Verification and biophysical characterization of a New Three-Color Forster Resonance-Energy-Transfer (FRET) System in DNA
- We report on a new three-color FRET system consisting of three fluorescent dyes, i.e., of a carbostyril (=quinolin-2(1H)-one)-derived donor D, a (bathophenanthroline)ruthenium complex as a relay chromophore A1, and a Cy dye as A2 (FRET=Forster resonance-energy-transfer) (cf. Fig. 1). With their widely matching spectroscopic properties (cf. Fig. 2), the combination of these dyes yielded excellent FRET efficiencies. Furthermore, fluorescence lifetime measurements revealed that the long fluorescence lifetime of the Ru complex was transferred to the Cy dye offering the possibility to measure the whole system in a time-resolved mode. The FRET system was established on double-stranded DNA (cf. Fig. 3) but it should also be generally applicable to other biomolecules.
| Author details: | Andrea Altevogt Nee Kienzler, Roman FlehrGND, Sören Gehne, Michael Uwe KumkeORCiDGND, Willi Bannwarth |
|---|---|
| DOI: | https://doi.org/10.1002/hlca.201100460 |
| ISSN: | 0018-019X |
| Title of parent work (English): | Helvetica chimica acta |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2012 |
| Publication year: | 2012 |
| Release date: | 2017/03/26 |
| Tag: | DNA; Fluorescence; Forster resonance energy transfer (FRET) system; Ruthenium complexes |
| Volume: | 95 |
| Issue: | 4 |
| Number of pages: | 13 |
| First page: | 543 |
| Last Page: | 555 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Peer review: | Referiert |
