A new generation of wavelength-tunable, fluorescent dyes, so-called DBD ([1,3]dioxolo[4,5-f][1,3]benzodioxole) dyes, were developed a few years ago, and they showed great potential as probes, for example, for fluorescence microscopy. However, their photophysics is not fully explored and leaves open questions regarding their large fluorescence Stokes shifts and sensitivity to solvent conditions of differently substituted DBD dyes. To improve the understanding of the influence of the substitution pattern of the DBD dyes on their respective photophysics, transient absorption spectroscopy (TAS) was used, that is, a pump-probe experiment on the femtosecond timescale. TAS allows measurements of excited states, ground state recovery, solvent relaxation, and fluorescence properties on time scales of up to several nanoseconds. Two different DBD dye samples were investigated: aryl- and ester-substituted DBD dyes. Experiments were carried out in solvents with different polarities using different excitation energies and at different viscosities. Based on the experimental data and theoretical calculations, we were able to determine the conformational changes of the molecule due to electronic excitation and were able to investigate solvent relaxation processes for both types of DBD dyes. By generalizing the theory for quadrupole-induced solvent relaxation developed by Togashi et al., we derived quadrupole moments of both molecules in the ground and excited state. Our data showed differences in the binding of polar solvent molecules to the dyes depending on the substituent on the DBD dye. In the case of water as the solvent, an additional efficient quenching process in the electronically excited state was revealed, which was indicated by the observation of solvated electrons in the TAS signals.

Plate tectonics is among the most important geological processes on Earth, but its emergence and evolution remain unclear. Here we extrapolate models of present-day plate tectonics to the past and propose that since about three billion years ago the rise of continents and the accumulation of sediments at continental edges and in trenches has provided lubrication for the stabilization of subduction and has been crucial in the development of plate tectonics on Earth. We conclude that the two largest surface erosion and subduction lubrication events occurred after the Palaeoproterozoic Huronian global glaciations (2.45 to 2.2 billion years ago), leading to the formation of the Columbia supercontinent, and after the Neoproterozoic ‘snowball’ Earth glaciations (0.75 to 0.63 billion years ago). The snowball Earth event followed the ‘boring billion’—a period of reduced plate tectonic activity about 1.75 to 0.75 billion years ago that was probably caused by a shortfall of sediments in trenches—and it kick-started the modern episode of active plate tectonics.

Mountains play a key role in the provision of nature’s contributions to people (NCP) worldwide that support societies’ quality of life. Simultaneously, mountains are threatened by multiple drivers of change. Due to the complex interlinkages between biodiversity, quality of life and drivers of change, research on NCP in mountains requires interdisciplinary approaches. In this study, we used the conceptual framework of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) and the notion of NCP to determine to what extent previous research on ecosystem services in mountains has explored the different components of the IPBES conceptual framework. We conducted a systematic review of articles on ecosystem services in mountains published up to 2016 using the Web of Science and Scopus databases. Descriptive statistical and network analyses were conducted to explore the level of research on the components of the IPBES framework and their interactions. Our results show that research has gradually become more interdisciplinary by studying higher number of NCP, dimensions of quality of life, and indirect drivers of change. Yet, research focusing on biodiversity, regulating NCP and direct drivers has decreased over time. Furthermore, despite the fact that research on NCP in mountains becoming more policy-oriented over time, mainly in relation to payments for ecosystem services, institutional responses remained underexplored in the reviewed studies. Finally, we discuss the relevant knowledge gaps that should be addressed in future research in order to contribute to IPBES.

Saturn’s main ring system is associated with a set of small moons that either are embedded within it or interact with the rings to alter their shape and composition. Five close flybys of the moons Pan, Daphnis, Atlas, Pandora, and Epimetheus were performed between December 2016 and April 2017 during the ring-grazing orbits of the Cassini mission. Data on the moons’ morphology, structure, particle environment, and composition were returned, along with images in the ultraviolet and thermal infrared. We find that the optical properties of the moons’ surfaces are determined by two competing processes: contamination by a red material formed in Saturn’s main ring system and accretion of bright icy particles or water vapor from volcanic plumes originating on the moon Enceladus.

Across a landscape, aquatic-terrestrial interfaces within and between ecosystems are hotspots of organic matter (OM) mineralization. These interfaces are characterized by sharp spatio-temporal changes in environmental conditions, which affect OM properties and thus control OM mineralization and other transformation processes. Consequently, the extent of OM movement at and across aquatic-terrestrial interfaces is crucial in determining OM turnover and carbon (C) cycling at the landscape scale. Here, we propose expanding current concepts in aquatic and terrestrial ecosystem sciences to comprehensively evaluate OM turnover at the landscape scale. We focus on three main concepts toward explaining OM turnover at the landscape scale: the landscape spatiotemporal context, OM turnover described by priming and ecological stoichiometry, and anthropogenic effects as a disruptor of natural OM transfer magnitudes and pathways. A conceptual framework is introduced that allows for discussing the disparities in spatial and temporal scales of OM transfer, changes in environmental conditions, ecosystem connectivity, and microbial-substrate interactions. The potential relevance of priming effects in both terrestrial and aquatic systems is addressed. For terrestrial systems, we hypothesize that the interplay between the influx of OM, its corresponding elemental composition, and the elemental demand of the microbial communities may alleviate spatial and metabolic thresholds. In comparison, substrate level OM dynamics may be substantially different in aquatic systems due to matrix effects that accentuate the role of abiotic conditions, substrate quality, and microbial community dynamics. We highlight the disproportionate impact anthropogenic activities can have on OM cycling across the landscape. This includes reversing natural OM flows through the landscape, disrupting ecosystem connectivity, and nutrient additions that cascade across the landscape. This knowledge is crucial for a better understanding of OM cycling in a landscape context, in particular since terrestrial and aquatic compartments may respond differently to the ongoing changes in climate, land use, and other anthropogenic interferences.

Der zweite Teil des bewährten Lehr- und Handbuchs Geschichte der deutschen Sprache enthält konzise und trotzdem leicht verständliche Darstellungen des Alt-, Mittel- und Frühneuhochdeutschen im Bereich der Phonologie, der Graphematik, der Morphologie und der Syntax. Dabei handelt es sich nicht nur um synchrone Beschreibungen des jeweiligen Sprachzustands, sondern auch um die Entwicklung des deutschen Sprachbaus auf allen strukturellen Ebenen. Die Verbindung von grammatischer Synchronie und struktureller Diachronie ist ein besonderes Markenzeichen dieses zweiten Teils der Schmidt'schen Sprachgeschichte.

The two hallmark features of Brownian motion are the linear growth < x2(t)> = 2Ddt of the mean squared displacement (MSD) with diffusion coefficient D in d spatial dimensions, and the Gaussian distribution of displacements. With the increasing complexity of the studied systems deviations from these two central properties have been unveiled over the years. Recently, a large variety of systems have been reported in which the MSD exhibits the linear growth in time of Brownian (Fickian) transport, however, the distribution of displacements is pronouncedly non-Gaussian (Brownian yet non-Gaussian, BNG). A similar behaviour is also observed for viscoelastic-type motion where an anomalous trend of the MSD, i.e., <x2(t)> ~ ta, is combined with a priori unexpected non-Gaussian distributions (anomalous yet non-Gaussian, ANG). This kind of behaviour observed in BNG and ANG diffusions has been related to the presence of heterogeneities in the systems and a common approach has been established to address it, that is, the random diffusivity approach. This dissertation explores extensively the field of random diffusivity models. Starting from a chronological description of all the main approaches used as an attempt of describing BNG and ANG diffusion, different mathematical methodologies are defined for the resolution and study of these models. The processes that are reported in this work can be classified in three subcategories, i) randomly-scaled Gaussian processes, ii) superstatistical models and iii) diffusing diffusivity models, all belonging to the more general class of random diffusivity models. Eventually, the study focuses more on BNG diffusion, which is by now well-established and relatively well-understood. Nevertheless, many examples are discussed for the description of ANG diffusion, in order to highlight the possible scenarios which are known so far for the study of this class of processes. The second part of the dissertation deals with the statistical analysis of random diffusivity processes. A general description based on the concept of moment-generating function is initially provided to obtain standard statistical properties of the models. Then, the discussion moves to the study of the power spectral analysis and the first passage statistics for some particular random diffusivity models. A comparison between the results coming from the random diffusivity approach and the ones for standard Brownian motion is discussed. In this way, a deeper physical understanding of the systems described by random diffusivity models is also outlined. To conclude, a discussion based on the possible origins of the heterogeneity is sketched, with the main goal of inferring which kind of systems can actually be described by the random diffusivity approach.

