David Botero, Jonathan Monk, Maria Juliana Rodriguez Cubillos, Andres Eduardo Rodriguez Cubillos, Mariana Restrepo, Vivian Bernal-Galeano, Alejandro Reyes, Andres Gonzalez Barrios, Bernhard O. Palsson, Silvia Restrepo, Adriana Bernal
- Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, the most important bacterial disease in this crop. There is a paucity of knowledge about the metabolism of Xanthomonas and its relevance in the pathogenic process, with the exception of the elucidation of the xanthan biosynthesis route. Here we report the reconstruction of the genome-scale model of Xpm metabolism and the insights it provides into plant-pathogen interactions. The model, iXpm1556, displayed 1,556 reactions, 1,527 compounds, and 890 genes. Metabolic maps of central amino acid and carbohydrate metabolism, as well as xanthan biosynthesis of Xpm, were reconstructed using Escher (https://escher.github.io/) to guide the curation process and for further analyses. The model was constrained using the RNA-seq data of a mutant of Xpm for quorum sensing (QS), and these data were used to construct context-specific models (CSMs) of the metabolism of the two strains (wild type and QS mutant). The CSMs and flux balance analysis were used to getXanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, the most important bacterial disease in this crop. There is a paucity of knowledge about the metabolism of Xanthomonas and its relevance in the pathogenic process, with the exception of the elucidation of the xanthan biosynthesis route. Here we report the reconstruction of the genome-scale model of Xpm metabolism and the insights it provides into plant-pathogen interactions. The model, iXpm1556, displayed 1,556 reactions, 1,527 compounds, and 890 genes. Metabolic maps of central amino acid and carbohydrate metabolism, as well as xanthan biosynthesis of Xpm, were reconstructed using Escher (https://escher.github.io/) to guide the curation process and for further analyses. The model was constrained using the RNA-seq data of a mutant of Xpm for quorum sensing (QS), and these data were used to construct context-specific models (CSMs) of the metabolism of the two strains (wild type and QS mutant). The CSMs and flux balance analysis were used to get insights into pathogenicity, xanthan biosynthesis, and QS mechanisms. Between the CSMs, 653 reactions were shared; unique reactions belong to purine, pyrimidine, and amino acid metabolism. Alternative objective functions were used to demonstrate a trade-off between xanthan biosynthesis and growth and the re-allocation of resources in the process of biosynthesis. Important features altered by QS included carbohydrate metabolism, NAD(P)(+) balance, and fatty acid elongation. In this work, we modeled the xanthan biosynthesis and the QS process and their impact on the metabolism of the bacterium. This model will be useful for researchers studying host-pathogen interactions and will provide insights into the mechanisms of infection used by this and other Xanthomonas species.…
MetadatenAuthor details: | David BoteroORCiD, Jonathan Monk, Maria Juliana Rodriguez Cubillos, Andres Eduardo Rodriguez CubillosORCiDGND, Mariana RestrepoORCiD, Vivian Bernal-GaleanoORCiD, Alejandro ReyesORCiD, Andres Gonzalez Barrios, Bernhard O. PalssonGND, Silvia Restrepo, Adriana Bernal |
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DOI: | https://doi.org/10.3389/fgene.2020.00837 |
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ISSN: | 1664-8021 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32849823 |
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Title of parent work (English): | Frontiers in genetics |
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Subtitle (English): | an approach to elucidate pathogenicity at the metabolic level |
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Publisher: | Frontiers Media |
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Place of publishing: | Lausanne |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2020/08/11 |
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Publication year: | 2020 |
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Release date: | 2024/01/19 |
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Tag: | Xanthomonas; Xpm; cassava bacterial blight; genome-scale metabolic; model; quorum sensing |
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Volume: | 11 |
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Article number: | 837 |
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Number of pages: | 19 |
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Funding institution: | Administrative Department of Science and Technology in Colombia; (Colciencias)Departamento Administrativo de Ciencia, Tecnologia e; Innovacion Colciencias [567]; ColcienciasDepartamento Administrativo de; Ciencia, Tecnologia e Innovacion Colciencias [0619, 2013]; Faculty of; Sciences [INV-2019-84-1855] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
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Peer review: | Referiert |
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Publishing method: | Open Access / Gold Open-Access |
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| DOAJ gelistet |
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License (German): | CC-BY - Namensnennung 4.0 International |
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