- Trehalose 6-phosphate (Tre6P) is a sucrose signalling metabolite that has been implicated in regulation of shoot branching, but its precise role is not understood.
We expressed tagged forms of TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1) to determine where Tre6P is synthesized in arabidopsis (Arabidopsis thaliana), and investigated the impact of localized changes in Tre6P levels, in axillary buds or vascular tissues, on shoot branching in wild-type and branching mutant backgrounds.
TPS1 is expressed in axillary buds and the subtending vasculature, as well as in the leaf and stem vasculature. Expression of a heterologous Tre6P phosphatase (TPP) to lower Tre6P in axillary buds strongly delayed bud outgrowth in long days and inhibited branching in short days. TPP expression in the vasculature also delayed lateral bud outgrowth and decreased branching. Increased Tre6P in the vasculature enhanced branching and was accompanied by higher expression of FLOWERING LOCUS T (FT) and upregulation of sucrose transporters. Increased vascular Tre6PTrehalose 6-phosphate (Tre6P) is a sucrose signalling metabolite that has been implicated in regulation of shoot branching, but its precise role is not understood.
We expressed tagged forms of TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1) to determine where Tre6P is synthesized in arabidopsis (Arabidopsis thaliana), and investigated the impact of localized changes in Tre6P levels, in axillary buds or vascular tissues, on shoot branching in wild-type and branching mutant backgrounds.
TPS1 is expressed in axillary buds and the subtending vasculature, as well as in the leaf and stem vasculature. Expression of a heterologous Tre6P phosphatase (TPP) to lower Tre6P in axillary buds strongly delayed bud outgrowth in long days and inhibited branching in short days. TPP expression in the vasculature also delayed lateral bud outgrowth and decreased branching. Increased Tre6P in the vasculature enhanced branching and was accompanied by higher expression of FLOWERING LOCUS T (FT) and upregulation of sucrose transporters. Increased vascular Tre6P levels enhanced branching in branched1 but not in ft mutant backgrounds.
These results provide direct genetic evidence of a local role for Tre6P in regulation of axillary bud outgrowth within the buds themselves, and also connect Tre6P with systemic regulation of shoot branching via FT.…
MetadatenAuthor details: | Franziska FichtnerORCiDGND, Francois F. BarbierORCiD, Maria Grazia AnnunziataORCiD, Regina FeilORCiD, Justyna Jadwiga OlasORCiDGND, Bernd Müller-RöberORCiDGND, Mark StittORCiDGND, Christine A. BeveridgeORCiD, John Edward LunnORCiD |
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URN: | urn:nbn:de:kobv:517-opus4-569564 |
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DOI: | https://doi.org/10.25932/publishup-56956 |
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ISSN: | 1866-8372 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/33068448 |
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Title of parent work (German): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe |
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Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (1383) |
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Publication type: | Postprint |
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Language: | English |
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Date of first publication: | 2020/11/25 |
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Publication year: | 2020 |
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Publishing institution: | Universität Potsdam |
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Release date: | 2024/03/19 |
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Tag: | Arabidopsis thaliana (arabidopsis); axillary bud; branching; sucrose; sugar signalling; trehalose 6‐ phosphate (Tre6P) |
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Issue: | 1383 |
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Number of pages: | 19 |
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Source: | New Phytol, 229: 2135-2151. https://doi.org/10.1111/nph.17006 |
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Funding institution: | International Max Planck Research School - Primary Metabolism and Plant; Growth; Australian Research CouncilAustralian Research Council; [DP150102086, FL180100139]; Max Planck SocietyMax Planck; SocietyFoundation CELLEX; Deutsche Forschungsgemeinschaft (DFG; within; the Collaborative Research Centre 973) |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Green Open-Access |
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License (German): | CC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International |
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External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |
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