Tea aroma is one of the most important factors affecting the character and quality of tea. Here we describe the practical application of methyl jasmonate (MeJA) to improve the aroma quality of teas. The changes of selected metabolites during crucial tea processing steps, namely, withering, fixing and rolling, and fermentation, were analyzed. MeJA treatment of tea leaves (12, 24, 48, and 168 h) greatly promotes the aroma quality of green, oolong, and black tea products when comparing with untreated ones (0 h) and as confirmed by sensory evaluation. MeJA modulates the aroma profiles before, during, and after processing. Benzyl alcohol, benzaldehyde, 2-phenylethyl alcohol, phenylacetaldehyde, and trans-2-hexenal increased 1.07- to 3-fold in MeJA-treated fresh leaves and the first two maintained at a higher level in black tea and the last two in green tea. This correlates with a decrease in aromatic amino acids by more than twofold indicating a direct relation to tryptophan- and phenylalanine-derived volatiles. MeJA-treated oolong tea was characterized by a more pleasant aroma. Especially the terpenoids linalool and oxides, geraniol, and carvenol increased by more than twofold.

Objective The aim of this study was to examine the effects of two different sprint-training regimes on sprint and jump performances according to age in elite young male soccer players over the course of one soccer season. Methods Players were randomly assigned to two training groups. Group 1 performed systematic change-of-direction sprints (CODST, U19 [n = 9], U17 [n = 9], U15 [n = 10]) while group 2 conducted systematic linear sprints (LST, U19 [n = 9], U17 [n = 9], U15 [n = 9]). Training volumes were similar between groups (40 sprints per week x 30 weeks = 1200 sprints per season). Pre and post training, all players performed tests for the assessment of linear and slalom sprint speed (5-m and 10-m), countermovement jump, and maximal aerobic speed performance. Results For all physical fitness measures, the baseline-adjusted means data (ANCOVA) across the age groups showed no significant differences between LST and CODST at post (0.061 < p < 0.995; 0.0017 < d < 1.01). The analyses of baseline-adjusted means for all physical fitness measures for U15, U17, and U19 (LST vs. CODST) revealed no significant differences between LST and CODST for U15 (0.213 < p < 0.917; 0.001 < d < 0.087), U17 (0.132 < p < 0.976; 0.001 < d < 0.310), and U19 (0.300 < p < 0.999; 0.001 < d < 0.049) at post. Conclusions The results from this study showed that both, LST and CODST induced significant changes in the sprint, lower limbs power, and aerobic performances in young elite soccer players. Since no significant differences were observed between LST and CODST, the observed changes are most likely due to training and/or maturation. Therefore, more research is needed to elucidate whether CODST, LST or a combination of both is beneficial for youth soccer athletes’ performance development.

Mildred Harnack, geb. Fish, stammte ursprünglich aus Milwaukee, Wisconsin. Zusammen mit ihrem Ehemann Arvid Harnack zog sie nach Deutschland und lebte seit 1930 in Berlin. Hier lehrte die Literaturwissenschaftlerin an der Friedrich-Wilhelms-Universität (heute Humboldt-Universität) und am Berliner Abendgymnasium (heute Peter A. Silbermann-Schule). Bereits kurz nach der Machtübernahme von Adolf Hitler hatte sich um das Ehepaar Harnack ein Kreis von Freunden gebildet, der gegen die Herrschaft der Nationalsozialisten opponierte. Dazu zählten auch Karl Behrens und Bodo Schlösinger, die beide Schüler Mildred Harnacks am Berliner Abendgymnasium waren. Mildred Harnack konnte mit Hilfe ihrer Kontakte zur amerikanischen Botschaft ihren Schülern im nationalsozialistischen Deutschland ansonsten nicht zugängliche Informationen besorgen. Aufgrund von Funkkontakten des Freundeskreises zur Sowjetunion wurde die Gruppe von den Nationalsozialisten Rote Kapelle genannt – „rot“ bezog sich auf deren linke Haltung und mit „Kapelle“ wurden Funker assoziiert, die wie Pianisten in einer Kapelle spielen. Der Berliner Oppositionszirkel umfasste bis zu seiner Zerschlagung durch die Nationalsozialisten etwa 150 Personen verschiedenster Berufsgruppen, unterschiedlicher parteipolitischer Einstellungen und Konfessionen. Die Gruppe verfertigte oppositionelle Flugblätter und lieferte Informationen an die amerikanische Botschaft sowie an die Sowjetunion. Mildred Harnack wurde – wie viele ihrer Mitstreiterinnen und Mitstreiter – nach ihrer Verhaftung vom Reichskriegsgericht zum Tode verurteilt und am 16. Februar 1943 in Plötzensee guillotiniert. In diesem Band stellen Studierende der Universität Potsdam sowie Hörerinnen und Hörer der Peter A. Silbermann-Schule (Berlin) nach einem kurzen Überblick zum Widerstand gegen den Nationalsozialismus in Deutschland das Netzwerk der Roten Kapelle sowie die Biographien von Mildred Harnack und ihren Schülern Karl Behrens und Bodo Schlösinger vom Berliner Abendgymnasium eindrücklich vor.

Organizations continue to assemble and rely upon teams of remote workers as an essential element of their business strategy; however, knowledge processing is particular difficult in such isolated, largely digitally mediated settings. The great challenge for a knowledge-based organization lies not in how individuals should interact using technology but in how to achieve effective cooperation and knowledge exchange. Currently more attention has been paid to technology and the difficulties machines have processing natural language and less to studies of the human aspect—the influence of our own individual cognitive abilities and preferences on the processing of information when interacting online. This thesis draws on four scientific domains involved in the process of interpreting and processing massive, unstructured data—knowledge management, linguistics, cognitive science, and artificial intelligence—to build a model that offers a reliable way to address the ambiguous nature of language and improve workers’ digitally mediated interactions. Human communication can be discouragingly imprecise and is characterized by a strong linguistic ambiguity; this represents an enormous challenge for the computer analysis of natural language. In this thesis, I propose and develop a new data interpretation layer for the processing of natural language based on the human cognitive preferences of the conversants themselves. Such a semantic analysis merges information derived both from the content and from the associated social and individual contexts, as well as the social dynamics that emerge online. At the same time, assessment taxonomies are used to analyze online comportment at the individual and community level in order to successfully identify characteristics leading to greater effectiveness of communication. Measurement patterns for identifying effective methods of individual interaction with regard to individual cognitive and learning preferences are also evaluated; a novel Cyber-Cognitive Identity (CCI)—a perceptual profile of an individual’s cognitive and learning styles—is proposed. Accommodation of such cognitive preferences can greatly facilitate knowledge management in the geographically dispersed and collaborative digital environment. Use of the CCI is proposed for cognitively labeled Latent Dirichlet Allocation (CLLDA), a novel method for automatically labeling and clustering knowledge that does not rely solely on probabilistic methods, but rather on a fusion of machine learning algorithms and the cognitive identities of the associated individuals interacting in a digitally mediated environment. Advantages include: a greater perspicuity of dynamic and meaningful cognitive rules leading to greater tagging accuracy and a higher content portability at the sentence, document, and corpus level with respect to digital communication.

