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Reeve, Holly A. ; Nicholson, Jake ; Altaf, Farieha ; Lonsdale, Thomas H. ; Preissler, Janina ; Lauterbach, Lars ; Lenz, Oliver ; Leimkühler, Silke ; Hollmann, Frank ; Paul, Caroline E. ; Vincent, Kylie A.
We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H-2.
Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide.
The biocatalyst system retained >50% activity after 7 h.