our results also show that precipitation seasonality should be used with caution to infer the presence of a monsoonal circulation and that the collection of new data in this arid area is of paramount importance to allow the debate to move forward.
The origin of Asian monsoons
(2020)
The Cenozoic inception and development of the Asian monsoons remain unclear and have generated much debate, as several hypotheses regarding circulation patterns at work in Asia during the Eocene have been proposed in the few last decades. These include (a) the existence of modern-like monsoons since the early Eocene; (b) that of a weak South Asian monsoon (SAM) and little to no East Asian monsoon (EAM); or (c) a prevalence of the Intertropical Convergence Zone (ITCZ) migrations, also referred to as Indonesian-Australian monsoon (I-AM). As SAM and EAM are supposed to have been triggered or enhanced primarily by Asian palaeogeographic changes, their possible inception in the very dynamic Eocene palaeogeographic context remains an open question, both in the modelling and field-based communities. We investigate here Eocene Asian climate conditions using the IPSL-CM5A2 (Sepulchre et al., 2019) earth system model and revised palaeogeographies. Our Eocene climate simulation yields atmospheric circulation patterns in Asia substantially different from modern conditions. A large high-pressure area is simulated over the Tethys ocean, which generates intense low tropospheric winds blowing southward along the western flank of the proto-Himalayan-Tibetan plateau (HTP) system. This low-level wind system blocks, to latitudes lower than 10 degrees N, the migration of humid and warm air masses coming from the Indian Ocean. This strongly contrasts with the modern SAM, during which equatorial air masses reach a latitude of 20-25 degrees N over India and southeastern China. Another specific feature of our Eocene simulation is the widespread subsidence taking place over northern India in the midtroposphere (around 5000 m), preventing deep convective updraught that would transport water vapour up to the condensation level. Both processes lead to the onset of a broad arid region located over northern India and over the HTP. More humid regions of high seasonality in precipitation encircle this arid area, due to the prevalence of the Intertropical Convergence Zone (ITCZ) migrations (or Indonesian-Australian monsoon, I-AM) rather than monsoons. Although the existence of this central arid region may partly result from the specifics of our simulation (model dependence and palaeogeographic uncertainties) and has yet to be confirmed by proxy records, most of the observational evidence for Eocene monsoons are located in the highly seasonal transition zone between the arid area and the more humid surroundings. We thus suggest that a zonal arid climate prevailed over Asia before the initiation of monsoons that most likely occurred following Eocene palaeogeographic changes. Our results also show that precipitation seasonality should be used with caution to infer the presence of a monsoonal circulation and that the collection of new data in this arid area is of paramount importance to allow the debate to move forward.
The emergence of carbapenemase-producing multi-drug resistant Enterobacteriaceae poses a dramatic, world-wide health risk. Limited treatment options and a lack of easy-to-use methods for the detection of infections with multi-drug resistant bacteria leave the health-care system with a fast-growing challenge. Aptamers are single stranded DNA or RNA molecules that bind to their targets with high affinity and specificity and can therefore serve as outstanding detection probes. However, an effective aptamer selection process is often hampered by non-specific binding. When selections are carried out against recombinant proteins, purification tags (e.g. polyhistidine) serve as attractive side targets, which may impede protein target binding. In this study, aptamer selection was carried out against N-terminally hexa-histidine tagged New Delhi metallo-ss-lactamase 1. After 14 selection rounds binding to polyhistidine was detected rather than to New Delhi metallo-ss-lactamase 1. Hence, the selection strategy was changed. As one aptamer candidate showed remarkable binding affinity to polyhistidine, it was used as a masking probe and selection was restarted from selection round 10. Finally, after three consecutive selection rounds, an aptamer with specific binding properties to New Delhi metallo-ss-lactamase 1 was identified. This aptamer may serve as a much-needed detection probe for New Delhi metallo-ss-lactamase 1 expressing Enterobacteriaceae.