Glycosylphosphatidylinositols (GPIs) are highly complex glycolipids that serve as membrane anchors to a large variety of eukaryotic proteins. These are covalently attached to a group of peripheral proteins called GPI-anchored proteins (GPI-APs) through a post-translational modification in the endoplasmic reticulum. The GPI anchor is a unique structure composed of a glycan, with phospholipid tail at one end and a phosphoethanolamine linker at the other where the protein attaches. The glycan part of the GPI comprises a conserved pseudopentasaccharide core that could branch out to carry additional glycosyl or phosphoethanolamine units. GPI-APs are involved in a diverse range of cellular processes, few of which are signal transduction, protein trafficking, pathogenesis by protozoan parasites like the malaria- causing parasite Plasmodium falciparum. GPIs can also exist freely on the membrane surface without an attached protein such as those found in parasites like Toxoplasma gondii, the causative agent of Toxoplasmosis. These molecules are both structurally and functionally diverse, however, their structure-function relationship is still poorly understood. This is mainly because no clear picture exists regarding how the protein and the glycan arrange with respect to the lipid layer. Direct experimental evidence is rather scarce, due to which inconclusive pictures have emerged, especially regarding the orientation of GPIs and GPI-APs on membrane surfaces and the role of GPIs in membrane organization. It appears that computational modelling through molecular dynamics simulations would be a useful method to make progress. In this thesis, we attempt to explore characteristics of GPI anchors and GPI-APs embedded in lipid bilayers by constructing molecular models at two different resolutions – all-atom and coarse-grained. First, we show how to construct a modular molecular model of GPIs and GPI-anchored proteins that can be readily extended to a broad variety of systems, addressing the micro-heterogeneity of GPIs. We do so by creating a hybrid link to which GPIs of diverse branching and lipid tails of varying saturation with their optimized force fields, GLYCAM06 and Lipid14 respectively, can be attached. Using microsecond simulations, we demonstrate that GPI prefers to “flop-down” on the membrane, thereby, strongly interacting with the lipid heads, over standing upright like a “lollipop”. Secondly, we extend the model of the GPI core to carry out a systematic study of the structural aspects of GPIs carrying different side chains (parasitic and human GPI variants) inserted in lipid bilayers. Our results demonstrate the importance of the side branch residues as these are the most accessible, and thereby, recognizable epitopes. This finding qualitatively agrees with experimental observations that highlight the role of the side branches in immunogenicity of GPIs and the specificity thereof. The overall flop-down orientation of the GPIs with respect to the bilayer surface presents the side chain residues to face the solvent. Upon attaching the green fluorescent protein (GFP) to the GPI, it is seen to lie in close proximity to the bilayer, interacting both with the lipid heads and glycan part of the GPI. However the orientation of GFP is sensitive to the type of GPI it is attached to. Finally, we construct a coarse-grained model of the GPI and GPI-anchored GFP using a modified version of the MARTINI force-field, using which the timescale is enhanced by at least an order of magnitude compared to the atomistic system. This study provides a theoretical perspective on the conformational behavior of the GPI core and some of its branched variations in presence of lipid bilayers, as well as draws comparisons with experimental observations. Our modular atomistic model of GPI can be further employed to study GPIs of variable branching, and thereby, aid in designing future experiments especially in the area of vaccines and drug therapies. Our coarse-grained model can be used to study dynamic aspects of GPIs and GPI-APs w.r.t plasma membrane organization. Furthermore, the backmapping technique of converting coarse-grained trajectory back to the atomistic model would enable in-depth structural analysis with ample conformational sampling.

We report the design and characterization of photoactive yellow protein (PYP)-blue fluorescent protein (mTagBFP) fusion constructs that permit the direct assay of reconstitution and function of the PYP domain. These constructs allow for in vivo testing of co-expression systems for enzymatic production of the p-coumaric acid-based PYP chromophore, via the action of tyrosine ammonia lyase and p-coumaroyl-CoA ligase (pCL or 4CL). We find that different 4CL enzymes can function to reconstitute PYP, including 4CL from Arabidopsis thaliana that can produce similar to 100% holo-PYP protein under optimal conditions. mTagBFP fusion constructs additionally enable rapid analysis of effects of mutations on PYP photocycles. We use this mTagBFP fusion strategy to demonstrate in vivo reconstitution of several PYP-based optogenetic tools in Escherichia coli via a biosynthesized chromophore, an important step for the use of these optogenetic tools in vivo in diverse hosts.

Balanced expression of multiple genes is central for establishing new biosynthetic pathways or multiprotein cellular complexes. Methods for efficient combinatorial assembly of regulatory sequences (promoters) and protein coding sequences are therefore highly wanted. Here, we report a high-throughput cloning method, called COMPASS for COMbinatorial Pathway ASSembly, for the balanced expression of multiple genes in Saccharomyces cerevisiae. COMPASS employs orthogonal, plant-derived artificial transcription factors (ATFs) and homologous recombination-based cloning for the generation of thousands of individual DNA constructs in parallel. The method relies on a positive selection of correctly assembled pathway variants from both, in vivo and in vitro cloning procedures. To decrease the turnaround time in genomic engineering, COMPASS is equipped with multi-locus CRISPR/Cas9-mediated modification capacity. We demonstrate the application of COMPASS by generating cell libraries producing n-carotene and co-producing p-ionone and biosensor-responsive naringenin. COMPASS will have many applications in synthetic biology projects that require gene expression balancing.

We report on a combined theoretical and experimental study of core-excitation spectra of gas and liquid phase methanol as obtained with the use of X-ray absorption spectroscopy (XAS) and resonant inelastic X-ray scattering (RIXS). The electronic transitions are studied with computational methods that include strict and extended second-order algebraic diagrammatic construction [ADC(2) and ADC(2)-x], restricted active space second-order perturbation theory, and time-dependent density functional theory-providing a complete assignment of the near oxygen K-edge XAS. We show that multimode nuclear dynamics is of crucial importance for explaining the available experimental XAS and RIXS spectra. The multimode nuclear motion was considered in a recently developed "mixed representation" where dissociative states and highly excited vibrational modes are accurately treated with a time-dependent wave packet technique, while the remaining active vibrational modes are described using Franck-Condon amplitudes. Particular attention is paid to the polarization dependence of RIXS and the effects of the isotopic substitution on the RIXS profile in the case of dissociative core-excited states. Our approach predicts the splitting of the 2a RIXS peak to be due to an interplay between molecular and pseudo-atomic features arising in the course of transitions between dissociative core- and valence-excited states. The dynamical nature of the splitting of the 2a peak in RIXS of liquid methanol near pre-edge core excitation is shown. The theoretical results are in good agreement with our liquid phase measurements and gas phase experimental data available from the literature. (C) 2019 Author(s).

While extensive work has been dedicated to the measurement of the demagnetization time following an ultra-short laser pulse, experimental studies of its underlying microscopic mechanisms are still scarce. In transition metal ferromagnets, one of the main mechanism is the spin-flip of conduction electrons driven by electron-phonon scattering. Here, we present an original experimental method to monitor the electron-phonon mediated spin-flip scattering rate in nickel through the stringent atomic symmetry selection rules of x-ray emission spectroscopy. Increasing the phonon population leads to a waning of the 3d -> 2p(3/2) decay peak intensity, which reflects an increase of the angular momentum transfer scattering rate attributed to spin-flip. We find a spin relaxation time scale in the order of 50 fs in the 3d-band of nickel at room temperature, while consistantly, no such peak evolution is observed for the diamagnetic counterexample copper, using the same method.

Several studies have investigated the effects of music on both submaximal and maximal exercise performance at a constant work-rate. However, there is a lack of research that has examined the effects of music on the pacing strategy during self-paced exercise. The aim of this study was to examine the effects of preferred music on performance and pacing during a 6 min run test (6-MSPRT) in young male adults. Twenty healthy male participants volunteered for this study. They performed two randomly assigned trials (with or without music) of a 6-MSPRT three days apart. Mean running speed, the adopted pacing strategy, total distance covered (TDC), peak and mean heart rate (HRpeak, HRmean), blood lactate (3 min after the test), and rate of perceived exertion (RPE) were measured. Listening to preferred music during the 6-MSPRT resulted in significant TDC improvement (?10%; p = 0.016; effect size (ES) = 0.80). A significantly faster mean running speed was observed when listening to music compared with no music. The improvement of TDC in the present study is explained by a significant overall increase in speed (main effect for conditions) during the music trial. Music failed to modify pacing patterns as suggested by the similar reversed “J-shaped” profile during the two conditions. Blood-lactate concentrations were significantly reduced by 9% (p = 0.006, ES = 1.09) after the 6-MSPRT with music compared to those in the control condition. No statistically significant differences were found between the test conditions for HRpeak, HRmean, and RPE. Therefore, listening to preferred music can have positive effects on exercise performance during the 6-MSPRT, such as greater TDC, faster running speeds, and reduced blood lactate levels but has no effect on the pacing strategy.

