System-wide organization of actin cytoskeleton determines organelle transport in hypocotyl plant cells
- The actin cytoskeleton is an essential intracellular filamentous structure that underpins cellular transport and cytoplasmic streaming in plant cells. However, the system-level properties of actin-based cellular trafficking remain tenuous, largely due to the inability to quantify key features of the actin cytoskeleton. Here, we developed an automated image-based, network-driven framework to accurately segment and quantify actin cytoskeletal structures and Golgi transport. We show that the actin cytoskeleton in both growing and elongated hypocotyl cells has structural properties facilitating efficient transport. Our findings suggest that the erratic movement of Golgi is a stable cellular phenomenon that might optimize distribution efficiency of cell material. Moreover, we demonstrate that Golgi transport in hypocotyl cells can be accurately predicted from the actin network topology alone. Thus, our framework provides quantitative evidence for system-wide coordination of cellular transport in plant cells and can be readily applied toThe actin cytoskeleton is an essential intracellular filamentous structure that underpins cellular transport and cytoplasmic streaming in plant cells. However, the system-level properties of actin-based cellular trafficking remain tenuous, largely due to the inability to quantify key features of the actin cytoskeleton. Here, we developed an automated image-based, network-driven framework to accurately segment and quantify actin cytoskeletal structures and Golgi transport. We show that the actin cytoskeleton in both growing and elongated hypocotyl cells has structural properties facilitating efficient transport. Our findings suggest that the erratic movement of Golgi is a stable cellular phenomenon that might optimize distribution efficiency of cell material. Moreover, we demonstrate that Golgi transport in hypocotyl cells can be accurately predicted from the actin network topology alone. Thus, our framework provides quantitative evidence for system-wide coordination of cellular transport in plant cells and can be readily applied to investigate cytoskeletal organization and transport in other organisms.…
Author details: | David BreuerGND, Jacqueline Nowak, Alexander Ivakov, Marc Somssich, Staffan PerssonORCiDGND, Zoran NikoloskiORCiDGND |
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DOI: | https://doi.org/10.1073/pnas.1706711114 |
ISSN: | 0027-8424 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/28655850 |
Title of parent work (English): | Proceedings of the National Academy of Sciences of the United States of America |
Publisher: | National Acad. of Sciences |
Place of publishing: | Washington |
Publication type: | Article |
Language: | English |
Year of first publication: | 2017 |
Publication year: | 2017 |
Release date: | 2020/04/20 |
Tag: | Golgi; actin; cytoskeleton; image processing; networks |
Volume: | 114 |
Number of pages: | 9 |
First page: | E5741 |
Last Page: | E5749 |
Funding institution: | International Max Planck Research School scholarship; Max Planck Society; Australian Research Council [FT160100218]; R@MAP Professorship at University of Melbourne; Dyason travel grant |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |