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Binding and degradation of juvenile hormone III by haemolymph proteins of the Colorado potato beetle: a re-examination

  • The haemolymph of the adult Colorado potato beetle, Lepinotarsa decemlineata Say, contains a high molecular weight (MW > 200,000) JH-III specific binding protein. The Kd value of the protein for racemic JH-III is 1.3 ± 0.2 × 10−7 M. It has a lower affinity for racemic JH-I and it does not bind JH-III-diol or JH-III-acid. The binding protein does discriminate between the enantiomers of synthetic, racemic JH-III as was determined by stereochemical anaysis of the bound and the free JH-III. Incubation of racemic JH-III with crude haemolymph results in preferential formation of (10S)-JH-III-acid, the unnatural configuration. The JH-esterase present in L. decemlineata haemolymph is not enantioselective. It is concluded that the most important function of the binding protein is that of a specific carrier, protecting the natural hormone against degradation by esterases. The carrier does not protect JH-I as efficiently as the lower homologue.

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Author:C. A. D. de Kort, Martin G. Peter, A. B. Koopmanschap
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 068)
Document Type:Postprint
Year of Completion:1983
Publishing Institution:Universität Potsdam
Release Date:2008/08/27
Tag:JH-III-specific carrier protein; Juvenile hormone; Leptinotarsa decemlineata; enantioselectivity
Source:Insect Biochemistry. - 13 (1983), 5, pp. 481 - 487. ISSN 0020-1790
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Extern / Extern
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoKeine Nutzungslizenz vergeben - es gilt das deutsche Urheberrecht
Notes extern:
first published in:
Insect Biochemistry. - 13 (1983), 5, pp. 481 - 487
ISSN 0020-1790
DOI 10.1016/0020-1790(83)90005-7