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Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica)
- The James Ross archipelago houses numerous lakes and ponds. In this region, a vast diatom and cyanobacterial variety has been reported; however, the prokaryotic diversity in microbial mats from these lakes remains poorly explored. Here, a high-throughput sequencing of 16S rRNA gene in microbial mats from Lake Bart-Roja in James Ross Island and lakes Pan Negro and North Pan Negro located in Vega Island was performed. Combined with mineralogical and environmental characteristics, we analyzed the diversity and structure of the microbial communities. Sequences assigned to Archaea were extremely low, while Bacteria domain prevailed with the abundance of Proteobacteria (mostly Betaproteobacteriales) followed by Bacteroidetes, Verrucomicrobia, Firmicutes, and Cyanobacteria. Local environmental conditions, such as conductivity and Eh, provided differential microbial assemblages that might have implications in the oligotrophic status of the lakes. Consequently, a clear segregation at the family level was observed. In this sense, theThe James Ross archipelago houses numerous lakes and ponds. In this region, a vast diatom and cyanobacterial variety has been reported; however, the prokaryotic diversity in microbial mats from these lakes remains poorly explored. Here, a high-throughput sequencing of 16S rRNA gene in microbial mats from Lake Bart-Roja in James Ross Island and lakes Pan Negro and North Pan Negro located in Vega Island was performed. Combined with mineralogical and environmental characteristics, we analyzed the diversity and structure of the microbial communities. Sequences assigned to Archaea were extremely low, while Bacteria domain prevailed with the abundance of Proteobacteria (mostly Betaproteobacteriales) followed by Bacteroidetes, Verrucomicrobia, Firmicutes, and Cyanobacteria. Local environmental conditions, such as conductivity and Eh, provided differential microbial assemblages that might have implications in the oligotrophic status of the lakes. Consequently, a clear segregation at the family level was observed. In this sense, the assigned diversity was related to taxa recognized as denitrifiers and sulfur oxidizers. Particularly, in Lake Pan Negro sulfur-reducing and methanogenic representatives were also found and positively correlate with alkalinity and water depth. Moreover, Deinococcus-Thermus was observed in Lake Bart-Roja, while Melainabacteria (Cyanobacteria)-poorly reported in Antarctic mats-was detected in Lake Pan Negro. Epsilonbacteraeota was exclusively found in this lake, suggesting new potential phylotypes. This study contributes to the understanding of the diversity, composition, and structure of Antarctic benthic microbial ecosystems and provides highly valuable information, which can be used as a proxy to evaluate environmental changes affecting Antarctic microbiota.…
Author details: | Guillermo Cesar Fernández, Karina LecomteORCiD, Paula VignoniORCiDGND, Eliana Marcela Soto-RuedaORCiD, Silvia H. Coria, Juan Manuel LirioORCiD, Estela Cecilia MlewskiORCiD |
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DOI: | https://doi.org/10.1007/s00300-021-02997-z |
ISSN: | 0722-4060 |
ISSN: | 1432-2056 |
Title of parent work (English): | Polar biology : current research and development in science and technology |
Publisher: | Springer |
Place of publishing: | Berlin ; Heidelberg |
Publication type: | Article |
Language: | English |
Date of first publication: | 2022/01/28 |
Publication year: | 2022 |
Release date: | 2024/05/30 |
Tag: | 16S rRNA genes; Antarctica; James Ross archipelago; microbial diversity; microbial mats |
Volume: | 45 |
Issue: | 3 |
Number of pages: | 14 |
First page: | 405 |
Last Page: | 418 |
Funding institution: | Antarctic Science Ltd; Agencia Nacional de Promocion Cientifica y; Tecnologica (ANPCYT) [PICT 2017-2026]; Consejo Nacional de; Investigaciones Cientificas y Tecnicas (CONICET, Argentina) [PIP; 11220170100088CO]; Universidad Nacional de Cordoba (SeCyT, UNC); [336-20180100385-CB]; Consejo Nacional de Investigaciones Cientificas y; Tecnicas (CONICET, Argentina, PUE-CICTERRA 2016) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Geowissenschaften |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
License (German): | Keine öffentliche Lizenz: Unter Urheberrechtsschutz |