Due to continuously intensifying human usage of the marine environment worldwide ranging cetaceans face an increasing number of threats. Besides whaling, overfishing and by-catch, new technical developments increase the water and noise pollution, which can negatively affect marine species. Cetaceans are especially prone to these influences, being at the top of the food chain and therefore accumulating toxins and contaminants. Furthermore, they are extremely noise sensitive due to their highly developed hearing sense and echolocation ability. As a result, several cetacean species were brought to extinction during the last century or are now classified as critically endangered. This work focuses on two odontocetes. It applies and compares different molecular methods for inference of population status and adaptation, with implications for conservation. The worldwide distributed sperm whale (Physeter macrocephalus) shows a matrilineal population structure with predominant male dispersal. A recently stranded group of male sperm whales provided a unique opportunity to investigate male grouping for the first time. Based on the mitochondrial control region, I was able to infer that male bachelor groups comprise multiple matrilines, hence derive from different social groups, and that they represent the genetic variability of the entire North Atlantic. The harbor porpoise (Phocoena phocoena) occurs only in the northern hemisphere. By being small and occurring mostly in coastal habitats it is especially prone to human disturbance. Since some subspecies and subpopulations are critically endangered, it is important to generate and provide genetic markers with high resolution to facilitate population assignment and subsequent protection measurements. Here, I provide the first harbour porpoise whole genome, in high quality and including a draft annotation. Using it for mapping ddRAD seq data, I identify genome wide SNPs and, together with a fragment of the mitochondrial control region, inferred the population structure of its North Atlantic distribution range. The Belt Sea harbors a distinct subpopulation oppose to the North Atlantic, with a transition zone in the Kattegat. Within the North Atlantic I could detect subtle genetic differentiation between western (Canada-Iceland) and eastern (North Sea) regions, with support for a German North Sea breading ground around the Isle of Sylt. Further, I was able to detect six outlier loci which show isolation by distance across the investigated sampling areas. In employing different markers, I could show that single maker systems as well as genome wide data can unravel new information about population affinities of odontocetes. Genome wide data can facilitate investigation of adaptations and evolutionary history of the species and its populations. Moreover, they facilitate population genetic investigations, providing a high resolution, and hence allowing for detection of subtle population structuring especially important for highly mobile cetaceans.

A common feature in Answer Set Programming is the use of a second negation, stronger than default negation and sometimes called explicit, strong or classical negation. This explicit negation is normally used in front of atoms, rather than allowing its use as a regular operator. In this paper we consider the arbitrary combination of explicit negation with nested expressions, as those defined by Lifschitz, Tang and Turner. We extend the concept of reduct for this new syntax and then prove that it can be captured by an extension of Equilibrium Logic with this second negation. We study some properties of this variant and compare to the already known combination of Equilibrium Logic with Nelson's strong negation.

Inflammatory diseases of the gastrointestinal tract are emerging as a global problem with increased evidence and prevalence in numerous countries. A dysregulated sphingolipid metabolism occurs in patients with ulcerative colitis and is discussed to contribute to its pathogenesis. In the present study, we determined the impact of acid sphingomyelinase (Asm), which catalyzes the hydrolysis of sphingomyelin to ceramide, on the course of Citrobacter (C.) rodentium-driven colitis. C. rodentium is an enteric pathogen and induces colonic inflammation very similar to the pathology in patients with ulcerative colitis. We found that mice with Asm deficiency or Asm inhibition were strongly susceptible to C. rodentium infection. These mice showed increased levels of C. rodentium in the feces and were prone to bacterial spreading to the systemic organs. In addition, mice lacking Asm activity showed an uncontrolled inflammatory T(h)1 and T(h)17 response, which was accompanied by a stronger colonic pathology compared to infected wild type mice. These findings identified Asm as an essential regulator of mucosal immunity to the enteric pathogen C. rodentium.

The tremendous success of metal-halide perovskites, especially in the field of photovoltaics, has triggered a substantial number of studies in understanding their optoelectronic properties. However, consensus regarding the electronic properties of these perovskites is lacking due to a huge scatter in the reported key parameters, such as work function (Φ) and valence band maximum (VBM) values. Here, we demonstrate that the surface photovoltage (SPV) is a key phenomenon occurring at the perovskite surfaces that feature a non-negligible density of surface states, which is more the rule than an exception for most materials under study. With ultraviolet photoelectron spectroscopy (UPS) and Kelvin probe, we evidence that even minute UV photon fluxes (500 times lower than that used in typical UPS experiments) are sufficient to induce SPV and shift the perovskite Φ and VBM by several 100 meV compared to dark. By combining UV and visible light, we establish flat band conditions (i.e., compensate the surface-state-induced surface band bending) at the surface of four important perovskites, and find that all are p-type in the bulk, despite a pronounced n-type surface character in the dark. The present findings highlight that SPV effects must be considered in all surface studies to fully understand perovskites’ photophysical properties.

Marine snow aggregates represent heterogeneous agglomerates of dead and living organic matter. Composition is decisive for their sinking rates, and thereby for carbon flux to the deep sea. For oligotrophic oceans, information on aggregate composition is particularly sparse. To address this, the taxonomic composition of aggregates collected from the subtropical and oligotrophic Sargasso Sea (Atlantic Ocean) was characterized by 16S and 18S rRNA gene sequencing. Taxonomy assignment was aided by a collection of the contemporary plankton community consisting of 75 morphologically and genetically identified plankton specimens. The diverse rRNA gene reads of marine snow aggregates, not considering Trichodesmium puffs, were dominated by copepods (52%), cnidarians (21%), radiolarians (11%), and alveolates (8%), with sporadic contributions by cyanobacteria, suggesting a different aggregate composition than in eutrophic regions. Composition linked significantly with sampling location but not to any measured environmental parameters or plankton biomass composition. Nevertheless, indicator and network analyses identified key roles of a few rare taxa. This points to complex regulation of aggregate composition, conceivably affected by the environment and plankton characteristics. The extent to which this has implications for particle densities, and consequently for sinking rates and carbon sequestration in oligotrophic waters, needs further interrogation.

Charge extraction in organic solar cells (OSCs) is commonly believed to be limited by bimolecular recombination of photogenerated charges. However, the fill factor of OSCs is usually almost entirely governed by recombination processes that scale with the first order of the light intensity. This linear loss was often interpreted to be a consequence of geminate or trap-assisted recombination. Numerical simulations show that this linear dependence is a direct consequence of the large amount of excess dark charge near the contact. The first-order losses increase with decreasing mobility of minority carriers, and we discuss the impact of several material and device parameters on this loss mechanism. This work highlights that OSCs are especially vulnerable to injected charges as a result of their poor charge transport properties. This implies that dark charges need to be better accounted for when interpreting electro-optical measurements and charge collection based on simple figures of merit.

High Harmonic Generation (HHG) is a nonlinear optical process that provides a tunable source for high-energy photons and ultrashort laser pulses. Recent experiments demonstrated that HHG spectroscopy may also be used as an analytical tool to discriminate between randomly oriented configurational isomers of polyatomic organic molecules, namely, between the cis- and trans-forms of 1,2-dichloroethene (DCE) [M. C. H. Wong et al., Phys. Rev. A 84, 051403 (2011)]. Here, we suggest as an economic and at the same time a reasonably accurate method to compute HHG spectra for polyatomic species, Time-Dependent Configuration Interaction Singles (TD-CIS) theory in combination with extended atomic orbital bases and different models to account for ionization losses. The HHG spectra are computed for aligned and unaligned cis- and trans-DCE. For the unaligned case, a coherent averaging over possible rotational orientations is introduced. Furthermore, using TD-CIS, possible differences between the HHG spectra of cis- and trans-DCE are studied. For aligned molecules, spectral differences between cis and trans emerge, which can be related to their different point group symmetries. For unaligned, randomly oriented molecules, we also find distinct HHG spectra in partial agreement with experiment. In addition to HHG response in the frequency space, we compute time-frequency HHG spectra to gain insight into which harmonics are emitted at which time. Further differences between the two isomers emerge, suggesting time-frequency HHG as another tool to discriminate configurational isomers.

The physical and hydrological properties of peat from seven peatlands in northern Maputaland (South Africa) were investigated and related to the degradation processes of peatlands in different hydrogeomorphic settings. The selected peatlands are representative of typical hydrogeomorphic settings and different stages of human modification from natural to severely degraded. Nineteen transects (141 soil corings in total) were examined in order to describe peat properties typical of the distinct hydrogeomorphic settings. We studied degree of decomposition, organic matter content, bulk density, water retention, saturated hydraulic conductivity and hydrophobicity of the peats. From these properties we derived pore size distribution, unsaturated hydraulic conductivity and maximum capillary rise. We found that, after drainage, degradation advances faster in peatlands containing wood peat than in peatlands containing radicell peat. Eucalyptus plantations in catchment areas are especially threatening to peatlands in seeps, interdune depressions and unchannelled valley bottoms. All peatlands and their recharge areas require wise management, especially valley-bottom peatlands with swamp forest vegetation. Blocking drainage ditches is indispensable as a first step towards achieving the restoration of drained peatland areas, and further measures may be necessary to enhance the distribution of water. The sensitive swamp forest ecosystems should be given conservation priority.

