The role of myoglobin degradation in the formation of zinc protoporphyrin IX in the longissimus lumborum of pork
- Investigations on the post mortal formation of fluorescent zinc protoporphyrin (ZnPP) IX in pork meat are currently in focus of meat science research. The role of myoglobin degradation in this context appears to be one of the most diversely discussed issues. To address this question meat-extracts of longissimus lumborum (LL) muscle (0.8 mg/mL) were incubated at 30 degrees C for up to 72 h and investigated by HPSEC-UV-fluorescence, SDS-PAGE and MALDI-TOF-MS. Between 0 and 72 h of incubation the fluorescence intensity (lambda(ex)./(em). = 420/590 nm) of the meat-extracts rose significantly (p < 0.001) from 10.9 +/- 0.8 to 34.8 +/- 0.3 (rel. units) while the staining intensity of the SDS-PAGE of myoglobin non-significantly (p > 0.4) changed from 6.2 +/- 0.5 x 105 to 5.0 +/- 0.3 x 105 (rel. units). The results indicate that ZnPP is formed by a Fe(II)-Zn(II)-substitution in myoglobin heme where an accompanying myoglobin degradation is not necessarily obligatory. (C) 2017 Elsevier Ltd. All rights reserved.
Verfasserangaben: | Amin Ghadiri Khozroughi, Elisabeth Jander, Michael SchirrmannGND, Harshadrai Manilal RawelORCiDGND, Lothar W. Kroh, Oliver Schlueter |
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DOI: | https://doi.org/10.1016/j.lwt.2017.06.047 |
ISSN: | 0023-6438 |
ISSN: | 1096-1127 |
Titel des übergeordneten Werks (Englisch): | LWT - food science and technology : an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) |
Verlag: | Elsevier |
Verlagsort: | Amsterdam |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2017 |
Erscheinungsjahr: | 2017 |
Datum der Freischaltung: | 20.04.2020 |
Freies Schlagwort / Tag: | Fluorescence; Meat; Post mortem chemistry; Proteolysis |
Band: | 85 |
Seitenanzahl: | 6 |
Erste Seite: | 22 |
Letzte Seite: | 27 |
Fördernde Institution: | Avicenna foundation; Federal Ministry of Education and Research of Germany |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
Peer Review: | Referiert |