In an ocean-continent subduction zone, the assessment of the lithospheric thermal state is essential to determine the controls of the deformation within the upper plate and the dip angle of the subducting lithosphere. In this study, we evaluate the degree of influence of both the configuration of the upper plate (i.e., thickness and composition of the rock units) and variations of the subduction angle on the lithospheric thermal field of the southern Central Andes (29 degrees-39 degrees S). Here, the subduction angle increases from subhorizontal (5 degrees) north of 33 degrees S to steep (similar to 30 degrees) in the south. We derived the 3D temperature and heat flow distribution of the lithosphere in the southern Central Andes considering conversion of S wave tomography to temperatures together with steady-state conductive thermal modeling. We found that the orogen is overall warmer than the forearc and the foreland and that the lithosphere of the northern part of the foreland appears colder than its southern counterpart. Sedimentary blanketing and the thickness of the radiogenic crust exert the main control on the shallow thermal field (<50km depth). Specific conditions are present where the oceanic slab is relatively shallow (<85 km depth) and the radiogenic crust is thin. This configuration results in relatively colder temperatures compared to regions where the radiogenic crust is thick and the slab is steep. At depths >50km, the temperatures of the overriding plate are mainly controlled by the mantle heat input and the subduction angle. The thermal field of the upper plate likely preserves the flat subduction angle and influences the spatial distribution of shortening.
Due to the fact that silicon (Si) increases the resistance of plants against diverse abiotic and biotic stresses, Si nowadays is categorized as beneficial substance for plants. However, humans directly influence Si cycling on a global scale. Intensified agriculture and corresponding harvest-related Si exports lead to Si losses in agricultural soils. This anthropogenic desilication might be a big challenge for modern agriculture. However, there is still only little knowledge about Si cycling in agricultural systems of the temperate zone, because most studies focus on rice and sugarcane production in (sub)tropical areas. Furthermore, many studies are performed for a short term only, and thus do not provide the opportunity to analyze slow changes in soil-plant systems (e.g., desilication) over long periods. We analyzed soil and plant samples from an ongoing long-term field experiment (established 1963) in the temperate zone (NE Germany) to evaluate the effects of different nitrogen-phosphoruspotassium (NPK) fertilization rates and crop straw recycling (i.e., straw incorporation) on anthropogenic desilication in the long term. Our results clearly show that crop straw recycling not only prevents anthropogenic desilication (about 43-60% of Si exports can be saved by crop straw recycling in the long term), but also replenishes plant available Si stocks of agricultural soil-plant systems. Furthermore, we found that a reduction of N fertilization rates of about 69% is possible without considerable biomass losses. This economy of the need for N fertilizers potentially can be combined with the benefits of crop straw recycling, i.e., enhancement of carbon sequestration via straw inputs and prevention of anthropogenic desilication of agricultural soil-plant systems. Thus crop straw recycling might have the potential to act as key management practice in sustainable, low fertilization agriculture in the temperate zone in the future.
Die Unternehmensgründung
(2022)
Gegenstand der anspruchsvollen Examensübungsklausur ist im ersten Teil ein aufsehenerregendes Urteil des OLG Hamm zu einer sog. cold-water-Challenge (NJOZ 2018, 1289). Eine große Herausforderung besteht zunächst in der Wahl eines übersichtlichen Aufbaus, der vollständig ist und zugleich Redundanzen vermeidet. Darüber hinaus eignet sich die Aufgabenstellung angesichts ihrer Stofffülle hervorragend zum Einüben der Schwerpunktsetzung. Im Detail bietet sich immer wieder die Möglichkeit, sich »nach oben abzusetzen«. Für eine gute Bewertung müssen nicht alle Einzelfragen diskutiert werden. Inhaltlich ist mit den Verkehrspflichten ein deliktsrechtlicher Klassiker zu bewerkstelligen, und eher unbekanntere Anspruchsgrundlagen aus dem Deliktsrecht wollen erkannt und subsumiert werden. Insoweit sind Auslegungs‑ und Argumentationsvermögen unter Beweis zu stellen. Sodann erfolgt ein Streifzug durch das Schadensrecht, wenn es um die Erstattung von Besuchskosten geht. Abschließend geht es im zweiten Teil um eine Entscheidung des BGH zu den umstrittenen Anforderungen an einen Kündigungsgrund nach dem Eintritt von Haushaltsangehörigen in den Mietvertrag infolge des Todes der Mieterpartei (BGHZ 217, 263 = NJW 2018, 2397), deren Kenntnis von Studierenden nicht erwartet werden kann. Die Grundzüge des Mietrechts müssen beherrscht und mit einem geschickten Argumentationsvermögen verbunden werden.