Almost half of the political life has been experienced under the state of emergency and state of siege policies in the Turkish Republic. In spite of such a striking number and continuity in the deployment of legal emergency powers, there are just a few legal and political studies examining the reasons for such permanency in governing practices. To fill this gap, this paper aims to discuss one of the most important sources of the ‘permanent’ political crisis in the country: the historical evolution of legal emergency power. In order to highlight how these policies have intensified the highly fragile citizenship regime by weakening the separation of power, repressing the use of political rights and increasing the discretionary power of both the executive and judiciary authorities, the paper sheds light on the emergence and production of a specific form of legality based on the idea of emergency and the principle of executive prerogative. In that context, it aims to provide a genealogical explanation of the evolution of the exceptional form of the nation-state, which is based on the way political society, representation, and legitimacy have been instituted and accompanying failure of the ruling classes in building hegemony in the country.

In this study, a low-cost unmanned aerial vehicle was used to obtain multi-spectral high-resolution imagery (1.4 cmpx(-1)) from2 microcatchments (3.3 ha) with burned Mediterranean shrubland and pine forests. This imagery was used to calculate the blue normalized differential vegetation index and to generate digital elevation models for estimating the sediment connectivity index. Both indices enabled an integrated approach for deciphering how hydrological and sediment connectivity interact with vegetation as well as soil conservation structures. The application of spatial analysis improves our understanding of the feedback between biological and geomorphological processes. Local spatial data analysis established a significant link between local geomorphological and biological factors, enabling a precise identification of homogeneous areas at micro-catchment scale and the minimal size of vegetation units reacting to geomorphology as natural groups at plot-scale where management strategies and efforts should be applied. Establishing this local relationship between sediment connectivity and vegetation patterns through new and interdisciplinary methodologies represents a new strategy for the assessment of ecosystem dynamics and management.

The present study aims to identify the optimal body-size/shape and maturity characteristics associated with superior fitness test performances having controlled for body-size, sex, and chronological-age differences. The sample consisted of 597 Tunisian children (396 boys and 201 girls) aged 8 to 15 years. Three sprint speeds recorded at 10, 20 and 30 m; two vertical and two horizontal jump tests; a change-of-direction and a handgrip-strength tests, were assessed during physical-education classes. Allometric modelling was used to identify the benefit of being an early or late maturer. Findings showed that being tall and light is the ideal shape to be successful at most physical fitness tests, but the height-to-weight “shape” ratio seems to be test-dependent. Having controlled for body-size/shape, sex, and chronological age, the model identified maturity-offset as an additional predictor. Boys who go earlier/younger through peak-height-velocity (PHV) outperform those who go at a later/older age. However, most of the girls’ physical-fitness tests peaked at the age at PHV and decline thereafter. Girls whose age at PHV was near the middle of the age range would appear to have an advantage compared to early or late maturers. These findings have important implications for talent scouts and coaches wishing to recruit children into their sports/athletic clubs.

Worin besteht gutes Philosophieren? Und weshalb ist gerade John Stuart Mill ein außergewöhnlich guter Philosoph? Joachim Toenges-Hinn verbindet in diesem Band die metaphilosophische Suche danach, was gute Philosophie ausmacht, mit einer historischen Betrachtung des Philosophen John Stuart Mill. Dabei fungiert Mill zugleich als Urheber von und Verkörperung des Strebens nach zwei philosophischen Tugenden, die Toenges-Hinn aus Mills philosophischem Werk ableitet und anschließend systematisch verteidigt. Diese als „Bentham-Ideal“ und „Coleridge-Ideal“ bezeichneten Tugenden stehen dabei ebenso im Fokus seiner Untersuchung wie die Bedeutung von Lebensexperimenten für philosophische Biografien.

Water can adsorb molecularly or dissociatively onto different sites of metal oxide surfaces. These adsorption sites can be disentangled using surface-sensitive vibrational spectroscopy. Here, we model Vibrational Sum Frequency (VSF) spectra for various forms of dissociated, deuterated water on a reconstructed, Al-terminated α-Al2O3(0001) surface at submonolayer coverages (the so-called 1-2, 1-4, and 1-4′ modes). Using an efficient scheme based on velocity-velocity autocorrelation functions, we go beyond previous normal mode analyses by including anharmonicity, mode coupling, and thermal surface motion in the framework of ab initio molecular dynamics. In this way, we calculate vibrational density of states curves, infrared, and VSF spectra. Comparing computed VSF spectra with measured ones, we find that relative frequencies of resonances are in quite good agreement and linewidths are reasonably well represented, while VSF intensities coincide not well. We argue that intensities are sensitively affected by local interactions and thermal fluctuations, even at such low coverage, while absolute peak positions strongly depend on the choice of the electronic structure method and on the appropriate inclusion of anharmonicity.

