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The Russian-Siberian journey, carried out with Alexander von Humboldt and Gustav Rose in 1829 coincided with a decisive phase in Christian Gottfried Ehrenberg’s scientific career. His field diary and the drawings he made on the trip document his intensified focus on the study of microorganisms. The numerous observations on plant and animal geography show how Ehrenberg tested a combination of micro- and macro-perspectives that would shape his research on infusoria in the following decades: In addition to the task of classification at the microscope, Ehrenberg carried out worldwide comparative research on the distribution of microorganisms.