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Keywords
- Lumineszenz (2)
- OTDR (2)
- molecular oxygen (2)
- optical sensing (2)
- triangular-[4] phenylene (2)
- Absorption (1)
- Biophotonik (1)
- Energietransfer (1)
- Fluoreszenz-Resonanz-Energie-Transfer (1)
- Förster Resonanz Energie Transfer (1)
Institute
A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.