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Bioelectrocatalysis by Microperoxidase-11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles
- We report on the redox behaviour of the microperoxidase-11 (MP-11) which has been electrostatically immobilized in a matrix of chitosan-embedded gold nanoparticles on the surface of a glassy carbon electrode. MP-11 contains a covalently bound heme c as the redox active group that exchanges electrons with the electrode via the gold nanoparticles. Electroactive surface concentration of MP-11 at high scan rate is between 350+/-50 pmol cm(-2), which reflects a multilayer process. The formal potential (E degrees') of MP-11 in the gold nanoparticles-chitosan film was estimated to be -(267.7+/-2.9) mV at pH 7.0. The heterogeneous electron transfer rate constant (k(s)) starts at 1.21 s(-1) and levels off at 6.45 s(-1) in the scan rate range from 0.1 to 2.0 V s(-1). Oxidation and reduction of MP-11 by hydrogen peroxide and superoxide, respectively have been coupled to the direct electron transfer of MP-11.
Verfasserangaben: | Aysu YarmanORCiDGND, Thomas Nagel, Nenad Gajovic-Eichelmann, Anna Fischer, Ursula WollenbergerORCiDGND, Frieder W. SchellerORCiDGND |
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DOI: | https://doi.org/10.1002/elan.201000535 |
ISSN: | 1040-0397 |
Titel des übergeordneten Werks (Englisch): | Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis |
Verlag: | Wiley-Blackwell |
Verlagsort: | Malden |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2011 |
Erscheinungsjahr: | 2011 |
Datum der Freischaltung: | 26.03.2017 |
Freies Schlagwort / Tag: | Bioelectrocatalysis; Direct electron transfer; Hydrogen peroxide; Microperoxidase; Nanoparticles; Superoxide |
Band: | 23 |
Ausgabe: | 3 |
Seitenanzahl: | 8 |
Erste Seite: | 611 |
Letzte Seite: | 618 |
Fördernde Institution: | BMBF of Germany [0311993]; Deutsche Forschunsgeminschaft (DFG) within the framework of the German Excellence Initiative [EXC 314] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer Review: | Referiert |