Anhui Wu, Annapurna Devi Allu, Prashanth Garapati, Hamad Siddiqui, Hakan Dortay, Maria-Ines Zanor, Maria Amparo Asensi-Fabado, Sergi Munne-Bosch, Carla Antonio, Takayuki Tohge, Alisdair Fernie, Kerstin Kaufmann, Gang-Ping Xue, Bernd Müller-Röber, Salma Balazadeh
- The transition from juvenility through maturation to senescence is a complex process that involves the regulation of longevity. Here, we identify JUNGBRUNNEN1 (JUB1), a hydrogen peroxide (H2O2)-induced NAC transcription factor, as a central longevity regulator in Arabidopsis thaliana. JUB1 overexpression strongly delays senescence, dampens intracellular H2O2 levels, and enhances tolerance to various abiotic stresses, whereas in jub1-1 knockdown plants, precocious senescence and lowered abiotic stress tolerance are observed. A JUB1 binding site containing a RRYGCCGT core sequence is present in the promoter of DREB2A, which plays an important role in abiotic stress responses. JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds directly to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species-responsive genes, including heat shock protein and glutathione S-transferase genes, whose expression is further induced byThe transition from juvenility through maturation to senescence is a complex process that involves the regulation of longevity. Here, we identify JUNGBRUNNEN1 (JUB1), a hydrogen peroxide (H2O2)-induced NAC transcription factor, as a central longevity regulator in Arabidopsis thaliana. JUB1 overexpression strongly delays senescence, dampens intracellular H2O2 levels, and enhances tolerance to various abiotic stresses, whereas in jub1-1 knockdown plants, precocious senescence and lowered abiotic stress tolerance are observed. A JUB1 binding site containing a RRYGCCGT core sequence is present in the promoter of DREB2A, which plays an important role in abiotic stress responses. JUB1 transactivates DREB2A expression in mesophyll cell protoplasts and transgenic plants and binds directly to the DREB2A promoter. Transcriptome profiling of JUB1 overexpressors revealed elevated expression of several reactive oxygen species-responsive genes, including heat shock protein and glutathione S-transferase genes, whose expression is further induced by H2O2 treatment. Metabolite profiling identified elevated Pro and trehalose levels in JUB1 overexpressors, in accordance with their enhanced abiotic stress tolerance. We suggest that JUB1 constitutes a central regulator of a finely tuned control system that modulates cellular H2O2 level and primes the plants for upcoming stress through a gene regulatory network that involves DREB2A.…
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MetadatenAuthor details: | Anhui Wu, Annapurna Devi AlluORCiD, Prashanth Garapati, Hamad Siddiqui, Hakan Dortay, Maria-Ines Zanor, Maria Amparo Asensi-Fabado, Sergi Munne-Bosch, Carla Antonio, Takayuki TohgeORCiD, Alisdair FernieORCiDGND, Kerstin KaufmannORCiD, Gang-Ping Xue, Bernd Müller-RöberORCiDGND, Salma BalazadehORCiDGND |
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DOI: | https://doi.org/10.1105/tpc.111.090894 |
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ISSN: | 1040-4651 |
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Title of parent work (English): | The plant cell |
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Publisher: | American Society of Plant Physiologists |
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Place of publishing: | Rockville |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2012 |
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Publication year: | 2012 |
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Release date: | 2017/03/26 |
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Volume: | 24 |
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Issue: | 2 |
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Number of pages: | 25 |
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First page: | 482 |
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Last Page: | 506 |
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Funding institution: | Deutsche Forschungsgemeinschaft [FOR 948, MU 1199/14-1];
Bundeministerium fur Bildung und Forschung [FKZ 0313924]; Chinese
Academy of Sciences [2009CB118604]; Pakistan Higher Education
Commission; Deutscher Akademischer Austauschdienst [DAAD A/02/37115];
International MaxPlanck Research School of the Max-Planck Society;
University of Potsdam; Institucio Catalana de Recerca i Estudis
Avancats; Generalitat de Catalunya; Ministry of Science and Innovation
of the Spanish Government [BFU2009-07294] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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Peer review: | Referiert |
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