Refine
Language
- English (13)
Is part of the Bibliography
- yes (13) (remove)
Keywords
- Antibody generation (2)
- Assay systems (2)
- cells (2)
- efficient (2)
- endothelial cells (2)
- gene delivery (2)
- immunogenicity (2)
- lipoplexes (2)
- mediated delivery (2)
- pathways (2)
In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.