MIP-esterase/Tyrosinase Combinations for Paracetamol and Phenacetin
- A new electrochemical MIP sensor for the most frequently used drug paracetamol (PAR) was prepared by electropolymerization of mixtures containing the template molecule and the functional monomers ophenylenediamine, resorcinol and aniline. The imprinting factor of 12 reflects the effective target binding to the MIP as compared with the non-imprinted electropolymer. Combination of the MIP with a nonspecific esterase allows the measurement of phenacetin - another analgesic drug. In the second approach the PAR containing sample solution was pretreated with tyrosinase in order to prevent electrochemical interferences by ascorbic acid and uric acid. Interference-free indication at a very low electrode potential without fouling of the electrode surface was achieved with the o-phenylenediamine: resorcinol-based MIP.
| Author details: | Aysu YarmanORCiDGND, Frieder W. SchellerORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1002/elan.201600042 |
| ISSN: | 1040-0397 |
| ISSN: | 1521-4109 |
| Title of parent work (English): | Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2016 |
| Publication year: | 2016 |
| Release date: | 2020/03/22 |
| Tag: | Electropolymerization; Esterase; Molecularly imprinted polymers; Paracetamol; Phenacetin; Tyrosinase |
| Volume: | 28 |
| Number of pages: | 6 |
| First page: | 2222 |
| Last Page: | 2227 |
| Funding institution: | Deutsche Forschungsgemeinschaft (DFG) within the framework of the German Excellence Initiative [EXC 314]; BMBF in TERA-Sens [93719903] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
