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Application of Laser Postionization Secondary Neutral Mass Spectrometry/Time-of-Flight Secondary Ion Mass Spectrometry in Nanotoxicology: Visualization of Nanosilver in Human Macrophages and Cellular Responses

  • Silver nanoparticles (SNP) are the subject of worldwide commercialization because of their antimicrobial effects. Yet only little data on their mode of action exist. Further, only few techniques allow for visualization and quantification of unlabeled nanoparticles inside cells. To study SNP of different sizes and coatings within human macrophages, we introduce a novel laser postionization secondary neutral mass spectrometry (Laser-SNMS) approach and prove this method superior to the widely applied confocal Raman and transmission electron microscopy. With time-of-flight secondary ion mass spectrometry (TOF-SIMS) we further demonstrate characteristic fingerprints in the lipid pattern of the cellular membrane indicative of oxidative stress and membrane fluidity changes. Increases of protein carbonyl and heme oxygenase-1 levels in treated cells confirm the presence of oxidative stress biochemically. Intriguingly, affected phagocytosis reveals as highly sensitive end point of SNP-mediated adversity In macrophages. The cellular responsesSilver nanoparticles (SNP) are the subject of worldwide commercialization because of their antimicrobial effects. Yet only little data on their mode of action exist. Further, only few techniques allow for visualization and quantification of unlabeled nanoparticles inside cells. To study SNP of different sizes and coatings within human macrophages, we introduce a novel laser postionization secondary neutral mass spectrometry (Laser-SNMS) approach and prove this method superior to the widely applied confocal Raman and transmission electron microscopy. With time-of-flight secondary ion mass spectrometry (TOF-SIMS) we further demonstrate characteristic fingerprints in the lipid pattern of the cellular membrane indicative of oxidative stress and membrane fluidity changes. Increases of protein carbonyl and heme oxygenase-1 levels in treated cells confirm the presence of oxidative stress biochemically. Intriguingly, affected phagocytosis reveals as highly sensitive end point of SNP-mediated adversity In macrophages. The cellular responses monitored are. hierarchically linked, but follow individual kinetics and are partially reversible.show moreshow less

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Metadaten
Author:Andrea Haase, Heinrich F. Arlinghaus, Jutta Tentschert, Harald Jungnickel, Philipp Graf, Alexandre Mantion, Felix Draude, Sebastian Galla, Johanna Plendl, Mario E. Goetz, Admir Masic, Wolfgang P. Meier, Andreas F. Thuenemann, Andreas TaubertORCiDGND, Andreas Luch
DOI:https://doi.org/10.1021/nn200163w
ISSN:1936-0851 (print)
Parent Title (English):ACS nano
Publisher:American Chemical Society
Place of publication:Washington
Document Type:Article
Language:English
Year of first Publication:2011
Year of Completion:2011
Release Date:2017/03/26
Tag:Laser-SNMS; TOF-SIMS; confocal Raman microscopy; nanosilver; oxidative stress; protein carbonyls
Volume:5
Issue:4
Pagenumber:10
First Page:3059
Last Page:3068
Funder:Alexander von Humboldt Foundation; Max-Planck-Society; Federal Ministry of Education and Research; Federal Institute of Risk Assessment; Federal Institute for Materials Research and Testing; Swiss National Science Foundation; NCCR Nanoscience; University of Potsdam; Free University of Berlin; Fond der Chemischen Industrie; MPI of Colloids and Interfaces (Colloid Chemistry Department)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Peer Review:Referiert