Das Verhältnis von Gemeinwohl und Gleichheit ist kein spannungsfreies. Soziale Gleichheit ist ein Grundwert liberal-demokratischer Gemeinwesen. Um diese Gleichheit zu bewahren, entwickelten sich im 20. Jahrhundert Konzeptionen von Gemeinwohl, die versuchten, das Gemeinwohl eher prozedural und pluralistisch zu verstehen. Eine zu spezifische, vorher festgelegte Definition des Gemeinwohls sei letzten Endes undemokratisch und ideologisch und somit der sozialen Gleichheit abträglich. In den letzten Jahren haben sich unter dem Oberbegriff des sozialen Egalitarismus jedoch auch die Vorstellungen der sozialen Gleichheit verändert, hin zu einem substanzielleren Verständnis, was die Frage aufwirft, ob prozedurale Gemeinwohlverständnisse ihrer Rolle als Wächter der Gleichheit immer noch gerecht werden können.
Background:
Corona contact tracing apps are a novel and promising measure to reduce the spread of COVID-19. They can help to balance the need to maintain normal life and economic activities as much as possible while still avoiding exponentially growing case numbers. However, a majority of citizens need to be willing to install such an app for it to be effective. Hence, knowledge about drivers for app uptake is crucial.
Objective:
This study aimed to add to our understanding of underlying psychological factors motivating app uptake. More specifically, we investigated the role of concern for one's own health and concern to unknowingly infect others.
Methods:
A two-wave survey with 346 German-speaking participants from Switzerland and Germany was conducted. We measured the uptake of two decentralized contact tracing apps officially launched by governments (Corona-Warn-App, Germany; SwissCovid, Switzerland), as well as concerns regarding COVID-19 and control variables.
Results:
Controlling for demographic variables and general attitudes toward the government and the pandemic, logistic regression analysis showed a significant effect of self-focused concerns (odds ratio [OR] 1.64, P=.002). Meanwhile, concern of unknowingly infecting others did not contribute significantly to the prediction of app uptake over and above concern for one's own health (OR 1.01, P=.92). Longitudinal analyses replicated this pattern and showed no support for the possibility that app uptake provokes changes in levels of concern. Testing for a curvilinear relationship, there was no evidence that "too much" concern leads to defensive reactions and reduces app uptake.
Conclusions:
As one of the first studies to assess the installation of already launched corona tracing apps, this study extends our knowledge of the motivational landscape of app uptake. Based on this, practical implications for communication strategies and app design are discussed.
Art. 102 AEUV, § 19 GWB und Verstoß gegen nicht-wettbewerbsrechtliche Vorschriften (hier: DSGVO)
(2023)
Keep on scrolling?
(2023)
Smartphones are an integral part of daily life for many people worldwide. However, concerns have been raised that long usage times and the fragmentation of daily life through smartphone usage are detrimental to well-being. This preregistered study assesses (1) whether differences in smartphone usage behaviors between individuals predict differences in a variety of well-being measures (between-person effects) and (2) whether differences in smartphone usage behaviors between situations predict whether an individual is feeling better or worse (within-person effects). In addition to total usage time, several indicators capturing the fragmentation of usage/nonusage time were developed. The study combines objectively measured smartphone usage with self-reports of well-being in surveys (N = 236) and an experience sampling period (N = 378, n = 5775 datapoints). To ensure the robustness of the results, we replicated our analyses in a second measurement period (surveys: N = 305; experience sampling: N = 534, n = 7287 datapoints) and considered the pattern of effects across different operational definitions and constructs. Results show that individuals who use their smartphone more report slightly lower well-being (between-person effect) but no evidence for within-person effects of total usage time emerged. With respect to fragmentation, we found no robust association with well-being.