Sekundäre Pflanzenstoffe und ihre gesundheitsfördernden Eigenschaften sind in den letzten zwei Jahrzehnten vielfach ernährungsphysiologisch untersucht und spezifische positive Effek-te im humanen Organismus zum Teil sehr genau beschrieben worden. Zu den Carotinoiden zählend ist der sekundäre Pflanzenstoff Lutein insbesondere in der Prävention von ophthal-mologischen Erkrankungen in den Mittelpunkt der Forschung gerückt. Das ausschließlich von Pflanzen und einigen Algen synthetisierte Xanthophyll wird über die pflanzliche Nahrung insbesondere grünes Blattgemüse in den humanen Organismus aufgenommen. Dort akkumu-liert es bevorzugt im Makulapigment der Retina des menschlichen Auges und ist bedeutend im Prozess der Aufrechterhaltung der Funktionsfähigkeit der Photorezeptorzellen. Im Laufe des Alterns kann die Abnahme der Dichte des Makulapigments und der Abbau von Lutein beobachtet werden. Die dadurch eintretende Destabilisierung der Photorezeptorzellen im Zu-sammenhang mit einer veränderten Stoffwechsellage im alternden Organismus kann zur Aus-prägung der altersbedingten Makuladegeneration (AMD) führen. Die pathologische Sympto-matik der Augenerkrankung reicht vom Verlust der Sehschärfe bis hin zum irreversiblen Er-blinden. Da therapeutische Mittel ausschließlich ein Fortschreiten verhindern, bestehen hier Forschungsansätze präventive Maßnahmen zu finden. Die Supplementierung von luteinhalti-gen Präparaten bietet dabei einen Ansatzpunkt. Auf dem Markt finden sich bereits Nahrungs-ergänzungsmittel (NEM) mit Lutein in verschiedenen Applikationen. Limitierend ist dabei die Stabilität und Bioverfügbarkeit von Lutein, welches teilweise kostenintensiv und mit unbe-kannter Reinheit zu erwerben ist. Aus diesem Grund wäre die Verwendung von Luteinestern als die pflanzliche Speicherform des Luteins im Rahmen eines NEMs vorteilhaft. Neben ihrer natürlichen, höheren Stabilität sind Luteinester nachhaltig und kostengünstig einsetzbar. In dieser Arbeit wurden physikochemische und ernährungsphysiologisch relevante Aspekte in dem Produktentwicklungsprozess eines NEMs mit Luteinestern in einer kolloidalen Formulie-rung untersucht. Die bisher einzigartige Anwendung von Luteinestern in einem Mundspray sollte die Aufnahme des Wirkstoffes insbesondere für ältere Menschen erleichtern und verbes-sern. Unter Beachtung der Ergebnisse und der ernährungsphysiologischen Bewertung sollten u.a. Empfehlungen für die Rezepturzusammensetzungen einer Miniemulsion (Emulsion mit Partikelgrößen <1,0 µm) gegeben werden. Eine Einschätzung der Bioverfügbarkeit der Lutei-nester aus den entwickelten, kolloidalen Formulierungen konnte anhand von Studien zur Re-sorption- und Absorptionsverfügbarkeit in vitro ermöglicht werden. In physikalischen Untersuchungen wurden zunächst Basisbestandteile für die Formulierungen präzisiert. In ersten wirkstofffreien Musteremulsionen konnten ausgewählte Öle als Trägerpha-se sowie Emulgatoren und Löslichkeitsvermittler (Peptisatoren) hinsichtlich ihrer Eignung zur Bereitstellung einer Miniemulsion physikalisch geprüft werden. Die beste Stabilität und opti-male Eigenschaften einer Miniemulsion zeigten sich bei der Verwendung von MCT-Öl (engl. medium chain triglyceride) bzw. Rapsöl in der Trägerphase sowie des Emulga-tors Tween® 80 (Tween 80) allein oder in Kombination mit dem Molkenproteinhydrolysat Biozate® 1 (Biozate 1). Aus den physikalischen Untersuchungen der Musteremulsionen gingen die Präemulsionen als Prototypen hervor. Diese enthielten den Wirkstoff Lutein in verschiedenen Formen. So wur-den Präemulsionen mit Lutein, mit Luteinestern sowie mit Lutein und Luteinestern konzipiert, welche den Emulgator Tween 80 oder die Kombination mit Biozate 1 enthielten. Bei der Her-stellung der Präemulsionen führte die Anwendung der Emulgiertechniken Ultraschall mit an-schließender Hochdruckhomogenisation zu den gewünschten Miniemulsionen. Beide einge-setzten Emulgatoren boten optimale Stabilisierungseffekte. Anschließend erfolgte die phy-sikochemische Charakterisierung der Wirkstoffe. Insbesondere Luteinester aus Oleoresin er-wiesen sich hier als stabil gegenüber verschiedenen Lagerungsbedingungen. Ebenso konnte bei einer kurzzeitigen Behandlung der Wirkstoffe unter spezifischen mechanischen, thermischen, sauren und basischen Bedingungen eine Stabilität von Lutein und Luteinestern gezeigt wer-den. Die Zugabe von Biozate 1 bot dabei nur für Lutein einen zusätzlichen Schutz. Bei länge-rer physikochemischer Behandlung unterlagen die in den Miniemulsionen eingebrachten Wirk-stoffe moderaten Abbauvorgängen. Markant war deren Sensitivität gegenüber dem basischen Milieu. Im Rahmen der Rezepturentwicklung des NEMs war hier die Empfehlung, eine Mi-niemulsion mit einem leicht saurem pH-Milieu zum Schutz des Wirkstoffes durch kontrollierte Zugabe weiterer Inhaltstoffe zu gestalten. Im weiteren Entwicklungsprozess des NEMs wurden Fertigrezepturen mit dem Wirkstoff Luteinester aufgestellt. Die alleinige Anwendung des Emulgators Biozate 1 zeigte sich dabei als ungeeignet. Die weiterhin zur Verfügung stehenden Fertigrezepturen enthielten in der Öl-phase neben dem Wirkstoff das MCT-ÖL oder Rapsöl sowie -Tocopherol zur Stabilisierung. Die Wasserphase bestand aus dem Emulgator Tween 80 oder einer Kombination aus Tween 80 und Biozate 1. Zusatzstoffe waren zudem als mikrobiologischer Schutz Ascorbin-säure und Kaliumsorbat sowie für sensorische Effekte Xylitol und Orangenaroma. Die An-ordnung der Basisrezeptur und das angewendete Emulgierverfahren lieferten stabile Mi-niemulsionen. Weiterhin zeigten langfristige Lagerungsversuche mit den Fertigrezepturen bei 4°C, dass eine Aufrechterhaltung der geforderten Luteinestermenge im Produkt gewähr-leistet war. Analoge Untersuchungen an einem luteinhaltigen, marktgängigen Präparat bestä-tigten dagegen eine bereits bei kurzfristiger Lagerung auftretende Instabilität von Lutein. Abschließend wurde durch Resorptions- und Absorptionsstudien in vitro mit den Präemulsio-nen und Fertigrezepturen die Bioverfügbarkeit von Luteinestern geprüft. Nach Behandlung in einem etablierten in vitro Verdaumodell konnte eine geringfügige Resorptionsverfügbarkeit der Luteinester definiert werden. Limitiert war eine Micellarisierung des Wirkstoffes aus den konzipierten Formulierungen zu beobachten. Eine enzymatische Spaltung der Luteinester zu freiem Lutein wurde nur begrenzt festgestellt. Spezifität und Aktivität von entsprechenden hydrolytischen Lipasen sind als äußerst gering gegenüber Luteinestern zu bewerten. In sich anschließenden Zellkulturversuchen mit der Zelllinie Caco-2 wurden keine zytotoxischen Ef-fekte durch die relevanten Inhaltsstoffe in den Präemulsionen gezeigt. Dagegen konnten eine Sensibilität gegenüber den Fertigrezepturen beobachtet werden. Diese sollte im Zusammen-hang mit Irritationen der Schleimhäute des Magen-Darm-Traktes bedacht werden. Eine weni-ger komplexe Rezeptur könnte die beobachteten Einschränkungen möglicherweise minimieren. Abschließende Absorptionsstudien zeigten, dass grundsätzlich eine geringfügige Aufnahme von vorrangig Lutein, aber auch Luteinmonoestern in den Enterocyten aus Miniemulsionen erfolgen kann. Dabei hatte weder Tween 80 noch Biozate 1 einen förderlichen Einfluss auf die Absorptionsrate von Lutein oder Luteinestern. Die Metabolisierung der Wirkstoffe durch vorherigen in vitro-Verdau steigerte die zelluläre Aufnahme von Wirkstoffen aus Formulie-rungen mit Lutein und Luteinestern gleichermaßen. Die beobachtete Aufnahme von Lutein und Luteinmonoestern in den Enterocyten scheint über passive Diffusion zu erfolgen, wobei auch der aktive Transport nicht ausgeschlossen werden kann. Dagegen können Luteindiester aufgrund ihrer Molekülgröße nicht über den Weg der Micellarisierung und einfachen Diffusi-on in die Enterocyten gelangen. Ihre Aufnahme in die Dünndarmepithelzellen bedarf einer vorherigen hydrolytischen Spaltung durch spezifische Lipasen. Dieser Schritt limitiert wiede-rum die effektive Aufnahme der Luteinester in die Zellen bzw. stellt eine Einschränkung in ihrer Bioverfügbarkeit im Vergleich zu freiem Lutein dar. Zusammenfassend konnte für die physikochemisch stabilen Luteinester eine geringe Biover-fügbarkeit aus kolloidalen Formulierungen gezeigt werden. Dennoch ist die Verwendung als Wirkstoffquelle für den sekundären Pflanzenstoff Lutein in einem NEM zu empfehlen. Im Zusammenhang mit der Aufnahme von luteinreichen, pflanzlichen Lebensmitteln kann trotz der zu erwartenden geringen Bioverfügbarkeit der Luteinester aus dem NEM ein Beitrag zur Verbesserung des Luteinstatus erreicht werden. Entsprechende Publikationen zeigten eindeu-tige Korrelationen zwischen der Aufnahme von luteinesterhaltigen Präparaten und einem An-stieg der Luteinkonzentration im Serum bzw. der Makulapigmentdichte in vivo. Die geringfü-gig bessere Bioverfügbarkeit von freiem Lutein steht im kritischen Zusammenhang mit seiner Instabilität und Kostenintensität. Bilanzierend wurde im Rahmen dieser Arbeit das marktgän-gige Produkt Vita Culus® konzipiert. Im Ausblick sollten humane Interventionsstudien mit dem NEM die abschließende Bewertung der Bioverfügbarkeit von Luteinestern aus dem Prä-parat möglich machen.

The East Asian monsoons characterize the modern-day Asian climate, yet their geological history and driving mechanisms remain controversial. The southeasterly summer monsoon provides moisture, whereas the northwesterly winter monsoon sweeps up dust from the arid Asian interior to form the Chinese Loess Plateau. The onset of this loess accumulation, and therefore of the monsoons, was thought to be 8 million years ago (Ma). However, in recent years these loess records have been extended further back in time to the Eocene (56-34 Ma), a period characterized by significant changes in both the regional geography and global climate. Yet the extent to which these reconfigurations drive atmospheric circulation and whether the loess-like deposits are monsoonal remains debated. In this thesis, I study the terrestrial deposits of the Xining Basin previously identified as Eocene loess, to derive the paleoenvironmental evolution of the region and identify the geological processes that have shaped the Asian climate. I review dust deposits in the geological record and conclude that these are commonly represented by a mix of both windblown and water-laid sediments, in contrast to the pure windblown material known as loess. Yet by using a combination of quartz surface morphologies, provenance characteristics and distinguishing grain-size distributions, windblown dust can be identified and quantified in a variety of settings. This has important implications for tracking aridification and dust-fluxes throughout the geological record. Past reversals of Earth’s magnetic field are recorded in the deposits of the Xining Basin and I use these together with a dated volcanic ash layer to accurately constrain the age to the Eocene period. A combination of pollen assemblages, low dust abundances and other geochemical data indicates that the early Eocene was relatively humid suggesting an intensified summer monsoon due to the warmer greenhouse climate at this time. A subsequent shift from predominantly freshwater to salt lakes reflects a long-term aridification trend possibly driven by global cooling and the continuous uplift of the Tibetan Plateau. Superimposed on this aridification are wetter intervals reflected in more abundant lake deposits which correlate with highstands of the inland proto-Paratethys Sea. This sea covered the Eurasian continent and thereby provided additional moisture to the winter-time westerlies during the middle to late Eocene. The long-term aridification culminated in an abrupt shift at 40 Ma reflected by the onset of windblown dust, an increase in steppe-desert pollen, the occurrence of high-latitude orbital cycles and northwesterly winds identified in deflated salt deposits. Together, these indicate the onset of a Siberian high atmospheric pressure system driving the East Asian winter monsoon as well as dust storms and was triggered by a major sea retreat from the Asian interior. These results therefore show that the proto-Paratethys Sea, though less well recognized than the Tibetan Plateau and global climate, has been a major driver in setting up the modern-day climate in Asia.

