Refine
Has Fulltext
- no (1) (remove)
Year of publication
- 2015 (1) (remove)
Document Type
- Article (1)
Language
- English (1)
Is part of the Bibliography
- yes (1)
Keywords
- fluorescent reporter (1) (remove)
Institute
- Institut für Biochemie und Biologie (1) (remove)
Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used in other areas of protein engineering. Here we report an approach for screening light-switchable DNA-binding proteins that relies on light-dependent repression of the transcription of a fluorescent reporter. We demonstrate that the method can be used to recover a known light-switchable DNA-binding protein from a random library.