„Fehlgeleitet“
(2020)
We investigate the initiation and early evolution of 12 solar eruptions, including six active-region hot channel and six quiescent filament eruptions, which were well observed by the Solar Dynamics Observatory, as well as by the Solar Terrestrial Relations Observatory for the latter. The sample includes one failed eruption and 11 coronal mass ejections, with velocities ranging from 493 to 2140 km s(-1). A detailed analysis of the eruption kinematics yields the following main results. (1) The early evolution of all events consists of a slow-rise phase followed by a main-acceleration phase, the height-time profiles of which differ markedly and can be best fit, respectively, by a linear and an exponential function. This indicates that different physical processes dominate in these phases, which is at variance with models that involve a single process. (2) The kinematic evolution of the eruptions tends to be synchronized with the flare light curve in both phases. The synchronization is often but not always close. A delayed onset of the impulsive flare phase is found in the majority of the filament eruptions (five out of six). This delay and its trend to be larger for slower eruptions favor ideal MHD instability models. (3) The average decay index at the onset heights of the main acceleration is close to the threshold of the torus instability for both groups of events (although, it is based on a tentative coronal field model for the hot channels), suggesting that this instability initiates and possibly drives the main acceleration.
We investigate the initiation and early evolution of 12 solar eruptions, including six active-region hot channel and six quiescent filament eruptions, which were well observed by the Solar Dynamics Observatory, as well as by the Solar Terrestrial Relations Observatory for the latter. The sample includes one failed eruption and 11 coronal mass ejections, with velocities ranging from 493 to 2140 km s(-1). A detailed analysis of the eruption kinematics yields the following main results. (1) The early evolution of all events consists of a slow-rise phase followed by a main-acceleration phase, the height-time profiles of which differ markedly and can be best fit, respectively, by a linear and an exponential function. This indicates that different physical processes dominate in these phases, which is at variance with models that involve a single process. (2) The kinematic evolution of the eruptions tends to be synchronized with the flare light curve in both phases. The synchronization is often but not always close. A delayed onset of the impulsive flare phase is found in the majority of the filament eruptions (five out of six). This delay and its trend to be larger for slower eruptions favor ideal MHD instability models. (3) The average decay index at the onset heights of the main acceleration is close to the threshold of the torus instability for both groups of events (although, it is based on a tentative coronal field model for the hot channels), suggesting that this instability initiates and possibly drives the main acceleration.
Fluorination of organic spacer impacts on the structural and optical response of 2D perovskites
(2020)
Low-dimensional hybrid perovskites have triggered significant research interest due to their intrinsically tunable optoelectronic properties and technologically relevant material stability. In particular, the role of the organic spacer on the inherent structural and optical features in two-dimensional (2D) perovskites is paramount for material optimization. To obtain a deeper understanding of the relationship between spacers and the corresponding 2D perovskite film properties, we explore the influence of the partial substitution of hydrogen atoms by fluorine in an alkylammonium organic cation, resulting in (Lc)(2)PbI4 and (Lf)(2)PbI4 2D perovskites, respectively. Consequently, optical analysis reveals a clear 0.2 eV blue-shift in the excitonic position at room temperature. This result can be mainly attributed to a band gap opening, with negligible effects on the exciton binding energy. According to Density Functional Theory (DFT) calculations, the band gap increases due to a larger distortion of the structure that decreases the atomic overlap of the wavefunctions and correspondingly bandwidth of the valence and conduction bands. In addition, fluorination impacts the structural rigidity of the 2D perovskite, resulting in a stable structure at room temperature and the absence of phase transitions at a low temperature, in contrast to the widely reported polymorphism in some non-fluorinated materials that exhibit such a phase transition. This indicates that a small perturbation in the material structure can strongly influence the overall structural stability and related phase transition of 2D perovskites, making them more robust to any phase change. This work provides key information on how the fluorine content in organic spacer influence the structural distortion of 2D perovskites and their optical properties which possess remarkable importance for future optoelectronic applications, for instance in the field of light-emitting devices or sensors.