Das Wort der "Genialität der Gerechtigkeit" stammt von Nietzsche selbst, der bekannte, dass er diese Form der Genialität mindestens ebenso hoch schätze wie alle übrigen. Gerade in den letzten Jahren seit Erscheinen der ersten Auflage ist die Problematik der Gerechtigkeit bei Nietzsche so stark in den Vordergrund gerückt wie lange nicht. Die Neuauflage unternimmt vor diesem Hintergrund den Versuch einer Neubewertung.

This study aimed to compare the training load of a professional under-19 soccer team (U-19) to that of an elite adult team (EAT), from the same club, during the in-season period. Thirty-nine healthy soccer players were involved (EAT [n = 20]; U-19 [n = 19]) in the study which spanned four weeks. Training load (TL) was monitored as external TL, using a global positioning system (GPS), and internal TL, using a rating of perceived exertion (RPE). TL data were recorded after each training session. During soccer matches, players’ RPEs were recorded. The internal TL was quantified daily by means of the session rating of perceived exertion (session-RPE) using Borg’s 0–10 scale. For GPS data, the selected running speed intensities (over 0.5 s time intervals) were 12–15.9 km/h; 16–19.9 km/h; 20–24.9 km/h; >25 km/h (sprint). Distances covered between 16 and 19.9 km/h, > 20 km/h and >25 km/h were significantly higher in U-19 compared to EAT over the course of the study (p = 0.023, d = 0.243, small; p = 0.016, d = 0.298, small; and p = 0.001, d = 0.564, small, respectively). EAT players performed significantly fewer sprints per week compared to U-19 players (p = 0.002, d = 0.526, small). RPE was significantly higher in U-19 compared to EAT (p = 0.001, d = 0.188, trivial). The external and internal measures of TL were significantly higher in the U-19 group compared to the EAT soccer players. In conclusion, the results obtained show that the training load is greater in U19 compared to EAT.

Isolated extreme habitats are ideally suited to investigate pivotal ecological processes such as niche use, local adaptation and dispersal. Extremophilic animals living in isolated habitats face the problem that dispersal is limited through the absence of suitable dispersal corridors, which in turn facilitates local adaptation. We used five rotifer isolates from extremely acidic mining lakes with a pH of below 3 as model organisms to test whether these isolates are acidotolerant or acidophilic, whether they survive and reproduce at their niche edges (here pH 2 and circum-neutral pH) and whether local adaptation has evolved. To evaluate potential dispersal limitation, we tested whether animals and their parthenogenetic eggs survive and remain reproductive or viable at unfavourable pH-conditions. All five isolates were acidophilic with a pH-optimum in the range of 4-6, which is well above the pH (< 3) of their lakes of origin. At unfavourable high pH, in four out of the five isolates parthenogenetic females produced a high number of non-viable eggs. Females and eggs produced at favourable pH (4) remained vital at an otherwise unfavourable pH of 7, indicating that for dispersal no acidic dispersal corridors are necessary. Common garden experiments revealed no clear evidence for local adaptation in any of the five isolates. Despite their acidophilic nature, all five isolates can potentially disperse via circum-neutral water bodies as long as their residence time is short, suggesting a broader dispersal niche than their realized niche. Local adaptation might have been hampered by the low population sizes of the rotifers in their isolated habitat and the short time span the mining lakes have existed.

Transverse dispersion, or tracer spreading orthogonal to the mean flow direction, which is relevant e.g, for quantifying bio-degradation of contaminant plumes or mixing of reactive solutes, has been studied in the literature less than the longitudinal one. Inferring transverse dispersion coefficients from field experiments is a difficult and error-prone task, requiring a spatial resolution of solute plumes which is not easily achievable in applications. In absence of field data, it is a questionable common practice to set transverse dispersivities as a fraction of the longitudinal one, with the ratio 1/10 being the most prevalent. We collected estimates of field-scale transverse dispersivities from existing publications and explored possible scale relationships as guidance criteria for applications. Our investigation showed that a large number of estimates available in the literature are of low reliability and should be discarded from further analysis. The remaining reliable estimates are formation-specific, span three orders of magnitude and do not show any clear scale-dependence on the plume traveled distance. The ratios with the longitudinal dispersivity are also site specific and vary widely. The reliability of transverse dispersivities depends significantly on the type of field experiment and method of data analysis. In applications where transverse dispersion plays a significant role, inference of transverse dispersivities should be part of site characterization with the transverse dispersivity estimated as an independent parameter rather than related heuristically to longitudinal dispersivity.

For effective disaster risk management and adaptation planning, a good understanding of current and projected flood risk is required. Recent advances in quantifying flood risk at the regional and global scale have largely neglected critical infrastructure, or addressed this important sector with insufficient detail. Here, we present the first European-wide assessment of current and future flood risk to railway tracks for different global warming scenarios using an infrastructure-specific damage model. We find that the present risk, measured as expected annual damage, to railway networks in Europe is approx. (sic)581 million per year, with the highest risk relative to the length of the network in North Macedonia, Croatia, Norway, Portugal, and Germany. Based on an ensemble of climate projections for RCP8.5, we show that current risk to railway networks is projected to increase by 255% under a 1.5 degrees C, by 281% under a 2 degrees C, and by 310% under a 3 degrees C warming scenario. The largest increases in risk under a 3 degrees C scenario are projected for Slovakia, Austria, Slovenia, and Belgium. Our advances in the projection of flood risk to railway infrastructure are important given their criticality, and because losses to public infrastructure are usually not insured or even uninsurable in the private market. To cover the risk increase due to climate change, European member states would need to increase expenditure in transport by (sic)1.22 billion annually under a 3 degrees C warming scenario without further adaptation. Limiting global warming to the 1.5 degrees C goal of the Paris Agreement would result in avoided losses of (sic)317 million annually.

Context. X-ray radiation from accreting compact objects is an important part of stellar feedback. The metal-poor galaxy ESO 338-4 has experienced vigorous starburst during the last <40 Myr and contains some of the most massive super star clusters in the nearby Universe. Given its starburst age and its star-formation rate, ESO 338-4 is one of the most efficient nearby manufactures of neutron stars and black holes, hence providing an excellent laboratory for feedback studies. Aims. We aim to use X-ray observations with the largest modern X-ray telescopes XMM-Newton and Chandra to unveil the most luminous accreting neutron stars and black holes in ESO 338-4. Methods. We compared X-ray images and spectra with integral field spectroscopic observations in the optical to constrain the nature of strong X-ray emitters. Results. X-ray observations uncover three ultraluminous X-ray sources (ULXs) in ESO 338-4. The brightest among them, ESO 338 X-1, has X-ray luminosity in excess of 10(40) erg s(-1). We speculate that ESO 338-4 X-1 is powered by accretion on an intermediate-mass (greater than or similar to 300 M-circle dot)black hole. We show that X-ray radiation from ULXs and hot superbubbles strongly contributes to He II ionization and general stellar feedback in this template starburst galaxy.

The [4 + 2] cycloadditions of singlet oxygen to 9,10-diphenylanthracene (1) and the meta and para isomers of 9,10-dipyridylanthracene (2m/p) and 9,10-methoxyphenylanthracene (3m/p) have been studied by density functional calculations in the gas phase at the UB3LYP/6-31G* level and for the first time in solvents at the conductor-like polarizable continuum model (CPCM) UM062X/6-31G* level. The differences in calculated transition state (TS) energies derived from this method are in line with experimentally observed reactivity orders in solution. For the gas-phase reaction, the first TS of the stepwise pathway (TS1) has biradical character, and its energy lies below the energy of the TS of the concerted path (TSconc). In contrast, in the solvent acetonitrile, TS1 resembles a zwitterion and lies significantly higher than the TSconc. Thus, a concerted mechanism applies in solvents, and the energy gap between the TS of the two processes decreases with decreasing polarity. A change from a pyridyl against a methoxyphenyl substituent in the para position causes a maximal reduction of the activation barrier by approximately 1.7 kcal/mol, resulting in a fivefold increased reactivity.

Agricultural land-use practices have intensified over the last decades, leading to population declines of various farmland species, including the European hare (Lepus europaeus). In many European countries, arable fields dominate agricultural landscapes. Compared to pastures, arable land is highly variable, resulting in a large spatial variation of food and cover for wildlife over the course of the year, which potentially affects habitat selection by hares. Here, we investigated within-home-range habitat selection by hares in arable areas in Denmark and Germany to identify habitat requirements for their conservation. We hypothesized that hare habitat selection would depend on local habitat structure, that is, vegetation height, but also on agricultural field size, vegetation type, and proximity to field edges. Active hares generally selected for short vegetation (1-25 cm) and avoided higher vegetation and bare ground, especially when fields were comparatively larger. Vegetation >50 cm potentially restricts hares from entering parts of their home range and does not provide good forage, the latter also being the case on bare ground. The vegetation type was important for habitat selection by inactive hares, with fabaceae, fallow, and maize being selected for, potentially providing both cover and forage. Our results indicate that patches of shorter vegetation could improve the forage quality and habitat accessibility for hares, especially in areas with large monocultures. Thus, policymakers should aim to increase areas with short vegetation throughout the year. Further, permanent set-asides, like fallow and wildflower areas, would provide year-round cover for inactive hares. Finally, the reduction in field sizes would increase the density of field margins, and farming different crop types within small areas could improve the habitat for hares and other farmland species.

Thermokarst results from the thawing of ice-rich permafrost and alters the biogeochemical cycling in the Arctic by reworking soil material and redistributing soil organic carbon (SOC) and total nitrogen (TN) along uplands, hillslopes, and lowlands. Understanding the impact of this redistribution is key to better estimating the storage of SOC in permafrost terrains. However, there are insufficient studies quantifying long-term impacts of thaw processes on the distribution of SOC and TN along hillslopes. We address this issue by providing estimates of SOC and TN stocks along the hillslopes of three valleys located on Herschel Island (Yukon, Canada), and by discussing the impact of hillslope thermokarst on the variability of SOC and TN stocks. We found that the average SOC and TN 0-100 cm stocks in the valleys were 26.4 +/- 8.9 kg C m(-2) and 2.1 +/- 0.6 kg N m(-2). We highlight the strong variability in the soils physical and geochemical properties within hillslope positions. High SOC stocks were found at the summits, essentially due to burial of organic matter by cryoturbation, and at the toeslopes due to impeded drainage which favored peat formation and SOC accumulation. The average carbon-to-nitrogen ratio in the valleys was 12.9, ranging from 9.7 to 18.9, and was significantly higher at the summits compared to the backslopes and footslopes (p < 0.05), suggesting a degradation of SOC downhill. Carbon and nitrogen contents and stocks were significantly lower on 16% of the sites that were previously affected by hillslope thermokarst (p < 0.05). Our results showed that lateral redistribution of SOC and TN due to hillslope thermokarst has a strong impact on the SOC storage in ice-rich permafrost terrains.

The current study tests the hypothesis that shy children’s reduced word learning is partly due to an effect of shyness on attention during object labeling. A sample of 20- and 26-month-old children (N = 32) took part in a looking-while-listening task in which they saw sets of familiar and novel objects while hearing familiar or novel labels. Overall, children increased attention to familiar objects when hearing their labels, and they divided their attention equally between the target and competitors when hearing novel labels. Critically, shyness reduced attention to the target object regardless of whether the heard label was novel or familiar. When children’s retention of the novel word–object mappings was tested after a delay, it was found that children who showed increased attention to novel objects during labeling showed better retention. Taken together, these findings suggest that shyer children perform less well than their less shy peers on measures of word learning because their attention to the target object is dampened. Thus, this work presents evidence that shyness modulates the low-level processes of visual attention that unfold during word learning.

Decoupling of optical properties appears challenging, but vital to get better insight of the relationship between light and fruit attributes. In this study, nine solid phantoms capturing the ranges of absorption (μa) and reduced scattering (μs’) coefficients in fruit were analysed non-destructively using laser-induced backscattering imaging (LLBI) at 1060 nm. Data analysis of LLBI was carried out on the diffuse reflectance, attenuation profile obtained by means of Farrell’s diffusion theory either calculating μa [cm−1] and μs’ [cm−1] in one fitting step or fitting only one optical variable and providing the other one from a destructive analysis. The nondestructive approach was approved when calculating one unknown coefficient non-destructively, while no ability of the method was found to analysis both, μa and μs’, non-destructively. Setting μs’ according to destructive photon density wave (PDW) spectroscopy and fitting μa resulted in root mean square error (rmse) of 18.7% in comparison to fitting μs’ resulting in rmse of 2.6%, pointing to decreased measuring uncertainty, when the highly variable μa was known. The approach was tested on European pear, utilizing destructive PDW spectroscopy for setting one variable, while LLBI was applied for calculating the remaining coefficient. Results indicated that the optical properties of pear obtained from PDW spectroscopy as well as LLBI changed concurrently in correspondence to water content mainly. A destructive batch-wise analysis of μs’ and online analysis of μa may be considered in future developments for improved fruit sorting results, when considering fruit with high variability of μs’.

Many process model analysis techniques rely on the accurate analysis of the natural language contents captured in the models’ activity labels. Since these labels are typically short and diverse in terms of their grammatical style, standard natural language processing tools are not suitable to analyze them. While a dedicated technique for the analysis of process model activity labels was proposed in the past, it suffers from considerable limitations. First of all, its performance varies greatly among data sets with different characteristics and it cannot handle uncommon grammatical styles. What is more, adapting the technique requires in-depth domain knowledge. We use this paper to propose a machine learning-based technique for activity label analysis that overcomes the issues associated with this rule-based state of the art. Our technique conceptualizes activity label analysis as a tagging task based on a Hidden Markov Model. By doing so, the analysis of activity labels no longer requires the manual specification of rules. An evaluation using a collection of 15,000 activity labels demonstrates that our machine learning-based technique outperforms the state of the art in all aspects.

Previous studies have shown that multilingual speakers are influenced by their native (L1) and non-native (L2) grammars when learning a new language. But, so far, these studies have mostly used untimed metalinguistic tasks. Here we examine whether multilinguals’ prior grammars also affect their sensitivity to morphosyntactic constraints during processing. We use speeded judgment and self-paced reading tasks to examine the comprehension of German possessive pronouns. To investigate whether native and non-native grammars differentially affect participants’ performance, we compare two groups of non-native German speakers with inverse L1–L2 distributions: a group with L1 Spanish – L2 English, and a group with L1 English – L2 Spanish. We show that the reading profiles of both groups are modulated by their L1 grammar, with L2 proficiency selectively affecting participants’ judgment accuracy but not their reading times. We propose that reading comprehension is mainly influenced by multilinguals’ native grammar, but that knowledge of an L2 grammar can further increase sensitivity to morphosyntactic violations in an additional language.

A wide variety of processes controls the time of occurrence, duration, extent, and severity of river floods. Classifying flood events by their causative processes may assist in enhancing the accuracy of local and regional flood frequency estimates and support the detection and interpretation of any changes in flood occurrence and magnitudes. This paper provides a critical review of existing causative classifications of instrumental and preinstrumental series of flood events, discusses their validity and applications, and identifies opportunities for moving toward more comprehensive approaches. So far no unified definition of causative mechanisms of flood events exists. Existing frameworks for classification of instrumental and preinstrumental series of flood events adopt different perspectives: hydroclimatic (large-scale circulation patterns and atmospheric state at the time of the event), hydrological (catchment scale precipitation patterns and antecedent catchment state), and hydrograph-based (indirectly considering generating mechanisms through their effects on hydrograph characteristics). All of these approaches intend to capture the flood generating mechanisms and are useful for characterizing the flood processes at various spatial and temporal scales. However, uncertainty analyses with respect to indicators, classification methods, and data to assess the robustness of the classification are rarely performed which limits the transferability across different geographic regions. It is argued that more rigorous testing is needed. There are opportunities for extending classification methods to include indicators of space-time dynamics of rainfall, antecedent wetness, and routing effects, which will make the classification schemes even more useful for understanding and estimating floods. This article is categorized under: Science of Water > Water Extremes Science of Water > Hydrological Processes Science of Water > Methods